Open Access (OA) has emerged as a pivotal driver shaping the dissemination scope and academic impact of research findings. To clarify the impact of publishing models such as open access on the citation performance of biochemical papers, this study selects 177,745 biochemistry professional papers included in the core collection of the Web of Science (WoS CC) as the research data; we conduct an analysis of citation and impact advantages in biochemistry research. Employing correlation analysis, baseline regression modeling, and two-way ANOVA, our analysis indicates that: OA publications in biochemistry exhibit notable citation and impact advantages, which are positively correlated with the degree of openness, and the key determinants of the OA advantage encompass funding sources, reference count, and publication region. At present, China accounts for a disproportionately small proportion of OA papers in this field. In the context of the open-science paradigm, Chinese academic journals must systematically address their developmental bottlenecks and formulate publication innovation strategies to enhance the quality of academic publishing.