The cornea is the outermost layer of the eye and is constantly exposed to trauma due to its anatomical location. Propolis is a substance produced by honeybees by mixing the extracts they collect from plants with their secretions. Studies have shown that propolis contains essential biological active substances for the life of organisms, which enhance epithelialization and have strong analgesic, anti-inflammatory, immunomodulatory, antioxidant, antitumor, antibacterial, antifungal, and antiviral effects. In our study, we created a corneal wound with a diameter of 3 mm using a corneal blade. In the experimentally induced corneal wound, no treated the first group, the second group was treated with nanofibers containing propolis produced by the electrospinning method, and the third group treated water-based topical propolis application. Topical propolis was applied once a day for 3 days, while nanofibers containing propolis were applied once following wound formation. Fluorescein staining was performed on the rats eyes every day throughout the study, and photographs were taken to measure the wound sizes. On the third day, the rats were euthanized under general anesthesia, and histopathological examination was performed on their corneas. In terms of bleeding, no significant difference was observed between the propolis and control groups, while a lower level of bleeding was detected in the nanopropolis group. Propolis and nanofibers containing propolis groups showed a significantly positive effect on wound healing compared to the control group.

BACKGROUND: Wound healing involves the repair of skin and other soft tissues after an injury. Royal jelly, a product of bees, possesses antioxidant, anti-inflammatory, antibacterial, and antiviral properties. Melatonin, a circadian indoleamine, is produced in the pineal gland and other organs. This study explores the effects of melatonin and royal jelly, both individually and combined, on wound healing in geriatric and young mice. METHODS: The study includes 90 Balb/C mice divided into ten groups to assess the effects of royal jelly and melatonin on wound healing. Royal jelly was applied topically at a concentration of 300 mg/kg. Melatonin was formulated in a vaseline-based pomade at a concentration of 5 mg/kg. The substances were applied either separately or in combination to wounds created on the mice. RESULTS: Both substances significantly enhanced wound healing at a macroscopic level in both age groups. Melatonin was found to be more effective during the initial wound formation process, whereas royal jelly was more beneficial during the granulation phase. However, significant results at a histopathological level were observed only in geriatric animals. CONCLUSION: The findings suggest a potential new therapeutic approach to enhance wound healing, particularly in elderly individuals. However, these findings need to be supported through further research and clinical trials.

This study aimed to determine the anesthetic efficacy of intranasal medetomidine/ketamine and diazepam/ketamine combinations in New Zealand White rabbits. For this purpose, 12 adult New Zealand White rabbits weighing 2.3‐4.1 kg were used by randomly dividing the animals into two groups of six animals: Medetomidine ‐ Ketamine (MK) and Diazepam ‐ Ketamine (DK). Intranasally, medetomidine (0.2 mg kg‐1) and ketamine (10 mg kg‐1) combination was administered to the MK group and, diazepam (1 mg kg‐1) and ketamine (10 mg kg‐1) to the DK group. Before and 5, 10, 15, 20, 25, 30, 45, and 60 minutes after anesthesia, loss, and recovery of the reflexes were evaluated. Onset and duration of the anesthesia, duration of the surgical anesthesia, and the total anesthesia time were also recorded. Loss and recovery time of reflexes was shorter in the DK group compared to the MK group. Respiratory rate, heart rate, and SpO2 decreased as expected in both groups but all parameters, especially SpO2, were lower in the MK group comparing the DK group. These results showed that both MK and DK combinations administered IN provide anesthesia in the time and depth that minor surgical procedures and clinical applications could be performed. Although it seems promising considering its practicality and anesthetic efficacy, it is clear that more detailed studies with more animals are needed before the safety of these protocols can be established.Their use, recommended for routine clinical procedures when considered SpO2, was severely decreased, especially in the MK group.

The aim of this study was to investigate the regenerative effect of lyophilized dental follicle mesenchymal stem cells (DF-MSCs) combined with rat platelet-rich fibrin (PRF) on geriatric skin wounds. Human DF-MSCs which were isolated from the wisdom teeth of healthy donors and PRF were mixed and incubated in a 37 °C incubator for 1–2 h containing 1 million cells in 150 mg PRF. The mixture was suspended in a freeze-drying solution and then lyophilized. Wounds were created on the back skin of Wistar albino rats using a 6 mm punch. Lyophilized DF-MSCs, PRF, or PRF + DF-MSCs were applied to the wounds of rats. On the 15th day, the wound area was histopathologically evaluated in rats. Blood samples from rats were analyzed for total antioxidant status (TAOS), and inflammatory cytokine levels using ELISA. In both young and geriatric rats treated with lyophilized PRF + DF-MSCs, wound area began to significantly decrease from the 10th day compared to the untreated group (p < 0.05). Histopathological examination revealed that in the lyophilized PRF + DF-MSCs treated groups, epithelial integrity and scarless healing significantly increased compared to the untreated groups (p < 0.05). There were no significant differences in TAOS, total oxidant status (TOS), tumor necrosis factor (TNF), interleukin-6 (IL6), and hydroxyproline levels in serum samples from young rats on the 15th day. In geriatric rats, hydroxyproline (HYPS) levels were increased in the DF-MSC and PRF + DF-MSC groups (p < 0.01), TNF was significantly elevated in PRF geriatric group and IL6 was increased in the PRF group compared to the control group (p = 0.01). Lyophilized PRF + DF-MSCs, which is a shelf-stable and ready-to-use product, hold promise, especially for traumatic wounds in geriatric individuals with longer healing times.

Mesenchymal stem cells (MSCs) are candidates for the treatment of palatal wounds in combination with biomaterials. In this study, we developed a method for the production of a ready-to-use mucoadhesive hydrogel containing MSCs for palatal wounds and evaluated its healing effects. Dental follicle MSCs (DFMSCs) were isolated from the dental follicle tissue of a healthy twenty-year-old donor. DFMSCs were suspended in a cell-preserving solution containing platelet-rich plasma, trehalose, and DMSO, and loaded into a catechol–chitosan hydrogel solution at a ratio of 1:400 (v/v) with 5 × 10⁵ or 6 × 10⁶ cells per hydrogel to create a novel lyophilization method for cell integration into the biomaterial. Hydrogels were fabricated as scaffolds with a diameter of 5 mm and a depth of 4 mm. After lyophilization of the hydrogels with cells, a viability test was performed after the production of hydrogels on the seventh day and the fifth month. Palatal wounds were created as full-thickness wounds in rats using a 5 mm diameter punch. The hydrogels were applied to the palatal wounds of rats, and histochemical and immunohistochemical analyses were performed. The results showed that, after rehydration of the hydrogels, DFMSCs had over 80% viability and were homogeneously distributed in the hydrogels. After the application of DFMSC-loaded hydrogels, palatal wounds healed within 7 days, and inflammatory cell infiltration, fibroblastic proliferation, and ulceration were significantly reduced, while epithelial regeneration was significantly increased compared to the control group. The viability ratio of DFMSCs was 83.7% on the seventh day and 71.3% in the fifth month. Hydrogels loaded with DFMSCs represent a promising, ready-to-use biomaterial approach for supporting palatal wound healing.

Kök hücreler kendilerini yenileme özelliğine sahip olup, özel koşullar altında farklı hücre tiplerine dönüşebilmektedir. Kök hücreler farklılaşma özelliklerine göre; totipotent kök hücreler, pluripotent kök hücreler ve multipotent kök hücreler olarak sınıflandırılırken, embriyonik ve embriyonik olmayan kök hücreler olmak üzere iki ana gruba ayrılmaktadır. Bu derlemede kök hücrelerin genel özellikleri ile kök hücre tiplerinden bahsedilerek veteriner hekimlikte kullanım alanları hakkında özlü bilgi verilmesi amaçlanmıştır.