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Dr. YunJie Ruan
Institute of Agricultural Bio-Environmental Engineering, College of Bio-Systems Engineering and Food Science/The Rural Development Academy, Zhejiang University, Hangzhou 310058, China

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0 Resource Recovery
0 Waste Water Treatment
0 Nitrification
0 Denitrification
0 Biological treatment

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Denitrification
Nitrification

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Journal article
Published: 26 July 2021 in Microorganisms
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Aerobic denitrification, one of the important nitrate metabolic pathways in biological denitrification, has been attracting increasing interest recently due to its functional advantages. In order to evaluate the effect of antibiotics on aerobic denitrification and guide practical engineering application of aerobic denitrification techniques, we evaluated the performance of aerobic denitrification by the strain Pseudomonas balearica RAD-17 in the presence of ciprofloxacin (CFX) and oxytetracycline (OTC). No significant negative impact on the performance of aerobic denitrification in the presence of CFX or OTC within the range of 50 to 300 μg L−1 was found. Significant degradation of OTC was found within the range of 50 μg L−1 to 300 μg L−1 under aerobic denitrification conditions, while no degradation was found for CFX. Stimulation of cell growth occurred within the investigated range of antibiotics. Under anoxic or aerobic conditions, the addition of CFX or OTC changed the N2O production trend. The results in the present study may play an important role in informing the use of aerobic denitrification techniques in the presence of antibiotics within environmentally relevant concentrations (<1 mg/L).

ACS Style

Yunjie Ruan; Lei Cai; Huifeng Lu; Meng Zhang; Xiangyang Xu; Wenbing Li. Performance of Aerobic Denitrification by the Strain Pseudomonas balearica RAD-17 in the Presence of Antibiotics. Microorganisms 2021, 9, 1584 .

AMA Style

Yunjie Ruan, Lei Cai, Huifeng Lu, Meng Zhang, Xiangyang Xu, Wenbing Li. Performance of Aerobic Denitrification by the Strain Pseudomonas balearica RAD-17 in the Presence of Antibiotics. Microorganisms. 2021; 9 (8):1584.

Chicago/Turabian Style

Yunjie Ruan; Lei Cai; Huifeng Lu; Meng Zhang; Xiangyang Xu; Wenbing Li. 2021. "Performance of Aerobic Denitrification by the Strain Pseudomonas balearica RAD-17 in the Presence of Antibiotics." Microorganisms 9, no. 8: 1584.

Journal article
Published: 28 December 2020 in Bioresource Technology
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This study investigated the response of nitrogen removal performance and microbial community to different carbon composites in biofilm airlift reactors for wastewater treatment. Three reactors were filled with poly (butylene succinate) and bamboo powder composite at the blending ratio of 9:1, 1:1 and 1:9. Increasing the component of bamboo powder in the carrier reduced the carbon availability and had an adverse effect on nitrate removal efficiency. However, bamboo powder improved the ammonia removal rate which mainly through autotrophic nitrification. Three reactors exhibited distinct microbial compositions in both bacterial and fungal diversity. High inclusion of bamboo power decreased the relative abundance of denitrifiers Denitromonas and increased the relative abundance of nitrifiers, including Nitromonas, Nitrospina and Nitrospira. Moreover, correlation network revealed a competitive interaction between the taxa responsible for ammonia removal and nitrate removal processes. Those results indicated the feasibility of steering nitrogen removal pathway through carrier formulation in wastewater treatment.

ACS Style

Yale Deng; Yunjie Ruan; Mohammad J. Taherzadeh; Jishuang Chen; Wanhe Qi; Dedong Kong; Bin Ma; Xiangyang Xu; Huifeng Lu. Carbon availability shifts the nitrogen removal pathway and microbial community in biofilm airlift reactor. Bioresource Technology 2020, 323, 124568 .

AMA Style

Yale Deng, Yunjie Ruan, Mohammad J. Taherzadeh, Jishuang Chen, Wanhe Qi, Dedong Kong, Bin Ma, Xiangyang Xu, Huifeng Lu. Carbon availability shifts the nitrogen removal pathway and microbial community in biofilm airlift reactor. Bioresource Technology. 2020; 323 ():124568.

Chicago/Turabian Style

Yale Deng; Yunjie Ruan; Mohammad J. Taherzadeh; Jishuang Chen; Wanhe Qi; Dedong Kong; Bin Ma; Xiangyang Xu; Huifeng Lu. 2020. "Carbon availability shifts the nitrogen removal pathway and microbial community in biofilm airlift reactor." Bioresource Technology 323, no. : 124568.

Journal article
Published: 19 February 2020 in Bioresource Technology
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This study explored the denitrification performance of solid-phase denitrification (SPD) systems packed with poly (butylene succinate)/bamboo powder composite to treat synthetic aquaculture wastewater under different salinity conditions (0‰ Vs. 25‰). The results showed composite could achieve the maximum denitrification rates of 0.22 kg (salinity, 0‰) and 0.34 kg NO3−-N m−3 d−1 (salinity, 25‰) over 200-day operation. No significant nitrite accumulation and less dissolved organic carbon (DOC) release (<15 mg/L) were found. The morphological and spectroscopic analyses demonstrated the mixture composites degradation. Microbial community analysis showed that Acidovorax, Simplicispira, Denitromonas, SM1A02, Marinicella and Formosa were the dominant genera for denitrifying bacteria, while Aspergillus was the major genus for denitrifying fungus. The co-network analysis also indicated the interactions between bacterial and fungal community played an important role in composite degradation and denitrification. The outcomes provided a potential strategy of DOC control and cost reduction for aquaculture nitrate removal by SPD.

ACS Style

Wanhe Qi; Mohammad J. Taherzadeh; Yunjie Ruan; Yale Deng; Ji-Shuang Chen; Hui-Feng Lu; Xiang-Yang Xu. Denitrification performance and microbial communities of solid-phase denitrifying reactors using poly (butylene succinate)/bamboo powder composite. Bioresource Technology 2020, 305, 123033 .

AMA Style

Wanhe Qi, Mohammad J. Taherzadeh, Yunjie Ruan, Yale Deng, Ji-Shuang Chen, Hui-Feng Lu, Xiang-Yang Xu. Denitrification performance and microbial communities of solid-phase denitrifying reactors using poly (butylene succinate)/bamboo powder composite. Bioresource Technology. 2020; 305 ():123033.

Chicago/Turabian Style

Wanhe Qi; Mohammad J. Taherzadeh; Yunjie Ruan; Yale Deng; Ji-Shuang Chen; Hui-Feng Lu; Xiang-Yang Xu. 2020. "Denitrification performance and microbial communities of solid-phase denitrifying reactors using poly (butylene succinate)/bamboo powder composite." Bioresource Technology 305, no. : 123033.

Journal article
Published: 02 January 2020 in Microorganisms
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An aerobic denitrification strain, Pseudomonas balearica RAD-17, was identified and showed efficient inorganic nitrogen removal ability. The average NO3−-N, NO2−-N, and total ammonium nitrogen (TAN) removal rate (>95% removal efficiency) in a batch test was 6.22 mg/(L∙h), 6.30 mg/(L∙h), and 1.56 mg/(L∙h), respectively. Meanwhile, optimal incubate conditions were obtained through single factor experiments. For nitrogen removal pathways, the transcriptional results proved that respiratory nitrate reductases encoded by napA, which was primarily performed in aerobic denitrification and cell assimilation, were conducted by gluS and gluD genes for ammonium metabolism. In addition, adding the strain RAD-17 into actual wastewater showed obvious higher denitrification performance than in the no inoculum group (84.22% vs. 22.54%), and the maximum cell abundance achieved 28.5 ± 4.5% in a ratio of total cell numbers. Overall, the efficient nitrogen removal performance plus strong environmental fitness makes the strain RAD-17 a potential alternative for RAS (recirculating aquaculture system) effluent treatment.

ACS Style

Yunjie Ruan; Mohammad J. Taherzadeh; Dedong Kong; Huifeng Lu; Heping Zhao; Xiangyang Xu; Yu Liu; Lei Cai. Nitrogen Removal Performance and Metabolic Pathways Analysis of a Novel Aerobic Denitrifying Halotolerant Pseudomonas balearica Strain RAD-17. Microorganisms 2020, 8, 72 .

AMA Style

Yunjie Ruan, Mohammad J. Taherzadeh, Dedong Kong, Huifeng Lu, Heping Zhao, Xiangyang Xu, Yu Liu, Lei Cai. Nitrogen Removal Performance and Metabolic Pathways Analysis of a Novel Aerobic Denitrifying Halotolerant Pseudomonas balearica Strain RAD-17. Microorganisms. 2020; 8 (1):72.

Chicago/Turabian Style

Yunjie Ruan; Mohammad J. Taherzadeh; Dedong Kong; Huifeng Lu; Heping Zhao; Xiangyang Xu; Yu Liu; Lei Cai. 2020. "Nitrogen Removal Performance and Metabolic Pathways Analysis of a Novel Aerobic Denitrifying Halotolerant Pseudomonas balearica Strain RAD-17." Microorganisms 8, no. 1: 72.

Journal article
Published: 26 December 2019 in Water
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Integrated multi trophic aquaculture (IMTA) improves the production of aquatic animals by promoting nutrient utilization through different tropical levels. Microorganisms play an important role in elements cycling, energy flow and farmed-species health. The aim of this study was to evaluate how feed types, fresh frozen fish diet (FFD) or formulated diet (FD), influence the microbial community diversity and functionality in both water and sediment in a marine IMTA system. Preferable water quality, higher animal yields and higher cost efficiency were achieved in the FD pond. Feed types changed the pond bacterial community distribution, especially in the rearing water. The FFD pond was dominated with Cyanobacteria in the water, which played an important role in nitrogen fixation through photosynthesis due to the high nitrogen input of the frozen fish diet. The high carbohydrate composition in the formulated diet triggered higher metabolic pathways related to carbon and lipid metabolism in the water of the FD pond. Sediment had significantly higher microbial diversity than the rearing water. In sediment, the dominating genus, Sulfurovum and Desulfobulbus, were found to be positively correlated by network analysis, which had similar functionality in sulfur transformation. The relatively higher rates of antibiotic biosynthesis in the FFD sediment might be related to the pathogenic bacteria introduced by the trash fish diet. The difference in microbial community composition and metabolic pathways may be associated with the different pathways for nutrient cycling and animal growth performance. The formulated diet was determined to be more ecologically and economically sustainable than the frozen fish diet for marine IMTA pond systems.

ACS Style

Yale Deng; Fan Zhou; Yunjie Ruan; Bin Ma; Xueyan Ding; Xiaomei Yue; Wenjun Ma; Xuwang Yin; Ma. Feed Types Driven Differentiation of Microbial Community and Functionality in Marine Integrated Multitrophic Aquaculture System. Water 2019, 12, 95 .

AMA Style

Yale Deng, Fan Zhou, Yunjie Ruan, Bin Ma, Xueyan Ding, Xiaomei Yue, Wenjun Ma, Xuwang Yin, Ma. Feed Types Driven Differentiation of Microbial Community and Functionality in Marine Integrated Multitrophic Aquaculture System. Water. 2019; 12 (1):95.

Chicago/Turabian Style

Yale Deng; Fan Zhou; Yunjie Ruan; Bin Ma; Xueyan Ding; Xiaomei Yue; Wenjun Ma; Xuwang Yin; Ma. 2019. "Feed Types Driven Differentiation of Microbial Community and Functionality in Marine Integrated Multitrophic Aquaculture System." Water 12, no. 1: 95.

Journals
Published: 17 December 2019 in Lab on a Chip
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Electro-coalescence in step emulsification provides good control over the morphology and sizes of the Janus/Multi-layered droplets.

ACS Style

Xing Huang; Binbin He; Zhongbin Xu; Hao Pei; Yun Jie Ruan. Electro-coalescence in step emulsification: dynamics and applications. Lab on a Chip 2019, 20, 592 -600.

AMA Style

Xing Huang, Binbin He, Zhongbin Xu, Hao Pei, Yun Jie Ruan. Electro-coalescence in step emulsification: dynamics and applications. Lab on a Chip. 2019; 20 (3):592-600.

Chicago/Turabian Style

Xing Huang; Binbin He; Zhongbin Xu; Hao Pei; Yun Jie Ruan. 2019. "Electro-coalescence in step emulsification: dynamics and applications." Lab on a Chip 20, no. 3: 592-600.

Journal article
Published: 01 November 2019 in Environment International
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Suspended floc and fixed biofilm are two commonly applied strategies for heterotrophic denitrification in wastewater treatment. These two strategies use different carbon sources and reside within different ecological niches for microbial aggregation, which were hypothesized to show distinct microbial structures and metabolic fitness. We surveyed three floc reactors and three biofilm reactors for denitrification and determined if there were distinct microbial aggregations. Multiple molecular omics approaches were used to determine the microbial community composition, co-occurrence network and metabolic pathways. Proteobacteria was the dominating and most active phylum among all samples. Carbon source played an important role in shaping the microbial community composition while the distribution of functional protein was largely influenced by salinity. We found that the topological network features had different ecological patterns and that the microorganisms in the biofilm reactors had more nodes but less interactions than those in floc reactors. The large niche differences in the biofilm reactors explained the observed high microbial diversity, functional redundancy and resulting high system stability. We also observed a lower proportion of denitrifiers and higher resistance to oxygen and salinity perturbation in the biofilm reactors than the floc reactors. Our findings support our hypothesis that niche differences caused a distinct microbial structure and increased microbial ecology distribution, which has the potential to improve system efficiency and stability.

ACS Style

Yale Deng; Yunjie Ruan; Bin Ma; Michael B. Timmons; Huifeng Lu; Xiangyang Xu; Heping Zhao; Xuwang Yin. Multi-omics analysis reveals niche and fitness differences in typical denitrification microbial aggregations. Environment International 2019, 132, 105085 .

AMA Style

Yale Deng, Yunjie Ruan, Bin Ma, Michael B. Timmons, Huifeng Lu, Xiangyang Xu, Heping Zhao, Xuwang Yin. Multi-omics analysis reveals niche and fitness differences in typical denitrification microbial aggregations. Environment International. 2019; 132 ():105085.

Chicago/Turabian Style

Yale Deng; Yunjie Ruan; Bin Ma; Michael B. Timmons; Huifeng Lu; Xiangyang Xu; Heping Zhao; Xuwang Yin. 2019. "Multi-omics analysis reveals niche and fitness differences in typical denitrification microbial aggregations." Environment International 132, no. : 105085.

Article
Published: 14 September 2019 in Aquatic Ecology
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Ecological stoichiometry predicts that the extent to which herbivores are limited by nutrients depends on their metabolic demands. Nonconsumptive effects (NCEs) caused by predators have been demonstrated to trigger not only inducible defenses but also physiological stresses in prey. Stressed prey always exhibit increased metabolism and decreased nutrient deposition (e.g., nitrogen and phosphorus) as physiological stress responses to their predators. Consequently, prey exposed to predation risk should reduce demands for nutrients intake and become adaptive to nutrient limitations. In this study, results obtained from classic Brachionus–Asplanchna model revealed that Brachionus calyciflorus fed with full nutrient algal food showed energetic costs, e.g., reduced investment in asexual (lifetime fecundity) and sexual (mixis ratio) reproductions, in response to predation risk. However, reduction in sexual and asexual reproductions diminished when herbivorous Brachionus were supplied with nitrogen- or phosphorus-limited algal food. Moreover, long posterolateral spines and high posterolateral spine-body length ratio developed in either nitrogen- or phosphorus-deficient conditions containing Asplanchna kairomones, indicating an additive effect of nutrient limitation and predation risk on posterolateral spine development in Brachionus. The findings of this study highlighted predator-facilitated adaptation of prey to nutrient limitations of primary producers, which may have implications in understanding roles of NCEs in regulating trophic interactions.

ACS Style

Xuwang Yin; Haoyu Yin; Jiajia Wang; Xiangyang Xu; Yunjie Ruan. Joint effects of predation risk and food nutrient on sexual and asexual reproductions, and morphological defenses of freshwater rotifer Brachionus calyciflorus. Aquatic Ecology 2019, 54, 35 -44.

AMA Style

Xuwang Yin, Haoyu Yin, Jiajia Wang, Xiangyang Xu, Yunjie Ruan. Joint effects of predation risk and food nutrient on sexual and asexual reproductions, and morphological defenses of freshwater rotifer Brachionus calyciflorus. Aquatic Ecology. 2019; 54 (1):35-44.

Chicago/Turabian Style

Xuwang Yin; Haoyu Yin; Jiajia Wang; Xiangyang Xu; Yunjie Ruan. 2019. "Joint effects of predation risk and food nutrient on sexual and asexual reproductions, and morphological defenses of freshwater rotifer Brachionus calyciflorus." Aquatic Ecology 54, no. 1: 35-44.

Environmental biotechnology
Published: 05 April 2019 in Applied Microbiology and Biotechnology
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Biofloc technology is an efficient approach for intensive shrimp culture. However, the extent to which this process can influence the composition of intestinal microbial community is still unknown. Here, we surveyed the shrimp intestinal bacteria as well as the floc water from three biofloc systems with different stock densities. Our study revealed a similar variation trend in phylum taxonomy level between floc bacteria and gut microbiota. Microbial community varied notably in floc water from different stock densities, while a core genus with dominating relative abundance was detected in gut samples. Extensive variation was discovered in gut microbiota, but still clustered into groups according to stock density. Our results indicated that shrimp intestinal microbiota as well as bacteria aggregated in flocs assembled into distinct communities from different stock densities, and the intestinal communities were more similar with the surrounding environment as the increase of stock density and resulting high floc biomass. The high stock density changed the core gut microbiota by reducing the relative abundance of Paracoccus and increasing that of Nocardioides, which may negatively influence shrimp performance. Therefore, this study helps us to understand further bacteria and host interactions in biofloc system.

ACS Style

Yale Deng; Xiangyang Xu; Xuwang Yin; Huifeng Lu; Guangshuo Chen; Jianhai Yu; Yunjie Ruan. Effect of stock density on the microbial community in biofloc water and Pacific white shrimp (Litopenaeus vannamei) gut microbiota. Applied Microbiology and Biotechnology 2019, 103, 4241 -4252.

AMA Style

Yale Deng, Xiangyang Xu, Xuwang Yin, Huifeng Lu, Guangshuo Chen, Jianhai Yu, Yunjie Ruan. Effect of stock density on the microbial community in biofloc water and Pacific white shrimp (Litopenaeus vannamei) gut microbiota. Applied Microbiology and Biotechnology. 2019; 103 (10):4241-4252.

Chicago/Turabian Style

Yale Deng; Xiangyang Xu; Xuwang Yin; Huifeng Lu; Guangshuo Chen; Jianhai Yu; Yunjie Ruan. 2019. "Effect of stock density on the microbial community in biofloc water and Pacific white shrimp (Litopenaeus vannamei) gut microbiota." Applied Microbiology and Biotechnology 103, no. 10: 4241-4252.

Journal article
Published: 21 September 2018 in Water
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An aerobic denitrifier was isolated from a long-term poly (3-hydroxybutyrate-co-3-hydroxyvalerate) PHBV-supported denitrification reactor that operated under alternate aerobic/anoxic conditions. The strain was identified as Marinobacter hydrocarbonoclasticus RAD-2 based on 16S rRNA-sequence phylogenetic analysis. Morphology was observed by scanning electron microscopy (SEM), and phylogenetic characteristics were analyzed with the API 20NE test. Strain RAD-2 showed efficient aerobic denitrification ability when using NO3−-N or NO2−-N as its only nitrogen source, while heterotrophic nitrification was not detected. The average NO3−-N and NO2−-N removal rates were 6.47 mg/(L·h)and 6.32 mg/(L·h), respectively. Single-factor experiments indicated that a 5:10 C/N ratio, 25–40 °C temperature, and 100–150 rpm rotation speed were the optimal conditions for aerobic denitrification. Furthermore, the denitrifying gene napA had the highest expression on a transcriptional level, followed by the denitrifying genes nirS and nosZ. The norB gene was found to have significantly low expression during the experiment. Overall, great aerobic denitrification ability makes the RAD-2 strain a potential alternative in enhancing nitrate management for marine recirculating aquaculture system (RAS) practices.

ACS Style

Dedong Kong; Wenbing Li; Yale Deng; Yunjie Ruan; Guangsuo Chen; Jianhai Yu; Fucheng Lin. Denitrification-Potential Evaluation and Nitrate-Removal-Pathway Analysis of Aerobic Denitrifier Strain Marinobacter hydrocarbonoclasticus RAD-2. Water 2018, 10, 1298 .

AMA Style

Dedong Kong, Wenbing Li, Yale Deng, Yunjie Ruan, Guangsuo Chen, Jianhai Yu, Fucheng Lin. Denitrification-Potential Evaluation and Nitrate-Removal-Pathway Analysis of Aerobic Denitrifier Strain Marinobacter hydrocarbonoclasticus RAD-2. Water. 2018; 10 (10):1298.

Chicago/Turabian Style

Dedong Kong; Wenbing Li; Yale Deng; Yunjie Ruan; Guangsuo Chen; Jianhai Yu; Fucheng Lin. 2018. "Denitrification-Potential Evaluation and Nitrate-Removal-Pathway Analysis of Aerobic Denitrifier Strain Marinobacter hydrocarbonoclasticus RAD-2." Water 10, no. 10: 1298.

Journal article
Published: 02 June 2017 in AMB Express
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The interactions between environmental factors and bacterial community shift in solid-phase denitrification are crucial for optimum operation of a reactor and to achieve maximum treatment efficiency. In this study, Illumina high-throughput sequencing was applied to reveal the effects of different operational conditions on bacterial community distribution of three continuous operated poly(butylene succinate) biological denitrification reactors used for recirculating aquaculture system (RAS) wastewater treatment. The results indicated that salinity decreased OTU numbers and diversity while dissolved oxygen (DO) had no obvious influence on OTU numbers. Significant microbial community composition differences were observed among and between three denitrification reactors under varied operation conditions. This result was also demonstrated by cluster analysis (CA) and detrended correspondence analysis (DCA). Hierarchical clustering and redundancy analysis (RDA) was performed to test the relationship between environmental factors and bacterial community compositions and result indicated that salinity, DO and hydraulic retention time (HRT) were the three key factors in microbial community formation. Besides, Simplicispira was detected under all operational conditions, which worth drawing more attention for nitrate removal. Moreover, the abundance of nosZ gene and 16S rRNA were analyzed by real-time PCR, which suggested that salinity decreased the proportion of denitrifiers among whole bacterial community while DO had little influence on marine reactors. This study provides an overview of microbial community shift dynamics in solid-phase denitrification reactors when operation parameters changed and proved the feasibility to apply interval aeration for denitrification process based on microbial level, which may shed light on improving the performance of RAS treatment units.

ACS Style

Ya-Le Deng; Yun-Jie Ruan; Song-Ming Zhu; Xi-Shan Guo; Zhi-Ying Han; Zhang-Ying Ye; Gang Liu; Ming-Ming Shi. The impact of DO and salinity on microbial community in poly(butylene succinate) denitrification reactors for recirculating aquaculture system wastewater treatment. AMB Express 2017, 7, 1 -11.

AMA Style

Ya-Le Deng, Yun-Jie Ruan, Song-Ming Zhu, Xi-Shan Guo, Zhi-Ying Han, Zhang-Ying Ye, Gang Liu, Ming-Ming Shi. The impact of DO and salinity on microbial community in poly(butylene succinate) denitrification reactors for recirculating aquaculture system wastewater treatment. AMB Express. 2017; 7 (1):1-11.

Chicago/Turabian Style

Ya-Le Deng; Yun-Jie Ruan; Song-Ming Zhu; Xi-Shan Guo; Zhi-Ying Han; Zhang-Ying Ye; Gang Liu; Ming-Ming Shi. 2017. "The impact of DO and salinity on microbial community in poly(butylene succinate) denitrification reactors for recirculating aquaculture system wastewater treatment." AMB Express 7, no. 1: 1-11.

Journal article
Published: 12 April 2016 in Environmental Science and Pollution Research
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Rapid start-up of biofilter is essential for intensive marine recirculating aquaculture system (RAS) production. This study evaluated the nitrifying biofilm formation using mature biofilm as an inoculum to accelerate the process in RAS practice. The effects of inoculation ratios (0–15 %) on the reactor performance and biofilm structure were investigated. Complete nitrification was achieved rapidly in reactors with inoculated mature biofilm (even in 32 days when 15 % seeding ratio was applied). However, the growth of target biofilm on blank carrier was affected by the mature biofilm inoculated through substrate competition. The analysis of extracellular polymeric substance (EPS) and nitrification rates confirmed the divergence of biofilm cultivation among reactors. Besides, three N-acyl-homoserine lactones (AHLs) were found in the process, which might regulate the activities of biofilm. Multivariate analysis based on non-metric multidimensional scaling (nMDS) also indicated the great roles of AHLs and substrate supply which might fundamentally determine varied cultivation performance on target biofilm.

ACS Style

Songming Zhu; Jiazheng Shen; Yunjie Ruan; Xishan Guo; Zhangying Ye; Yale Deng; Mingming Shi. The effects of different seeding ratios on nitrification performance and biofilm formation in marine recirculating aquaculture system biofilter. Environmental Science and Pollution Research 2016, 23, 14540 -14548.

AMA Style

Songming Zhu, Jiazheng Shen, Yunjie Ruan, Xishan Guo, Zhangying Ye, Yale Deng, Mingming Shi. The effects of different seeding ratios on nitrification performance and biofilm formation in marine recirculating aquaculture system biofilter. Environmental Science and Pollution Research. 2016; 23 (14):14540-14548.

Chicago/Turabian Style

Songming Zhu; Jiazheng Shen; Yunjie Ruan; Xishan Guo; Zhangying Ye; Yale Deng; Mingming Shi. 2016. "The effects of different seeding ratios on nitrification performance and biofilm formation in marine recirculating aquaculture system biofilter." Environmental Science and Pollution Research 23, no. 14: 14540-14548.

Comparative study
Published: 01 September 2015 in Bioresource Technology
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Nitrate removal is essential for the sustainable operation of recirculating aquaculture system (RAS). This study evaluated the heterotrophic denitrification using poly(butylene succinate) as carbon source and biofilm carrier for RAS wastewater treatment. The effect of varied operational conditions (influent type, salinity and nitrate loading) on reactor performance and microbial community was investigated. The high denitrification rates of 0.53 ± 0.19 kg NO3(-)-N m(-3) d(-1) (salinity, 0‰) and 0.66 ± 0.12 kg NO3(-)-Nm(-3) d(-1) (salinity, 25‰) were achieved, and nitrite concentration was maintained below 1mg/L. In addition, the existence of salinity exhibited more stable nitrate removal efficiency, but caused adverse effects such as excessive effluent dissolved organic carbon (DOC) and dissimilation nitrate reduce to ammonia (DNRA) activity. The degradation of PBS was further confirmed by SEM and FTIR analysis. Illumina sequencing revealed the abundance and species changes of functional denitrification and degradation microflora which might be the primary cause of varied reactor performance.

ACS Style

Song-Ming Zhu; Ya-Le Deng; Yun-Jie Ruan; Xi-Shan Guo; Ming-Ming Shi; Jia-Zheng Shen. Biological denitrification using poly(butylene succinate) as carbon source and biofilm carrier for recirculating aquaculture system effluent treatment. Bioresource Technology 2015, 192, 603 -610.

AMA Style

Song-Ming Zhu, Ya-Le Deng, Yun-Jie Ruan, Xi-Shan Guo, Ming-Ming Shi, Jia-Zheng Shen. Biological denitrification using poly(butylene succinate) as carbon source and biofilm carrier for recirculating aquaculture system effluent treatment. Bioresource Technology. 2015; 192 ():603-610.

Chicago/Turabian Style

Song-Ming Zhu; Ya-Le Deng; Yun-Jie Ruan; Xi-Shan Guo; Ming-Ming Shi; Jia-Zheng Shen. 2015. "Biological denitrification using poly(butylene succinate) as carbon source and biofilm carrier for recirculating aquaculture system effluent treatment." Bioresource Technology 192, no. : 603-610.