Antibiotics are extensively used to treat human and animal diseases and are especially used in animal production to promote the growth performance of livestock and aquatic animals. Sulphonamides, as important drugs for aquatic animals, are often used in aquaculture to prevent and treat diseases. However, various antibiotics found in the aquatic environment exhibit varying degrees of toxicity to aquatic organisms. Antibiotics in wastewater produced in industrial and agricultural processes are not thoroughly removed by sewage treatment and are released into water, which results in varying degrees of pollution of the surrounding water environment, forcing people to pay attention towards the ecosystem. Several studies have investigated the impact of antibiotics on aquatic organisms in water environment; however, only a few studies have investigated the underlying mechanism. Antibiotics persisting in an aquatic environment for a long time can cause genotoxicity and histopathological changes in various aquatic organisms. Therefore, this paper reviews the sources of antibiotics in aquatic environment, the pollution status of sulfonamides in aquatic environment at home and abroad, and focuses on the research status of ecotoxicological effects of sulfonamides on aquatic organisms. Because there are not only antibiotic pollution, but also many other pollutants, such as heavy metals, micro plastics and other chemicals, it will be a challenge to determine the combined effects of antibiotics or other pollutants on aquatic organisms in future environmental toxicity studies.

The accumulation of antibiotics in the aquatic environment is increasingly becoming a risk to the health of aquatic animal. The purpose of this study was to investigate the acute and chronic toxicity of florfenicol (FF) to zebrafish. A 56-day chronic toxicity test followed a 96-h acute toxicity test. The chronic toxicity test was divided into five FF concentration groups: 0 mg/L (C), 5 mg/L (T5), 10 mg/L (T10), 20 mg/L (T20) and 40 mg/L (T40). Each group had five replicates, with 20 Zebrafish per replicate. The acute toxicity test results showed that the 96 h-LC50 of FF was greater than 2000 mg/L, indicating low toxicity. The exposure concentrations of FF exceeding 20 mg/L can cause oxidative damage to the liver and gill tissues of fish, leading to the accumulation of oxidative products in the tissues and severe damage to antioxidant capacity. The reactive oxygen species (ROS) generated by severe oxidative stress activates the toll like receptors (TLR) pathway, inducing inflammation in the liver and gill tissues, stimulating the upregulation of inflammatory factor expression levels, and leading to immune system disorders. FF exposure at a concentration of 5 mg/L can lead to a significant decrease in the diversity and evenness of gut microbiota. The concentration of FF in water bodies above 37.52 mg/L poses a potential risk to aquatic products.