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Oxygen is a harmful substance in many processes because it can bring out corrosion and oxidation of food. This study aimed to enhance the removal of dissolved oxygen (DO) from water by employing a novel rotor–stator reactor (RSR). The effectiveness of the nitrogen stripping coupled with vacuum degassing technique for the removal of DO from water in the RSR was investigated. The deoxygenation efficiency (η) and the mass transfer coefficient (K_La) were determined under various operating conditions for the rotational speed, liquid volumetric flow rate, gas volumetric flow rate, and vacuum degree. The nitrogen stripping coupled with vacuum degassing technique achieved values for η and K_La of 97.34% and 0.0882 s⁻¹, respectively, which are much higher than those achieved with the vacuum degassing technique alone (η = 89.95% and K_La = 0.0585 s⁻¹). A correlation to predict the K_La was established and the predicted K_La values were in agreement with the experimental values, with deviations generally within 20%. The results indicate that RSR is a promising deaerator thanks to its intensification of gas–liquid contact.