Biochar-based fertilizer amendment can improve soil properties partly due to stimulated microbial activities and growths. The karst ecosystem is prone to degradation and accounts for a large proportion of southwest China. Understanding of the response of the microbial community structure to biochar-based fertilizer application is of great significance in karst soil restoration. A field experiment located in southwest China was conducted in typical karst soil, and a high-throughput sequencing approach was used to investigate the effect of biochar-based fertilizer application on microbial community structure in karst soil. Field trials were set up for 24 months using the following treatments: control (CK), compost plus NPK fertilizer (MF), biochar (B), less biochar (half the quantity of biochar in B) plus compost and NPK fertilizer (B1MF), biochar plus compost and NPK fertilizer (BMF), and more biochar (double the quantity of biochar in B) plus compost and NPK fertilizer (B4MF). The results elucidated that BMF and B4MF treatments had higher contents of soil carbon and soil nutrients N, P, and K than the other treatments. Soil microbial abundance and diversity were significantly increased by biochar-based fertilizer amendments (BMF and B4MF), compared to CK (P < 0.05). BMF and B4MF treatments significantly increased the relative abundance of dominant microorganisms, compared to CK (P < 0.05). The difference in the composition of indicator microbes between each treated group indicated that soil amendments altered the microbial community structure. There was a strong correlation between soil properties (soil C-, N-, and P-fractions) and microbial community structure. Furthermore, network analysis revealed that the addition of biochar-based fertilizer increased the scale and complexity of the microbial co-occurrence network. To summarize, the application of biochar-based fertilizer enabled more keystone species in the soil microbial network to participate in soil carbon resource management and soil nutrient cycling, indicating that biochar-based fertilizer is beneficial for the restoration of karst-degraded soils.

Agricultural disturbance has significantly boosted soil greenhouse gas (GHG) emissions such as methane (CH₄), carbon dioxide (CO₂), and nitrous oxide (N₂O). Biochar application is a potential option for regulating soil GHG emissions. However, the effects of biochar application on soil GHG emissions are variable among different environmental conditions. In this study, a dataset based on 129 published papers was used to quantify the effect sizes of biochar application on soil GHG emissions. Overall, biochar application significantly increased soil CH₄ and CO₂ emissions by an average of 15% and 16% but decreased soil N₂O emissions by an average of 38%. The response ratio of biochar applications on soil GHG emissions was significantly different under various management strategies, biochar characteristics, and soil properties. The relative influence of biochar characteristics differed among soil GHG emissions, with the overall contribution of biochar characteristics to soil GHG emissions ranging from 29% (N₂O) to 71% (CO₂). Soil pH, the biochar C:N ratio, and the biochar application rate were the most influential variables on soil CH₄, CO₂, and N₂O emissions, respectively. With biochar application, global warming potential (impact of the emission of different greenhouse gases on their radiative forcing by agricultural practices) and the intensity of greenhouse gas emissions (emission rate of a given pollutant relative to the intensity of a specific activity) significantly decreased, and crop yield greatly increased, with an average response ratio of 23%, 41%, and 21%, respectively. Our findings provide a scientific basis for reducing soil GHG emissions and increasing crop yield through biochar application.

Bamboo has invaded native forests worldwide, and its aggressive spread by rhizomes facilitates patch expansion and the eventual replacement of adjacent forests. However, fine-scale studies of the spatial pattern and competitive relationships of bamboo in native forests are still lacking. We obtained data from nine plots in a native south subtropical rainforest in Guizhou Province, northwest China. Pair-correlation functions indicated that competition caused by bamboo expansion has not led to large-scale regular spatial distributions in bamboo forest and negative density-related dependence mechanisms regulating the spatial pattern of the native forest community. Marked correlation functions indicated small bamboo in clusters form colony patches that grow around the larger mature trees, resulting in patch expansion in the native forest community. Mark variogram functions identified significant positive spatial autocorrelation of moso bamboo caused by interactions with similar-sized trees within colony patches. This study showed that moso bamboo has colonized and expanded within the native forest community. Compared to the native forest species, the strategy of patch expansion and equal tree sizes in colony patches of moso bamboo could prevent regular distribution trend and size-asymmetric competition between nearby bamboo for the asymmetric and limited sources (i.e., light) in the forest, enhancing the persistence of moso bamboo in the native forest in our study stand located in a south subtropical rainforest in a river valley.

While rhizosphere microbiome functions in saline soils are well documented, complementary microbial strategies between pioneer and late-successional halophytes remain unexplored. Here, we used 16S rRNA sequencing and FAPROTAX functional prediction to compare the rhizosphere bacterial communities of two key halophytes—Suaeda glauca and Phragmites communis—in a reclaimed coastal wetland. The results demonstrate that both plants significantly restructured microbial communities through convergent enrichment of stress-tolerant taxa (Firmicutes, Pseudomonas, Bacillus, and Planococcus) while suppressing sulfur-oxidizing bacteria (Sulfurovum and Thiobacillus). However, they exhibited distinct microbial specialization: S. glauca uniquely enriched organic-matter-degrading taxa (Promicromonospora and Zhihengliuella) and upregulated aromatic compound degradation (2.29%) and ureolysis (0.86%) according to FAPROTAX analysis, facilitating carbon mobilization in early successional stages. Notably, P. communis selectively recruited nitrogen-cycling Serratia, with increased nitrate respiration (3.51% in P. communis vs. 0.91% in S. glauca) function, reflecting its higher nitrogen demand. Environmental factors also diverged: S. glauca’s microbiome correlated with potassium and sodium, whereas P. communis responded to phosphorus and chloride. These findings uncover distinct microbial recruitment strategies by halophytes to combat saline stress—S. glauca–P. communis synergy through microbial carbon-nitrogen coupling—offering a template for consortia design in saline soil restoration.

The present study used bibliometric methods to analyze the literature regarding the biochar effects on soil that are included in the Web of Science Core Collection database and quantified the annual number of publications in the field and distribution of publications. Using CiteSpace as a visual analytic software for the literature, the distribution of the subject categories, author collaborations, institution collaborations, international (regional) collaborations, and cocitation and keyword clustering were analyzed. The results showed the basic characteristics of the literature related to the effects of biochar on soil. Furthermore, the main research powers in this field were identified. Then, we recognized the main intellectual base in the domain of biochar effects on soil. Meanwhile, this paper revealed the research hotspots and trends of this field. Furthermore, focuses of future research in this field are discussed. The present study quantitatively and objectively describes the research status and trends of biochar effects on soil from the bibliometric perspective to promote in-depth research in this field and provide reference information for scholars in the relevant fields to refine their research directions, address specific scientific issues, and help scholars to seek/establish relevant collaborations in their fields of interests.