Unpasteurized fruit juices are prone to microbiological spoilage by bacteria, yeasts, and molds, creating a need for natural preservatives to extend shelf life without compromising quality. Pomegranate peel extract (PPE) exhibits antimicrobial activity primarily against pathogenic microorganisms; however, its effect on spoilage microorganisms in fruit juices has not been previously studied. This work aimed to compare the microbiological stability of unpasteurized apple juice when adding non-encapsulated PPE or PPE microparticles produced by spray drying with inulin (PPE-IN), inulin combined with sodium alginate (PPE-(IN+SA)), or inulin combined with carrageenan (PPE-(IN+CR)) as encapsulating agents. All microparticle systems showed high encapsulation efficiency (>90%), with PPE-IN reaching 94.08%. For the stability study, PPE microparticles (0.018 to 0.023 g/mL) or PPE (0.009 g/mL) were added to 70 mL of juice and stored at 4 °C for 9 days. MAB, molds, and yeasts were then quantified. At 3, 6, and 9 days, the J+PPE-(IN+CR) treatment showed the lowest MAB, molds, and yeast counts in relation to the non-encapsulated PPE and the other microparticle system. These results indicate that PPE-(IN+CR) microparticles provide superior microbiological stability of apple juice up to day 9, suggesting that the combination of encapsulating agents (IN+CR) could control the release of PPE into the juice.

Good Laboratory Practices (GLP) in cell culture are essential to ensure the quality and reproducibility of results in scientific and biomedical research. This review aimed to comprehensively analyze the regulations and standards related to GLP in the management of cell cultures, emphasizing their importance for the quality and reliability of scientific findings. A literature review was conducted, encompassing 33 articles published between 1994 and 2023 in academically relevant databases such as Scopus, ProQuest, ResearchGate, ScienceDirect, Springer, among others. Search terms included "Good Laboratory Practices," "GLP," "regulations," and "cell cultures," with specific selection criteria applied. Extracted data covered regulatory frameworks, governing bodies, and areas of application. The findings highlight that guidelines issued by organizations such as the Organisation for Economic Co-operation and Development (OECD), the U.S. Food and Drug Administration (FDA), and the European Medicines Agency (EMA) are essential for the proper implementation of GLP in laboratories working with cell cultures. Furthermore, critical aspects such as precise documentation of procedures, continuous staff training, and implementation of quality and sterility control measures are addressed. The review concludes that the rigorous application of GLP is crucial for ensuring reproducibility, quality, and safety of results, as well as for compliance with regulatory standards—thereby strengthening scientific integrity and fostering advances in biotechnology and biomedicine.

Good Laboratory Practices (GLP) in cell culture are essential to ensure the quality and reproducibility of results in scientific and biomedical research. This review aimed to comprehensively analyze the regulations and standards related to GLP in the management of cell cultures, emphasizing their importance for the quality and reliability of scientific findings. A literature review was conducted, encompassing 33 articles published between 1994 and 2023 in academically relevant databases such as Scopus, ProQuest, ResearchGate, ScienceDirect, Springer, among others. Search terms included "Good Laboratory Practices," "GLP," "regulations," and "cell cultures," with specific selection criteria applied. Extracted data covered regulatory frameworks, governing bodies, and areas of application. The findings highlight that guidelines issued by organizations such as the Organisation for Economic Co-operation and Development (OECD), the U.S. Food and Drug Administration (FDA), and the European Medicines Agency (EMA) are essential for the proper implementation of GLP in laboratories working with cell cultures. Furthermore, critical aspects such as precise documentation of procedures, continuous staff training, and implementation of quality and sterility control measures are addressed. The review concludes that the rigorous application of GLP is crucial for ensuring reproducibility, quality, and safety of results, as well as for compliance with regulatory standards—thereby strengthening scientific integrity and fostering advances in biotechnology and biomedicine.