Skin tissue engineering has gained significant attention as a promising alternative to traditional treatments for skin injuries. In this study, we developed 3D hydrogel-based scaffolds, Alginate, incorporating different concentrations of Curcumin and evaluated their properties, including morphology, swelling behavior, weight loss, as well as hemo- and cytocompatibility. Furthermore, we investigated the therapeutic potential of Alginate hydrogel containing different amounts of Curcumin using an in vitro wound healing model. The prepared hydrogels exhibited remarkable characteristics, SEM showed that the pore size of hydrogels was 134.64 μm with interconnected pores, making it conducive for cellular infiltration and nutrient exchange. Moreover, hydrogels demonstrated excellent biodegradability, losing 63.5% of its weight over 14 days. In addition, the prepared hydrogels had a stable release of curcumin for 3 days. The results also show the hemocompatibility of prepared hydrogels and a low amount of blood clotting. To assess the efficacy of the developed hydrogels, 3T3 fibroblast growth was examined during various incubation times. The results indicated that the inclusion of Curcumin at a concentration of 0.1 mg/mL positively influenced cellular behavior. The animal study showed that Alginate hydrogel containing 0.1 mg/mL curcumin had high wound closure(more than 80%) after 14 days. In addition, it showed up-regulation of essential wound healing genes, including TGFβ1 and VEGF, promoting tissue repair and angiogenesis. Furthermore, the treated group exhibited down-regulation of MMP9 gene expression, indicating a reduction in matrix degradation and inflammation. The observed cellular responses and gene expression changes substantiate the therapeutic efficacy of prepared hydrogels. Consequently, our study showed the healing effect of alginate-based hydrogel containing Curcumin on skin injuries.

This study focuses on the development of a 3-dimensional porous scaffold using Polycaprolactone/Gelatin/Nanoclay (PCL/GNF/NC) for bone tissue engineering. The scaffold incorporates varying dosages of silybin (Sil) through a mixture of electrospinning and thermal-induced phase separation (TIPS) techniques. Assessments of surface shape, porosity, compressive strength, water contact angle, degradation rate, releasing profile, hemolysis, and cell proliferation were among the investigations carried out to appraise the manufactured scaffolds. In vivo evaluation utilized a rat calvaria defect model, with histological analysis employed to assess the results. The scaffolds exhibited porosity within the range of 70-90%, and those containing silybin demonstrated lower compressive strength and contact angle, along with a higher degradation rate compared to those without silybin. Release experiments revealed a 61.09% release of silybin after 28 days. In both in vivo and in vitro assessments, the PCL/GNF/NC/Sil1% scaffold displayed superior cell proliferation and bone healing properties compared to other groups. These findings suggest the potential efficacy of silybin in bone defect treatment, warranting further investigation in future research.