Lakes are important to the healthy functioning of the urban ecosystem. The urban lakes in Wuhan, China, which is known as ‘city of hundreds of lakes’, are facing substantial threats mainly due to rapid urbanization. This paper focused on detecting the spatial and temporal change of urban lakes in Wuhan, using a long time series of Landsat and HJ-1A remotely sensed data from 1987 to 2016. The permanent disappearance and seasonal fluctuation of 28 main urban lakes were analysed, and their relationships with climatic change and human activities were discussed. The results show that most lakes in Wuhan had shrunk over the past 30 years resulting in a permanent change from water to land. The shrinkage was also most apparent in the central region of the city. Seasonal fluctuations of lake area were evident for most lakes but the relative important driving variable of lake area change varied between sub-periods of time for different lakes. The explanatory power of impervious surface to five-year permanent water change is 91.75%, suggesting that urbanization – as increasing impervious surface – had led to the shrinkage of urban lakes in Wuhan. In all, 128.28 km² five-year permanent water disappeared from 1987 to 2016.