Michael G. Schrlau

Dr. Michael G. Schrlau


Research Focus & Metrics

Academic Career Length

20Years (2007-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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20 July 2009 in Journal of Cell Biology
Verified
Journal Article
Open access
PDF file
EBEugen Brailoiu
Eugen Brailoiu
DCDev Churamani
Dev Churamani
Xinjiang CaiXinjiang Cai
Xinjiang Cai
MSMichael G. Schrlau
Michael G. Schrlau
GBG. Cristina Brailoiu
G. Cristina Brailoiu
SPSandip Patel
Sandip Patel
Nicotinic acid adenine dinucleotide phosphate (NAADP) is a widespread and potent calcium-mobilizing messenger that is highly unusual in activating calcium channels located on acidic stores. However, the molecular identity of the target protein is unclear. In this study, we show that the previously uncharacterized human two-pore channels (TPC1 and TPC2) are endolysosomal proteins, that NAADP-mediated calcium signals are enhanced by overexpression of TPC1 and attenuated after knockdown of TPC1, and that mutation of a single highly conserved residue within a putative pore region abrogated calcium release by NAADP. Thus, TPC1 is critical for NAADP action and is likely the long sought after target channel for NAADP.

4 February 2009 in ACS Nano
Verified
Journal Article
Closed access
MSMichael G. Schrlau
Michael G. Schrlau
NDNae J. Dun
Nae J. Dun
HBHaim H Bau
Haim H Bau

29 November 2007 in Nanotechnology
Verified
Journal Article
Closed access
MSMichael G. Schrlau
Michael G. Schrlau
EFErica M Falls
Erica M Falls
BZBarry L Ziober
Barry L Ziober
HBHaim H Bau
Haim H Bau
We developed integrated, carbon-based pipettes with nanoscale dimensions (CNP) that can probe cells with minimal intrusion, inject fluids into the cells, and concurrently carry out electrical measurements. Our manufacturing technique does not require cumbersome nanoassembly and is amenable to mass production. Using CNPs, we demonstrate the injection of reagents into cells with minimal intrusion and without inhibiting cell growth.

28 December 2010 in Small
Verified
Journal Article
Closed access
PDF file
JNJun Jie Niu
Jun Jie Niu
MSMichael G. Schrlau
Michael G. Schrlau
GFGary Friedman
Gary Friedman
Yury GogotsiYury Gogotsi
Yury Gogotsi

5 April 2016 in Small
Verified
Journal Article
Closed access
PDF file
MGMasoud Golshadi
Masoud Golshadi
LWLeslie K. Wright
Leslie K. Wright
IDIan M. Dickerson
Ian M. Dickerson
MSMichael G. Schrlau
Michael G. Schrlau

2 July 2008 in Nanotechnology
Verified
Journal Article
Closed access
MSMichael G. Schrlau
Michael G. Schrlau
EBEugen Brailoiu
Eugen Brailoiu
SPSandip Patel
Sandip Patel
Yury GogotsiYury Gogotsi
Yury Gogotsi
NDNae J Dun
Nae J Dun
HBHaim H Bau
Haim H Bau
Carbon-based nanoprobes are attractive for minimally invasive cell interrogation but their application in cell physiology has thus far been limited. We have developed carbon nanopipettes (CNPs) with nanoscopic tips and used them to inject calcium-mobilizing messengers into cells without compromising cell viability. We identify pathways sensitive to cyclic adenosine diphosphate ribose (cADPr) and nicotinic acid adenine dinucleotide phosphate (NAADP) in breast carcinoma cells. Our findings demonstrate the superior utility of CNPs for intracellular delivery of impermeant molecules and, more generally, for cell physiology studies. The CNPs do not appear to cause any lasting damage to cells. Their advantages over commonly used glass pipettes include smaller size, breakage and clogging resistance, and potential for multifunctionality such as in concurrent injection and electrical measurements.