A method to determine the spring constant of a rectangular atomic force microscope cantilever is proposed that relies solely on the measurement of the resonant frequency and quality factor of the cantilever in fluid (typically air), and knowledge of its plan view dimensions. This method gives very good accuracy and improves upon the previous formulation by Sader et al. [Rev. Sci. Instrum. 66, 3789 (1995)] which, unlike the present method, requires knowledge of both the cantilever density and thickness.

Detailed measurements of the frequency responses of a series of rectangular atomic force microscope (AFM) cantilever beams, immersed in a range of fluids, have been performed to test the validity and accuracy of the recent theoretical model of Sader [J. Appl. Phys. 84, 64 (1998)]. This theoretical model gives the frequency response of a cantilever beam, that is immersed in a viscous fluid and excited by an arbitrary driving force. Very good agreement between experimental measurements and theoretical calculations is found for all fluids considered. Furthermore, a critical assessment of the well-known inviscid model is presented, which demonstrates that this model is not applicable to AFM cantilever beams in general.