Strains HUAS TT3 ^T and HUAS TT20 ^T were isolated from rhizosphere soil samples of Cyclosorus acuminatus collected in Qianjiang, Hubei Province, China. Sequence analyses of the 16S rRNA gene of strains HUAS TT3 ^T and HUAS TT20 ^T indicated that they belonged to the genus Streptomyces . Strain HUAS TT3 ^T exhibited the highest sequence similarity of 99.79% to Streptomyces racemochromogenes JCM 4407 ^T , Streptomyces polychromogenes JCM 4505 ^T , and Streptomyces yangpuensis fd2-11 tb ^T , while strain HUAS TT20 ^T exhibited the highest sequence similarity of 98.76% to Streptomyces barringtoniae JA03 ^T . Phylogenetic trees constructed using the 16S rRNA gene, five housekeeping genes ( atpD , gyrB , recA , rpoB, and trpB ), and whole genome sequences indicated that strain HUAS TT3 ^T is most closely related to Streptomyces toxytricini NRRL B-5426 ^T and Streptomyces globosus JCM 13859 ^T , while strain HUAS TT20 ^T is most closely related to S. barringtoniae JA03 ^T . However, the average nucleotide identity based on MUMmer (ANIm) and digital DNA–DNA hybridization (dDDH) values were as follows: between HUAS TT3 ^T and S. toxytricini JCM 4421 ^T , the values were 88.08 and 30.10%; between HUAS TT3 ^T and S. globosus JCM 13859 ^T , the values were 88.08 and 30.30%; between HUAS TT20 ^T and S. barringtoniae JA03 ^T , the values were 82.71 and 28.20%. These values were considerably lower than the ANIm and dDDH values for the species boundaries for Streptomyces . Moreover, the differential comparisons of phenotypic, cultural, and chemotaxonomic characteristics between HUAS TT3 ^T and S. toxytricini CGMCC 4.1734 ^T , HUAS TT3 ^T and S. globosus JCM 13859 ^T , and HUAS TT20 ^T and S. barringtoniae LMG 32415 ^T also supported this conclusion. As mentioned above, we conclude that strains HUAS TT3 ^T and HUAS TT20 ^T represent two novel Streptomyces species, for which the names Streptomyces pallidus sp. nov. HUAS TT3 ^T (MCCC 1K10084 ^T = JCM 38172 ^T ) and Streptomyces qianjiangensis sp. nov. HUAS TT20 ^T (=MCCC 1K10090 ^T = JCM 38175 ^T ) are proposed.