Gas vesicles (GVs) are hollow protein nanostructures derived from microorganisms and show significant potential for ultrasound imaging applications. However, the direct production of gas vesicles (GVs) from their native hosts faces several limitations: poor contrast imaging performance, insufficient yield, and high costs associated with extraction and purification. These challenges heavily hinder their clinical translation and application. The heterologous expression of GV genes varies significantly among different chassis strains due to their distinct intracellular environments, which ultimately affects GV performance and yield. Therefore, it is crucial to select an appropriate chassis cell that can produce GVs with excellent imaging performance. In this study, the GV gene cluster from Serratia sp. ATCC 39006 was heterologously expressed in five different bacterial chassis strains: Escherichia coli BL21 (AI), Escherichia coli K-12 MG1655, Escherichia coli Nissle 1917, Salmonella YB1, and Vibrio natriegens. By systematically comparing the yield, particle morphology, and ultrasound imaging performance of GVs produced by these strains, we elucidated the impact of chassis cells on GV synthesis and function. This work provides experimental evidence and theoretical support for screening robust GV-producing strains and facilitates future biomedical applications of GVs.