Background: Metabolism including anabolism and catabolism is a prerequisite phenomenon for all living organisms. Anabolism refers to the synthesis of the entire compound needed by a species. Catabolism refers to the breakdown of molecules to obtain energy. Many metabolic pathways are undisclosed and many organism-specific enzymes involved in metabolism are misplaced. When predicting a specific metabolic pathway of a microorganism, the first and foremost steps is to explore available online databases. Among many online databases, KEGG and MetaCyc pathway databases were used to deduce trehalose metabolic network for bacteria Variovorax sp. PAMC28711. Trehalose, a disaccharide, is used by the microorganism as an alternative carbon source. Results: While using KEGG and MetaCyc databases, we found that the KEGG pathway database had one missing enzyme (maltooligosyl-trehalose synthase, EC 5.4.99.15). The MetaCyc pathway database also had some enzymes. However, when we used RAST to annotate the entire genome of Variovorax sp. PAMC28711, we found that all enzymes that were missing in KEGG and MetaCyc databases were involved in the trehalose metabolic pathway. Conclusions: Findings of this study shed light on bioinformatics tools and raise awareness among researchers about the importance of conducting detailed investigation before proceeding with any further work. While such comparison for databases such as KEGG and MetaCyc has been done before, it has never been done with a specific microbial pathway. Such studies are useful for future improvement of bioinformatics tools to reduce limitations.

Recognizing the offensive, abusive, and profanity of multimedia content on the web has been a challenge to keep the web environment for user’s freedom of speech. As profanity filtering function has been developed and applied in text, audio, and video context in platforms such as social media, entertainment, and education, the number of methods to trick the web-based application also has been increased and became a new issue to be solved. Compared to commonly developed toxic content detection systems that use lexicon and keyword-based detection, this work tries to embrace a different approach by the meaning of the sentence. Meaning representation is a way to grasp the meaning of linguistic input. This work proposed a data-driven approach utilizing Abstract meaning Representation to extract the meaning of the online text content into a convolutional neural network to detect level profanity. This work implements the proposed model in two kinds of datasets from the Offensive Language Identification Dataset and other datasets from the Offensive Hate dataset merged with the Twitter Sentiment Analysis dataset. The results indicate that the proposed model performs effectively, and can achieve a satisfactory accuracy in recognizing the level of online text content toxicity.

Life-Log is a term used for the daily monitoring of health conditions and recognizing anomalies from data generated by sensor devices. The development of smart sensors enables collection of health data, which can be considered as a solution to risks associated with personal healthcare by raising awareness regarding health conditions and wellness. Therefore, Life-Log analysis methods are important for real-life monitoring and anomaly detection. This study proposes a method for the improvement and combination of previous methods and techniques in similar fields to detect anomalies in health log data generated by various sensors. Recurrent neural networks with long short-term memory units are used for analyzing the Life-Log data. The results indicate that the proposed model performs more effectively than conventional health data analysis methods, and the proposed approach can yield a satisfactory accuracy in anomaly detection.