| The conversion of regular pumpkin seed oil into a nanotechnology-driven composition involves encapsulating Lidocaine within the phytosome structure of the oil. This process aims to bolster its physical resilience and therapeutic advantages while mitigating potential adverse reactions. The central objective of this research centers on assessing the curative impacts of the innovative Nano phytosome pumpkin-lidocaine gel in the context of third-degree burn wound healing. The reformulation of standard pumpkin seed oil involved the encapsulation of conventional Lidocaine within a phytosome structure, resulting in the formation of nanoparticles. This modification was undertaken to enhance both the physical stability of the oil and the therapeutic properties of pumpkin seed oil, while simultaneously mitigating the potential side effects associated with Lidocaine. Evaluation of the therapeutic effect of Nano phytosome pumpkin-lidocaine gel on the healing of third burn wounds. Twenty-five,10 to 18-week- old ,white male and female adult rabbits weighting 2.5-3 kg divided to5 groups (n=5) as following negative control group, positive control group, ordinary pumpkin seed oil group, Nano phytosome pumpkin-lidocaine 100% gel group, and Nano phytosome pumpkin 100% gel group. The Nano phytosome pumpkin-lidocaine gel at a concentration of 100% and the Nano phytosome pumpkin gel at a concentration of 100% showed a significant entrapment % and loaded efficiency%, entrapment % were 95.2 ± 9.41, and 87.32 ± 8.1 respectively, while loaded efficiency% were 86.84 ± 7.77, and 79.40± 3.72 respectively. As well as, both the Nano phytosome pumpkin-lidocaine gel at a concentration of 100% and the Nano phytosome pumpkin gel at a concentration of 100% revealed a significant closure rate of 0.216±0.05, and 0.215 ±0.03 mm/day respectively, compared to ordinary pumpkin seed oil treated group, and both positive and negative control group. From this study concluded the pumpkin seed oil loaded with Nano phytosome pumpkin-lidocaine demonstrated good physical stability, reducing the crystal nature of active extract substances, with good entrapment% and loaded efficiency %, and improving the wound healing of burn wound.