This study focused on loin quality in Italian heavy pigs intended for the production of PDOs (Protected Designation of Origin) products, and investigated the pre-slaughter factors which negatively affect the quality of fresh meat. Data were collected on 44 shipments (loads) of pigs. Shipments were carried out under commercial conditions. Several pre-slaughter parameters were recorded within the entire process (on-farm, during transport, and at the slaughterhouse). On a subset of pigs (10 animals from every load, N = 440), serum cortisol and creatine kinase were measured and loin samples were analyzed for pH, instrumental color, drip loss, cooking loss, shear force, and sensory quality. Cluster analysis of the instrumentally-assessed meat quality parameters allowed the categorization of the shipments into two clusters: lower quality (LQ) and higher quality (HQ). Our results showed that the factors with significant differences between the two clusters were journey duration, ambient temperature, distance traveled, and irregular behaviors (slipping, falling, and overlapping) at unloading (all greater in LQ, p < 0.05). The pre-slaughter conditions associated with lower loin quality were ambient temperatures above 22 °C, distance traveled above 26 km, travel duration between 38–66 min, more than 5.9% of animals showing irregular behaviors at unloading.
The adoption of innovative processes, such as the automatic milking system (AMS), in the production of typical PDO cheeses, such as Parmigiano Reggiano, needs to be evaluated and tested, to verify its influence on milk quality and the typicality of the product. The present research was aimed to study the effect of the introduction of the AMS on the cheesemaking characteristics of the milk and the ripening process and the sensory properties of the Parmigiano Reggiano PDO cheese. Six cheesemaking trials were performed and, in each trial, two different separated pools of milk were submitted to the cheesemaking process in parallel, one from AMS and the other from a traditional milking parlor (TMP). AMS milk, in comparison with TMP one, showed higher content of lactose and calcium and lower contents of somatic cells, thermophilic lactic acid bacteria and chloride. Nevertheless, these changes were too small to influence the efficiency of the cheesemaking process and the cheese composition and its sensory profile. Moreover, cheeses made with AMS milk fully complied with the sensory characteristics of Parmigiano Reggiano cheese PDO, as defined by the PDO rules.
Scotta is an underutilized whey by-product of ricotta making. In this study, we investigated the utilization of scotta in beer production. To understand the quality of regional scotta, samples from eight Sicilian ricotta makers were analyzed for pH, total protein, fat, lactose, titratable acidity, % salt, total plate count, lactic acid bacteria counts, and minerals. Overall, the samples had low amounts of residual protein and fat. The average lactose content was 4.81 g/100 mL ± 0.52 g, with a pH of 6.12 ± 0.17 and a salt content of 1.05% ± 0.24. The majority of lactic acid bacteria counts were below the limit of detection. The total plate counts were more variable, ranging between 102 to 103 CFU/mL, suggesting occasional post-processing contamination during handling. Scotta was then used to replace some of the water and sugar in the production of two beer styles: (i) a Gose, a salty and acidified German beer style, and (ii) a sweet milk stout. A trained panel used for sensory analysis found that these prototypes fit within the sensory profiles of commercial beers of these styles. This work highlights opportunities to upcycle dairy by-products into novel fermented beverages that would be appealing to consumers.
Brine soaking is one of the most important steps in the production of Parmigiano Reggiano cheese, since it determines the amount of salt in the final product. Reduction in salt in Parmigiano Reggiano cheese might be important for improving its nutritional profile, but it could affect the manufacturing processes by altering proteolysis and consequently the product quality. In this study, for the first time, salt reduction was explored at the industrial level on real cheese samples manufactured in a local dairy. In particular, 20 wheels were produced with conventional (18 days, 10 wheels) and shorter (12 days, 10 wheels) brining steps. In every group, wheels were studied at two different ripening times, 15 and 30 months. A shorter brining time resulted in an average 12% decrease in salt content. A full characterization of free amino acids and peptides was performed by LC-MS on all samples. Free amino acids and peptides, as expected, increased with ripening, due to proteolysis, with samples having low salt content showing a slightly faster increase when compared to standard ones, hinting to a slightly accelerated proteolytic process. Nonetheless, low-salt and conventional cheeses shared similar sensory profiles at both ripening times.
Consumer demand for high-quality products, combined with expectations for more sustainable production systems and animal welfare, is driving major changes in livestock farming practices. It is known that space allowance plays a central role in pig welfare, promoting resting and reducing the incidence of injuries and stress-related behaviors; however, there is little scientific evidence on the effect that available space has on the carcass and meat quality. In this study, space allowances were compared, in both an indoor conventional system (1.15, 1.9 and 3 m2/pig) and an indoor organic system with outdoor access (1.4 + 1, 2.6 + 2 and 3.9 + 3 m2/pig). The increase in space available for pigs had no effect on pig performance, carcass and meat quality characteristics, such as pH, drip and cooking loss. However, lowering stocking density in the conventional indoor housing system improved meat tenderness, as assessed by the Slice Shear Force test, while no difference was found between meat tenderness in organic pigs raised with three different stocking densities. Increased space allowance per pig reduced n-3 fatty acids in pig loins from both housing systems and n-6 fatty acids and PUFAs in loins from pigs reared in the organic housing system with both indoor and outdoor space.
European pork production pursues traceability and authenticity to ensure animal welfare, food safety, and support products with geographical indications. This study reports a European survey integrating stable isotope ratios (δ13C, δ15N, δ34S, δ18O, δ2H) and multi-element profiling using IRMS and ICP-MS, on 612 samples collected across Denmark, Poland, Italy, and Spain, with diverse production systems, breeds, feeding, and slaughter ages. Geographical and climatic gradients influenced δ2H and δ18O, which ranged from −111‰ to −89‰ in samples from Denmark and Spain and from 13.3‰ to 16.0‰ in samples from Italy and Spain, respectively. In selected farms, δ13C ranged from −22.7‰ to −17.0‰ depending on diet composition based on C3 and C4 plants. The wide variability in pig management practices suggested that δ15N (2.50 ÷ 4.96‰) increased with slaughter age and was positively correlated with Fe (3.38 ÷ 8.39 mg/kg) and Zn (9.39 ÷ 23.6 mg/kg). Most mineral components were mainly driven by feed formulation and supplementation. Principal component analysis (PCA) showed that samples were grouped based on their origin and husbandry system, confirming the key role of isotopic and elemental markers for the development of a database supporting the pork supply chains across Europe.