Bushbuckridge, South Africa, remains a challenging setting for malaria elimination due to residual transmission and diverse Anopheles populations. This study assessed Anopheles species diversity and insecticide susceptibility across 29 localities between 2022 and 2024. Morphological and PCR-based identification revealed 18 taxa, primarily Anopheles pretoriensis, An. quadriannulatus, and An. rufipes, and the primary vector An. arabiensis accounted for 7.1% of collections. Two putative An. arabiensis/An. quadriannulatus hybrids were detected, warranting further genomic investigation. While vector populations were largely susceptible to DDT, deltamethrin, and pirimiphos-methyl, reduced DDT susceptibility was observed in the An. funestus group; however, due to small sample sizes, this result remains inconclusive. These findings underscore the necessity of sustained, integrated vector surveillance to inform strategic control in complex transmission landscapes.