Background: The current COVID-19 pandemic has demonstrated the enormous importance of maintaining good hygienic conditions in everyday indoor environments for the prevention of infectious diseases. This includes sanitization methods capable of significantly reducing the microbial load in the air and on surfaces. However, in line with the ecological transition, alternative systems for environmental sanitization with reduced environmental impact are urgently needed. The photocatalytic reaction using UV-C light-emitting diode (UV-C LED) lamps with short wavelengths, especially in the range of 200–280 nanometers (nm), can significantly reduce the microbial load, safeguarding the environment thanks to reduced energy consumption. The objective of this review is to describe the latest innovations in the use of UV-C LED technology in the sanitization of indoor environments, reporting the fundamental principles on which its activity relies. Methods: Two databases (PubMed, Web of Science), were searched, following PRISMA guidelines. Results: A total of 1348 publications were identified, of which 379 were assessed in detail and, of these, 16 were included in the review. Conclusions: This literature review highlighted that UV-C LEDs irradiation represents a valid, eco-sustainable sanitization method that could be exploited as an alternative to chemical compounds to contain indoor microbiological pollution in living and working environments.

In this study, we used both a WGS and an in vitro approach to study the virulence potential of nine Listeria monocytogenes (Lm) strains belonging to genetic clusters persisting in a meat processing plant in Central Italy. The studied clusters belonged to CC1-ST1, CC9-ST9, and CC218-ST2801. All the CC1 and CC218 strains presented the same accessory virulence genes (LIPI-3, gltA, gltB, and aut_IVb). CC1 and CC9 strains presented a gene profile similarity of 22.6% as well as CC9 and CC218 isolates. CC1 and CC218 showed a similarity of 45.2% of the same virulence profile. The hypervirulent strains of lineage I (CC1 and CC218) presented a greater ability to adhere and invade Caco-2 cells than hypovirulent ones (CC9). CC1 strains were significantly more adhesive and invasive compared with CC9 and CC218 strains, although these last CCs presented the same accessory virulence genes. No statistically significant difference was found comparing CC218 with CC9 strains. This study provided for the first time data on the in vitro adhesiveness and invasiveness of CC218-ST2801 and added more data on the virulence characteristics of CC1 and CC9. What we observed confirmed that the ability of Lm to adhere to and invade human cells in vitro is not always decipherable from its virulence gene profile.

The beneficial effects that physical activity gives to our bodies are well known, but they could be compromised by reduced indoor air quality (IAQ) in confined sports environments. Within the latter, indoor air often may host high levels of biological pollutants (bacteria, viruses and moulds). The use of UV-C light-emitting diode (UV-C LED) technology can significantly reduce the airborne microbial load, giving many advantages both on a hygienic and ecological level thanks to their reduced environmental impact. In this study, a new eco-friendly technology, named ‘CSA System technology’ (international patent: US20220268458A1), was integrated into an innovative continuous sanitation air (CSA) device. A prototype called CSA System S14000 was installed in a university sport pressostatic structure, and its sanitation efficacy and efficiency were assessed. The prototype demonstrated high efficacy in eliminating airborne microorganisms, both in efficacy tests (inactivation rate around 99.92 ± 0.07%, log reduction of 3.22 ± 0.39) and in on-site environmental tests (inactivation rate around 70.7 ± 3.6% and 72.4 ± 3.3% for airborne bacteria and moulds, respectively). Based on the results, the installation of the tested device in indoor sports environments appears to be a promising solution to guarantee efficient and healthy sanitation for athletes during sporting activity.

The development of antimicrobial resistance (AMR) and the emergence of multiresistant pathogens represent a growing global threat to both human and animal health. Beyond the excessive and improper use of antimicrobials in human medicine, irrational use in veterinary medicine, agriculture, and aquaculture significantly contributes to the selection and spread of resistant microorganisms, which can enter the food chain and reach humans through food consumption or handling. Based on results from a recent meta-analysis, the prevalence of antimicrobial-resistant foodborne pathogens in food samples exceeds 10%. The veterinary sector is of particular concern, as a large proportion of antimicrobials are used in animal production, generating strong selective pressure and favoring the dissemination of AMR along the food chain. In an increasingly interconnected global context, resistant pathogens and resistance determinants can disseminate rapidly across sectors and national borders, making strategies confined to a single sector insufficient; therefore, effectively addressing AMR requires a One Health approach encompassing the human, veterinary, and environmental domains. Key mitigation strategies include strengthening antimicrobial stewardship programs, also in animal production, reducing routine prophylactic use of antimicrobials, and improving surveillance, coordinated across sectors and, where possible, further supported by advanced technologies such as artificial intelligence and machine learning. Further efforts are also needed to improve microbiological diagnostics, particularly through rapid and molecular methods, to support timely, targeted therapies and reduce inappropriate empirical treatments. In parallel, investment in new therapeutic options, including innovative molecules, drug combinations, and alternative approaches, remains crucial to effectively countering the growing burden of antimicrobial resistance.

This study investigates whether a university teaching complex equipped with CSA S600 continuous air purification and sanitation units can maintain indoor air quality (IAQ) within recommended thresholds under real occupancy conditions and evaluates the impact of renovation works on IAQ. The work provides the first real-world assessment of the CSA S600 integrated monitoring system in an academic environment. CO₂, PM_2.5, PM₁₀ and VOCs were continuously measured over three months; moreover, indoor PM₁₀ values were compared with outdoor data from the regional monitoring network. Indoor CO₂ generally remained below 800 ppm, with short peaks of 1000–1500 ppm during high occupancy. PM_2.5 and PM₁₀ consistently stayed below the latest WHO guidelines, showing uniform recurring temporal patterns overtime; furthermore, indoor PM₁₀ showed limited coupling with outdoor trends, indicating the predominance of internal sources and ventilation dynamics. After renovation of the main Lecture Hall, particulate levels remained low, while VOCs showed a modest increase attributable to new materials. Overall, the findings demonstrate that the CSA S600 system effectively supports healthy IAQ in educational settings and that continuous monitoring is essential for managing occupancy-driven fluctuations and assessing the effects of structural interventions.