Platelet rich plasma samples from 50 healthy horses of five different breeds (Thoroughbreds - TB, Brazilian Criollo Horses - BCH, Brazilian Sport Horses - BSH, Miniature Horses - MH and Crossbred Horses - CB), were investigated as to breed, age, and gender effect for platelet concentration. Moreover, a score for physical analysis was established to correlate PRP physical aspect with platelet count. Platelet count was performed by an automatic hematology analyzer and by manual count. PRP physical analysis was based on color, aspect, and capacity to separate blood components. MH showed significant higher platelet concentration than BSH (p<0.05), while the other breed comparisons showed no significant difference. There was no significant difference for gender but there was a weak correlation of age with PRP platelet concentrations (rs = -0.24). Most of the PRP presented yellow color, the separation of blood components showed no correlation, but the aspect showed a moderate correlation (rs = 0.30) with platelet count. Results suggest that PRP platelet concentration can be influenced by intrinsic factors such as breed. Additionally, the analysis of PRP aspect can help to evaluate the quality of the product when there is no access to platelet counts.

Anais do Simpósio Internacional do Cavalo Atleta (SIMCAV) (978-65-5941-211-2) - Transsecção E Elevação Radical De Periósteo Em Um Potro Com Deformidade Angular Na Diáfise Do Terceiro Metatarsiano

One of the main obstacles of equine breeding is the presence of subfertile mares in the herd as they reduce pregnancy rates during the breeding season. Bacteriological exams made by collection of endometrial swabs is a reliable method of detecting the subfertility’s origin and diagnosing uterine infections. In this retrospective study, 42 uterine swabs obtained from problem-breeding mares at the northwest and central region of Rio Grande do Sul - Brazil, were analyzed from 2016 to 2019. After microbiological culture, 30 swabs were positive for the presence of 37 microorganisms that cause endometritis. Escherichia coli (45.94%), followed by coagulase-negative (CNS) Staphylococcus sp. (18.92%), Klebsiella aerogenes (10.81%), Corynebacterium sp. (5.41%) and Streptococcus sp. (5.41%) were the most prevalent isolated microorganisms. The antimicrobials susceptibility tests revealed that ciprofloxacin, enrofloxacin and norfloxacin were the antibiotics with the greatest action in vitro against isolated microorganisms. In addition, neither Gram positive nor negative bacteria showed more resistance when compared to each other (p=0.239).

BackgroundIntra-articular local anesthetics are widely used for diagnostic analgesia in horses, but their effects on lameness and the synovial environment may differ between drugs.Aims/objectiveso compare clinical efficacy and synovial fluid biochemical response after intra-articular administration of mepivacaine and ropivacaine in horses.MethodsIn a prospective study, 24 middle carpal joints from 12 clinically healthy horses were evaluated for synovial fluid total protein and lactate dehydrogenase activity before and 24 h after administration of mepivacaine 3% (100 mg) or ropivacaine 1% (40 mg), each diluted with 0.9% saline to a final volume of 5 mL. The same doses were used in a second randomized crossover experiment involving eight horses with naturally occurring tarsal joint-related lameness, with clinical efficacy assessed by objective inertial-sensor gait analysis from baseline to 240 min.ResultsObjective lameness values changed over time (P < 0.001), with no treatment effect (P = 0.620) or treatment-by-time interaction (P = 0.409). Mean clinical improvement approached 70% for both treatments at 10 min. At 240 min, improvement was approximately 86% after ropivacaine and 46% after mepivacaine; however, this was descriptive because the treatment-by-time interaction was not significant. At 24 h, total protein was 2.16 g/dL after ropivacaine and 3.42 g/dL after mepivacaine (P < 0.001). Lactate dehydrogenase activity was lower after ropivacaine than mepivacaine (513 vs. 991 U/L; P = 0.012).ConclusionUnder the conditions studied, ropivacaine produced a clinical response comparable to mepivacaine while being associated with smaller acute changes in the evaluated synovial fluid biomarkers.

The Criollo horse is prized for its endurance, yet its specific biomechanical signatures remain under-researched. This study investigated diagonal dissociation and support patterns in eleven clinically sound Criollo horses to understand the influence of head and neck position (HNP), surface compliance, and morphometry. Using high-speed video (120 fps) and kinematic analysis, we found that diagonal dissociation occurred in 85.24% of hoof contacts, while HNP1 showed a higher frequency of positive (hindlimb-first) dissociation and HNP2 had a higher incidence of negative (forelimb-first) dissociation (p = 0.0398). On soft ground, both HNPs predominantly exhibited hindlimb-first dissociation (p = 0.0446). Soft ground is also associated with the appearance of tripedal support, whereas hard ground presented more synchronous (non-dissociated) diagonal limb support. Thoracic monopedal and tripedal supports are weakly correlated with forelimb measurements, whereas pelvic monopedal and tripedal supports correlate with global body dimensions. All associations were weak to moderate (R² ≤ 0.40). The prevalence of dissociated phases and the absence of a standard symmetrical trot suggest a distinct breed signature adapted for functional efficiency and stability required for its traditional working roles.