Carbon dots (CDs), discovered in 2004, are an emerging member of the carbon nanomaterial family. With their promising properties, CDs have found numerous applications across a wide range of fields. In recent years, their use in agriculture has attracted increasing attention; however, studies on their effects on plant yield and quality remain limited. To address this gap, a field experiment was conducted during the winter season (September–November 2024) at an organic farm in Huong An commune, Hue City, Vietnam (N16°28′21.81″; E107°31′36.996″). The experiment evaluated the effects of CDs applied at seven concentrations (10, 20, 30, 40, 50 and 60 ppm) on lettuce (Lactuca sativa L.) growth, yield traits, and soil physicochemical properties. The findings demonstrated that CD application had a positive effect on lettuce growth and yield, with the highest yield (23.73 t/ha) observed at 60 ppm. Furthermore, CD application at 60 ppm increased soil organic matter by 0.13% and improved both the physical and chemical properties of the soil, resulting in increases in pH (KCl), total nitrogen, total phosphorus and total potassium by 0.45, 0.055, 0.026 and 0.033%, respectively, compared with the treatment without CD application. These results suggest that the foliar application of carbon dots can provide multiple benefits for lettuce production while simultaneously improving soil quality.