Bu etnobotanik çalışma Savaştepe ilçesinde (Balıkesir), Mayıs 2012-Ekim 2015 tarihleri arasında gerçekleştirildi. Savaştepe’de bulunan yerleşim yerleri (44 köy) arazi çalışmaları sırasında ziyaret edildi. Bu çalışmayla, Savaştepe’de (Balıkesir) besin olarak kullanılan bitkilerin geleneksel kullanımlarının belgelenmesi hedeflendi. Yöre halkı ile yüzyüze görüşmeler yapıldı. Bölgede toplam 205 kişi (107 erkek, 98 kadın) ile görüşüldü. Bitki örnekleri yerel halk ile birlikte toplandı. Toplanan türler tayin edildi ve ISTE’de (İstanbul Üniversitesi Eczacılık Fakültesi Herbaryumu) herbaryum örnekleri haline getirilerek saklandı. Toplanan bitkiler arasında 27 familyaya ait 71 taksonun besin amaçlı tüketildiği kaydedilmiştir. Bitkiler çoğunlukla gıda (55 takson), baharat (14 takson), içecek (3 takson) ve ballı macun (2 takson) şeklinde kullanılmaktadır. Çoğunlukla kullanılan bitki bölümleri yaprak (27 takson), toprak üstü kısım (20 takson), meyve (19 takson) ve tohum (5 takson) olarak tespit edildi.

Background/Objectives: Takayasu arteritis is an important non-atherosclerotic cause of ischemic stroke in young adults. However, the relative contribution of systemic inflammation, inherited thrombophilia, and supra-aortic hemodynamic impairment to cerebrovascular events in these patients remains insufficiently defined. This study aimed to evaluate the relative impact of systemic inflammatory activity, hereditary and acquired thrombophilia markers, and supra-aortic vascular involvement on cerebrovascular ischemic events in patients with digital subtraction angiography (DSA) confirmed supra-aortic Takayasu arteritis. Methods: A retrospective cross-sectional analysis was conducted in consecutively evaluated patients with non-atherosclerotic inflammatory stenosis or occlusion of the carotid, subclavian, or vertebral arteries confirmed by digital subtraction angiography. Age- and sex-matched hospital-based individuals without autoimmune, thrombotic, or cerebrovascular diseases served as controls. Laboratory assessments including erythrocyte sedimentation rate, lipoprotein(a), homocysteine, antinuclear antibody, rheumatoid factor, antiphospholipid antibodies, and a hereditary thrombophilia panel were obtained 4–6 weeks after clinical presentation during a stable clinical phase. Results: Among 46 patients with Takayasu arteritis, 21 patients presented with ischemic stroke. The stroke-positive subgroup demonstrated higher inflammatory activity and a slightly greater prevalence of supra-aortic occlusive lesions, particularly involving the common carotid, internal carotid, and subclavian arteries. Although lipoprotein(a) levels showed statistical differences between groups, mean values remained within reference ranges and were not clinically elevated. The distribution of hereditary thrombophilia variants and the prevalence of elevated homocysteine levels did not differ significantly between groups. Clinical outcomes were favorable overall, with no mortality and functional independence achieved in the majority of stroke-positive patients. Conclusions: These findings suggest that systemic inflammation and supra-aortic hemodynamic impairment may play a more prominent role than inherited thrombophilia in the development of cerebrovascular ischemic events in patients with Takayasu arteritis. Selective rather than routine thrombophilia testing may therefore be appropriate in selected clinical contexts, while careful control of inflammatory activity and continuous vascular monitoring remain essential components of management.

In this study, we aimed to reveal the sequence analysis of 69 pharmacogenes in 635 patients and the clinical explanation of variants. Genomic DNA was isolated from peripheral blood of patients. Next-Generation Sequence analysis and bioinformatic analysis were performed to identify 69 pharmacogene variants. Variants with clinical annotation were identified. Analysis of 69 pharmacogenes in a total of 635 patients identified 9485 variants. The number of distinct variants identified in each gene was 1409. Of these 1409 variants, the number of variants registered in PharmGKB was 126. Among the 126 variants registered in PharmGKB, 26 variants were identified that had a direct association with clinical annotation and drug efficacy or toxicity. The most common variant genes were DPYD, CYP2C19, VKORC1,UGT1A1, RYR1 and MTHFR. These 26 variants with clinical annotation were identified in 327 (51%) different individuals. Such a high frequency of critical variants (51%) points to the need for pharmacogenetic studies. The results are extremely important in terms of determining the drug dose according to the genomic status of individuals receiving drug therapy and preventing unnecessary health expenditures.

Aims: The aim of this study was to investigate the clinical significance of mutations in AGO2, DICER and DROSHA genes, which are involved in miRNA biogenesis, as well as TP53, KRAS, BRAF, PI3KCA and APC genes, which are important in the pathophysiology of CRC, and their association with metastasis in patients diagnosed with sporadic colorectal cancer Methods: DNA isolation was performed by taking 10-micron sections from paraffin-embedded tissue samples of 12 patients diagnosed with CRC and Kapa NGS DNA extraction kit was used for sequence analysis. The purity and concentration of the DNA obtained was measured by Qubit fluoremeter, and NadPrep DNA Universal Library Preparation Kit was used for high quality library preparation. Bioinformatics analyses were performed on the Genomize Seq platform. Results: In our study, metastasis was detected in 42% of 12 colorectal cancer patients. Mutations in at least two miRNA biogenesis genes were detected in 80% of metastatic patients. In addition, variants detected in miRNA biogenesis regulatory genes and oncogenic genes were summarized according to pathogenicity status according to the American College of Medical Genetics and Genomics (ACMG) classification. Conclusion: Genes involved in miRNA biogenesis and mutations of clinically relevant genes in CRC have important implications on disease prognosis and response to therapy. Mutations in these genes may be associated with the development of metastases and mechanisms of resistance to treatment and may be potential genetic markers for the development of personalized treatment strategies.

Objectives: This study aimed to investigate the association between two single-nucleotide polymorphisms, rs230416 and rs230429, in the GABBR2 (gamma-aminobutyric acid type B receptor subunit 2) gene and susceptibility to migraine with aura in a Turkish cohort. Patients and methods: In this case-control study, a total of 100 patients (15 males, 85 females; mean age: 33.68 ± 12.16 years; range, 18 to 55 years) with migraine diagnosed according to the International Classification of Headache Disorders criteria and 100 age- and sex-matched healthy controls (25 males, 75 females; mean age: 33.68 ± 12.16 years; range, 18 to 55 years) were genotyped using the polymerase chain reaction-restriction fragment length polymorphism method between January 2018 and June 2013. Genotype and allele frequencies were compared between groups. The Hardy–Weinberg equilibrium, the chi-square test, and Fisher’s exact test were used for statistical analysis ( p < 0.05). Power analysis and genetic equilibrium testing were also performed. Results: For rs230416, genotype distributions were 10% GG, 30% AG, and 60% AA in migraineurs, and 5% GG, 25% AG, and 70% AA in controls ( p = 0.15). Minor allele (G) frequencies were 25% in cases and 17.5% in controls ( p = 0.07). For rs230429, distributions were 10% TT, 38% CT, and 52% CC in patients, and 8% TT, 34% CT, and 58% CC in controls ( p = 0.42), with T allele frequencies of 29% and 25%, respectively ( p = 0.45). All genotype distributions conformed to the Hardy-Weinberg equilibrium. Conclusion: Although this study did not find a significant association between rs230416 or rs230429 and migraine susceptibility, the findings contribute to the growing literature supporting a minor but biologically relevant role of gamma-aminobutyric acidmediated pathways in migraine. Future studies with larger, ethnically diverse cohorts and integrative omics approaches are warranted to clarify the functional impact of GABBR2 variants.

Aim: The objective of this study is to ascertain the association between two prevalent GABRA1 polymorphisms (rs12658835, rs35166395) and migraine with aura . In addition, the investigation will encompass the clinical characteristics of the condition, including trigger patterns and family history. Material and Method: 108 persons with clinically proven migraine with aura and 107 demographically matched healthy controls were enrolled in a multicenter case-control study (Atatürk University Faculty of Medicine Neurology Department and Palandöken State Hospital Neurology Outpatient Clinic; 01 Feb–01 Jun 2013). Sociodemographics, migraine characteristics, triggers, and family history were recorded through structured in-person interviews. A sample of peripheral blood was used to extract genomic DNA. SNP genotyping was performed using a validated real-time PCR allelic discrimination kit for GABRA1 rs12658835 and rs35166395. The case/control status was hidden from the lab staff. Results: The migraine with aura cohort was predominantly female (82.4%) with a mean age of 33.8 ± 12.4 years; the control group exhibited a similar distribution of sex and age (both p > 0.05, Mann–Whitney U for age). rs12658835 exhibited an excess of mutant genotype in migraine with aura (7.4%, 8/108) in comparison to the control group (0%, 0/107), p = 0.007. The genotype distributions of rs35166395 (wild/mutant/heterozygous: 60/16/24% in migraine with aura vs. 65/13/22% in controls) did not differ (p > 0.05). In the context of migraine with aura cases, a family history of migraine was associated with an elevated probability of activity-impairing attacks (69.4% overall; OR ≈ 2.06; p = 0.02) and pain exacerbation during physical activity (p = 0.02). No substantial gender-based disparities were observed in other clinical characteristics. Conclusion: In this Turkish cohort, the GABRA1 rs12658835 variant is associated with migraine with aura, whereas the rs35166395 variant is not. Familial predisposition is linked to more debilitating attacks and exertional exacerbation, highlighting the genetic factors that contribute to the severity of the phenotype and the potential GABAergic mechanisms involved in the pathophysiology of migraine with aura.