Reproductive barriers severely limit interspecific hybridization success among Hydrangea macrophylla, H. paniculata, and H. arborescens, thereby restricting the combination of ornamental traits and cold hardiness. We evaluated cross-compatibility, pollen tube growth, and embryo development in both direct and reciprocal crosses involving H. macrophylla with H. paniculata and H. arborescens. Both species pairs exhibited pronounced unilateral incompatibilities. When H. macrophylla served as the maternal parent, the percentages of seedling emergence were higher, whereas reciprocal crosses produced >84% ovary swelling but resulted in almost no seedlings. Fluorescence microscopy revealed mild prezygotic barriers in direct crosses but strong inhibition of pollen germination and pollen tube growth in reciprocal crosses. Paraffin section observations showed that postzygotic barriers were the primary cause of hybrid failure, with endosperm-type abortion predominating in direct crosses and embryo-type or complete abortion in reciprocal crosses. Consistent with these abortion patterns, direct crosses maintained higher proportions of normal embryos, whereas reciprocal crosses dropped below 10% at the globular stage and approached 0% at later stages. These findings support the use of timely embryo rescue for direct crosses and targeted mitigation of prezygotic barriers in reciprocal crosses to improve Hydrangea interspecific hybridization efficiency.