Puberty health and sex education (PHSE) curricula are not comprehensive or inclusive towards gender-independent, intersex, non-binary, or transgender (GIaNT) youth. A majority of this population seeks this information in online settings. Over the past two years, our research team, in collaboration with GIaNT communities, has developed an open-access online educational resource (OER) intending to bridge this gap in comprehensive PHSE for GIaNT youth in a gender-affirming, fun, and interactive way. After the OER was developed, our team recruited participants (aged 16–26) to navigate our website and complete a multi-method survey to gain their feedback, insights, and perceptions on this new OER. The survey responses indicated two key themes for the development and use of the OER: facilitators and strengths, and areas for improvement. The findings from this study were used to better meet the wants and needs of this population vis-à-vis changes to be made to our OER before its public launch for open-access, free use.