Non-specific Lipid Transfer Proteins (nsLTPs) constitute a ubiquitous family of plant proteins that play a critical role in mediating plant adaptation and tolerance to abiotic stress. While their functions have been extensively characterized in model plants such as Arabidopsis thaliana and rice (Oryza sativa L.), they remain largely unexplored in Cucurbitaceae crops. We identified 31 CmnsLTP genes in the melon (Cucumis melo L.) genome, these genes were unevenly distributed across 11 chromosomes and classified into 8 subfamilies. Members of the same subfamily have similar gene structures and conserved domains, with all family members having motif 1 and motif 3. The promoter region contains cis elements that respond to light, hormones (ABA and MeJA response elements), and abiotic stress, suggesting that this gene is involved in melon growth, development, and stress response. Previous studies have identified copper resistant candidate MELO3C031073.2 through forward genetics, which belongs to the nsLTP family and was named CmnsLTPY.9 in this study. The RT qPCR results showed that the CmnsLTPY.9 exhibited specific expression in different tissues, The expression levels of CmnsLTPY.9 in leaves ranged from 0.3 to 3.2. Under copper stress, the ‘M625’ (copper-sensitive) showed a 3.2-fold increase, indicating marked upregulation. Additionally, CmnsLTPY.9 was localized to the endoplasmic reticulum, and the position remains unchanged after copper stress. This study provides the first systematic analysis of the CmnsLTP gene family in melon; these findings provide fundamental insights into their specific functions in plant development and stress response, as well as valuable genetic resources for future research on copper-tolerant molecular breeding.