In this study, 32 Salmonella strains isolated from 400 chicken carcasses were serotyped, and antibiotic resistance profiles were detected against 12 selected antimicrobial agents using disc diffusion method. Thirty-two isolates were identified as follows; 22 (68.7%) Salmonella Enteritidis, five (15.6%) Salmonella Virchow, three (9.3%) Salmonella Typhimurium and two (6.2%) Salmonella Hadar. In all Salmonella isolates, antibiotic resistance were detected. Out of 32 Salmonella strains, 22 (68.75%) displayed multi-drug resistance. Thirty-two (100.0%) of the isolates were found to be resistant to penicillin G, 20 (62.5%) to nalidixic acid, four (12.5%) to cephalothin, two (6.2%) to streptomycin and two (6.2%) to tetracycline. Fifteen (68.1%) Salmonella Enteritidis, one (33.3%) Salmonella Typhimurium, two (100.0%) Salmonella Hadar and two (40.0%) Salmonella Virchow were shown to be resistant to nalidixic acid. Cephalothin resistance was detected in 9.0%, 33.3%, and 20.0% for Salmonella Enteritidis, Salmonella Typhimurium and Salmonella Virchow, respectively. The results indicate that Salmonella recovered from chicken carcasses were resistant to multiple antimicrobials and that resistance among these isolates varies by serotype. Also, this emerged as a significant public health problem.
Swab samples from cattle and sheep carcasses (120 of each) were tested for Listeria monocytogenes, and 120 slaughterhouse wastewater samples were tested for listeriophages over 12 months (10 samples per month) to note the seasonal distribution. L. monocytogenes and bacteriophage isolates were characterized, and the biocontrol of L. monocytogenes was investigated in meatballs with a phage cocktail. L. monocytogenes was found in 3.4 and 2.5% of cattle and sheep carcasses, respectively. All the isolates were found to harbor hlyA, actA, inlA, inlB, inlC, inlJ, plcA, plcB, fbpA, and fri genes with varied mRNA expression levels by real-time reverse transcriptase PCR analysis. Five isolates did not harbor the vip gene. According to enterobacterial repetitive intergenic consensus PCR, L. monocytogenes isolates were classified into four different groups based on their DNA patterns. The L. monocytogenes isolates were characterized for antibiotic susceptibility; one strain was found to be resistant to five ...