Paleoecological studies of continental gastropods give valuable information about the depositional environment of the fossil assemblages. In South America, these assemblages from the Late Cretaceous/Paleocene are scarce and poorly studied. Most works focuses on taxonomy, but a paleoecological approach is still missing. We analyzed the assemblages present in the Queguay limestones from Uruguay. The total fossil content consists in freshwater and terrestrial gastropods, characean gyrogonites, ostracods, Neosauropoda eggshells, vegetable remains, insect nests, and pupal chambers. As the precise age and sedimentary environment of the Queguay limestones have been discussed for almost nine decades, a paleoecological study was conducted in order to answer these questions from this point of view. Diversity (Simpson, Shannon-Wiener, equitability) and similarity (Jaccard and Kulczynski) indices were calculated; the analyses showed a close relationship among all locations, and therefore we conclude that there are not differences in fossil content among the localities. Moreover, the data allows to consider them basically of the same environment and age. The presence of Neosauropoda eggshells in several outcrops indicate unquestionably a Late Cretaceous age, and establish the oldest record worldwide for Pupillidae, Orthalicoidea and Biomphalaria.