In this study, we examined the psychometric properties (reliability, validity, and underlying factor structure) and measurement invariance of a researcher-developed instrument: Motivated Strategies for Science Learning (MSSL). Data were collected from 2765 fifth-grade students across [state withheld] school districts. Results from exploratory and confirmatory factor analyses suggested satisfactory psychometric properties of MSSL. We further identified strong evidence to support configural, metric, scalar, and strict invariance across school location groups (i.e., rural and non-rural), confirming that the instrument can accurately capture the construct of science learning motivation among fifth-grade students from both rural and non-rural Texas schools. The study provides the first step toward measuring Texas rural and non-rural school students’ motivation and strategies for science learning. Recommendations for future research are discussed.