Oral biofilms are complex polymicrobial communities involved in the development of dental caries and periodontal diseases. Chemical antiseptics are commonly used as adjuncts to mechanical plaque control; however, their antimicrobial efficacy varies depending on composition and mechanism of action. The aim of this study was to comparatively evaluate the antimicrobial and antibiofilm activities of four commercially available dental products (Corsodyl, Ozosan, HybenX, and Elugel) against a broad spectrum of oral microorganisms. This in vitro study included Gram-positive and Gram-negative bacterial strains, comprising both reference strains and clinical isolates, as well as Candida albicans. Antimicrobial activity was assessed using the disc diffusion assay, while antibiofilm activity was evaluated using a crystal violet microplate assay. All experiments were performed in triplicate. Statistical analysis was conducted using two-way ANOVA followed by Tukey’s post hoc test (p < 0.05). All tested products exhibited antimicrobial activity. Inhibition zones ranged from 9 to 56 mm for Gram-positive bacteria, 12 to 38 mm for Gram-negative bacteria, and 13 to 43 mm for Candida albicans. Two-way ANOVA revealed a significant effect of the dental product (p < 0.001), while incubation time was not significant (p > 0.05). HybenX showed the highest antimicrobial efficacy, while chlorhexidine-based products demonstrated consistent activity. Antibiofilm inhibition exceeded 80% for several strains. Dental antiseptics exhibit significantly different antimicrobial and antibiofilm profiles, highlighting the importance of appropriate product selection in oral biofilm control.

This research investigate the impact of sugar consumtion on salivary pH,highlighting its implication for oral health.1.Background/Objectives: The study aimed to analyzes the effect of excessive sugar intake,a key factor in the development of dental condition.2.Methods: The study involved 45 students aged 20-23 years.Saliva samples were collected at four intervals:before sugar intake,immediately after,30 minutes later,and one hour post-consumtion.Samples were obtain via drainage,and pH was measuredusing indicator strips.3.Results:The data revealed a significant drop in salivary pH immediately after sugar consumtion,decreasing from an initial pH of 7 to 5.5.Partial recovery was observed,with pH reaching 6.5 after one hour.This temporary acidification of the oral enviroment confirms the link between sugar intake and an increased risk of dental caries.4.Conclusion: The study emphasizes the importance of maintaining optimal salivary pH to prevent enamel demineralization and support natural remineralization.It also underscore the need for public education to reduce sugar consumtion and adopt effective oral hygiene practices.This research contrubutes to undestanding the dietary impact on oral health and suggests preventive measures to lower the incidence of dental issues.

Aim of Study: This study aimed to assess the effectiveness of two disinfection methods—chemical-based cleaning with antibacterial tablets and a combination of UVC light and tablets—on silicone interception devices used in interceptive orthodontic treatment. Materials and Methods: Four devices were sectioned into three equal parts and divided into three groups: control (untreated), chemically cleaned (MyOClean antibacterial tablets), and disinfected with UVC light and tablets. Microbial samples were collected, cultured on UTI Chromogenic and Blood Agar media, and analyzed for bacterial growth and antibiotic susceptibility. Results and Discussion: The control group showed bacterial positivity within 24 hours, whereas the chemically and UVC-treated groups delayed bacterial growth to 48 hours, demonstrating the effectiveness of these methods in reducing bacterial contamination. Antibiograms of Staphylococcus aureus revealed high susceptibility to most antibiotics, with intermediate susceptibility noted for ciprofloxacin. Conclusion: The combination of UVC light and antibacterial tablets showed statistically superior disinfection outcomes (p ≤ 0.05) and represents a promising strategy for improving the hygiene of orthodontic appliances. These findings provide evidence-based recommendations for reducing microbial contamination and addressing antimicrobial resistance in orthodontic practice.

Saliva, a non-invasive and accessible biological fluid, has emerged as a valuable medium for monitoring health and disease. This study explores the impact of smoking on the total antioxidant capacity of saliva (TAC), a critical defense against oxidative stress. Conducted as a cross-sectional study from December 2022 to February 2023, the research involved students from the Faculty of Dental Medicine in Timișoara. Unstimulated saliva samples were collected using standardized procedures to ensure reliability and analyzed with a Trolox-based antioxidant test kit (ab65329, Abcam). Among the 70 participants, 40% identified as smokers, with varying cigarette consumption patterns. Results demonstrated that smoking significantly reduced salivary antioxidant levels, indicating a depletion of natural defenses against free radicals. Smokers exhibited a lower TAC compared to non-smokers, supporting a strong causal relationship between smoking and oxidative stress. These findings highlight smoking’s detrimental effects on systemic antioxidant status and oral health, underscoring the necessity for targeted preventive strategies and interventions to mitigate associated health risks. This study contributes to the growing evidence that smoking compromises biological defense systems, with implications for broader health outcomes and public health policy.

Background/Objectives: Glucagon-like peptide-1 receptor agonists (GLP-1RAs) are widely used to treat type 2 diabetes and obesity, yet their periodontal, peri-implant, salivary, and oral microbial effects remain incompletely characterized. This scoping review mapped the clinical and preclinical evidence on GLP-1RA-related oral effects, investigated mechanisms, and knowledge gaps. Methods: PubMed, Scopus, and the Web of Science Core Collection were searched from inception to 9 September 2026. Original human, animal in vivo, in vitro/ex vivo, and mixed-design studies were eligible when a GLP-1RA was a principal intervention or exposure and a relevant oral or orofacial outcome was reported. Two reviewers independently screened records. Study characteristics, interventions, outcomes, and findings were charted and synthesized descriptively. The review followed Joanna Briggs Institute methodology and PRISMA-ScR guidance and was registered on the Open Science Framework after study selection but before completion of the methodological appraisal and manuscript. Results: Twenty-seven publications were included, 23 of which were wholly or partly preclinical. Four publications were exclusively human, and one mixed-design publication included an additional human observational component. Experimental findings suggested anti-inflammatory, antioxidant, and osteogenic effects, but molecular responses were not consistently accompanied by structural benefits. Evidence concerning salivary function, oral microbial changes, and orofacial adverse events was limited and heterogeneous. Human findings were derived from non-randomized observational and descriptive designs. Conclusions: Evidence of potential oral effects is predominantly experimental, and human data are insufficient to establish clinical benefit or causality. Prospective controlled studies with standardized clinical outcomes are needed to determine whether experimental findings translate into meaningful oral-health benefits.