Macadamia is a kind of evergreen nut trees which belong to the Proteaceae family. The two commercial macadamia species, Macadamia integrifolia and M. tetraphylla , are highly prized for their edible kernels. Catherine et al. reported M. integrifolia genome using NGS sequencing technology. However, the lack of a high-quality assembly for M. tetraphylla hinders the progress in biological research and breeding program. In this study, we report a high-quality genome sequence of M. tetraphylla using the Oxford Nanopore Technologies (ONT) technology. We generated an assembly of 750.54 Mb with a contig N50 length of 1.18 Mb, which is close to the size estimated by flow cytometry and k-mer analysis. Repetitive sequence represent 58.57% of the genome sequence, which is strikingly higher compared with M. integrifolia . A total of 31,571 protein-coding genes were annotated with an average length of 6,055 bp, of which 92.59% were functionally annotated. The genome sequence of M. tetraphylla will provide novel insights into the breeding of novel strains and genetic improvement of agronomic traits.

Elaeagnus conferta Roxb is a kind of fruit known for its oval shape, bright red colour, and sour and sweet taste. In this study, we assembled and characterized the complete chloroplast genome of E. conferta Roxb as a resource for future genetic studies. With a total length of 151,751 bp, the chloroplast genome has a total of 130 genes, consisting of 88 protein-coding genes, 34 tRNA genes, and 8 rRNA genes. Phylogenetic analysis confirmed the position of E. conferta Roxb within the order Rosales.

The complete chloroplast genome sequences of Manilkara zapota (Linn.) van Royen in Xishuangbanna, Yunnan province were reported in this study. The length of the sequence was 159,853 bp long with the large single copy (LSC) region of 89,632 bp, the small single copy (SSC) region of 18,747 bp, and two inverted repeat (IR) regions of 27,737 bp. The plastome contained 125 genes, including 84 protein-coding, 8 ribosomal RNA, and 33 transfer RNA genes. The overall GC content was 37.0%. Phylogenetic analysis of 12 representative plastomes within the order Ebenales suggests that M. zapota (Linn.) van Royen is closely related to the species in family Ebenaceae.

Moringa oleifera is a small to medium-sized, evergreen or deciduous tree that native to northern India, Pakistan and Nepal. In this study, we report the complete chloroplast genome sequence of M. oleifera for the first time (accession number of MH778650), and it is also the first reported chloroplast genome of Moringaceae. The chloroplast genome is 160,599 bp long and includes 113 genes. Its LSC, SSC and IR regions are 88,933, 19,482 and 26,092 bp long, respectively. Phylogenetic tree analysis exhibited that M. oleifera was clustered with other Moringaceae species with 100% bootstrap values.

The rubber tree (Hevea brasiliensis) is increasingly affected by climate change, particularly by intensified water-deficit stress, which impacts latex yield and plantation productivity. Understanding how rubber trees adapt to water-deficit stress at the molecular level is crucial for improving their resilience. In this study, we generate a high-resolution single-cell transcriptomic atlas to investigate the cellular and molecular mechanisms underlying the response of rubber tree bark to water-deficit stress. Using droplet-based single-cell RNA sequencing, we profile 17,994 individual cells isolated from control, 4-day, and 7-day drought-stressed bark tissues. We characterize seven major cell types with 12 transcriptionally distinct cell clusters. Differential gene expression analysis reveals significant transcriptional changes, with a notable upregulation of genes involved in abscisic acid (ABA) signaling, metabolic adaptation, and stress response pathways. Additionally, we find that the ABF transcription factors (ABF2, ABF3, and ABF4) are consistently upregulated across all major cell types in response to water-deficit stress. These findings offer valuable insights into the cellular and molecular mechanisms that enable rubber trees to adapt to drought, providing a foundation for future efforts aimed at enhancing drought tolerance in this important economic species.

The chloroplast (cp) genome sequence of Persea americana Mill. has been characterized from Illumina pair-end sequencing. The complete cp genome was 152,732 bp in length, containing a large single copy region (LSC) of 84,632 bp and a small single copy region (SSC) of 18,346 bp, which were separated by a pair of 24,877 bp inverted repeat regions (IRs). The genome contained 126 genes, including 86 protein coding genes, 32 tRNA genes, and eight rRNA genes. Further, phylogenetic analysis of 10 representative sequences within the order Ebenales suggests that Persea americana Mill. is closely related to the species in family Sapotaceae.