Amaç: Bruselloz; dünyada yaygın bakteriyel zoonoz olup ülkemizde de sık olarak görülebilmektedir. İnsanlarda gelişen bruselloz hastalığının tanısında başlıca kültür ve serolojik yöntemler kullanılmaktadır. Biz hastanemizde bruselloz serosürveyansını görmeyi amaçladık. Yöntem: Temmuz 2014-Temmuz 2019 tarihleri arasında hastanemiz mikrobiyoloji laboratuvarına gönderilen bruselloz açısından şüpheli örnekler Rose Bengal ve testi ile değerlendirilmiş olup Rose Bengal testinde aglütinasyon gözlenen örnekler pozitif sayılmıştır. Brucella Coombs jel testinde ise önce hasta örneklerinin seumları, hastalar için ayrılan kuyular içinde yapılmış olup (1/40-1/5120) 1/160 ve üzerindeki titreler pozitif olarak kabul edilmiştir. Bulgular: Çalışmaya dahil edilen 13796 bruselloz şüpheli hasta örneğinden Rose Bengal testi pozitif 1040 (%7,5) hasta bulunmuştur. Brucella Coombs jel testi pozitif olan hasta sayısı ise 468 (%3,4) dir. Rose Bengal testi pozitif olup Brucella Coombs jel testi negatif olan 615 (%4,5), Rose Bengal testi negatif olup Brucella Coombs jel testi pozitif olan 43 hasta (%0,3) bulunmuştur. Sonuç: Rose Bengal ve Brucella Coombs jel testi kullanılarak hastanemizde son 5 yıldaki brusella serosürveyansını araştırdık. Son yıllarda kullanımı yaygınlaşan Brucella Coombs jel testi ile elde ettiğimiz sonuçların birçok laboratuvarda kullanılan Standart tüp aglütinasyon testi ile uyumlu olduğunu özellikle blokan antikorlar varlığında sonuçların daha güvenilir olabileceğini ve ilerde birçok merkezde kullanılabileceğini gösterdik.

Aim: Blood culture is the gold standard in the diagnosis of bacteraemia and sepsis. When antimicrobial treatment is delayed, severe infections may occur, which may cause high morbidity and mortality. We aimed to determine the distribution of Enterobacterales isolates and their antimicrobial susceptibility profile from blood culture samples sent to the laboratory between 2016 and 2021. Material and Method: Enterobacterales isolated from blood culture samples sent to Ondokuz Mayis University Medical Microbiology laboratory between January 2016 and December 2021 was enrolled in the study. Vitek MS (BioMérieux, France) was used for identification of bacterial species and Vitek2 (BioMérieux, France) Compakt automated system were used for determination of antibiotic susceptibility of bacteria. EUCAST criteria were used for the evaluation of antibiotic susceptibility of the isolates. Results: The most three isolated bacteria were Escherichia coli, Klebsiella spp. and Enterobacter spp. respectively. The resistance rates were 9.06% for meropenem, 8.89% for imipenem and 15.67% for ertapenem, 7.57% and 6.28% for amikacin and tigecycline, respectively. Conclusion: Since the antibiotic resistance rates of the agents causing bloodstream infections show regional differences, it is thought that screening epidemiological data and reporting the results will increase the correct treatment rate and decrease the mortality rate.

Lower respiratory tract infections are an important cause of morbidity and mortality. Identification of infectious etiology is very important for specific treatment. In addition to routine methods in diagnosis, polymerase chain reaction-based tests have been used. By identifying pathogenic organisms and specific antibiotic resistance markers earlier than routine methods, these tests have the potential to accurately detect agent and reduce the duration of antibiotic therapy. The aim of this study is to show that the use of multiplex polymerase chain reaction-based method may be useful in early diagnosis and specific treatment. In this study, 283 lower respiratory tract samples sent to Ondokuz Mayıs University Faculty of Medicine Hospital Molecular Microbiology Laboratory between 01/08/2021–01/04/2023 were retrospectively analyzed. Lower respiratory tract samples were studied with BioFire® FilmArray Pneumonia Panel plus (BioMérieux, France). The lower respiratory tract samples of the patients sent at the same time were planted on 5% sheep blood agar, chocolate and EMB agar and incubated at 36° for 24 h. Identification of bacteria was performed by Vitek MS. Antibiotic sensitivities were studied in Vitek2 Compact automated systems. Two hundred and eighty-three patient samples were included in the study; 156 (55%) of the samples were bronchoalveolar lavage samples and 127 (45%) are sputum samples. When panel and culture results were compared, compatible results were found in 70%. In 88 (61%) of the patients with no growth in culture, no agent was detected as a result of the panel or only viral agents were detected. MecA/C and MREJ resistance genes were detected in 26% of 46 patients with S. aureus detected in the pneumonia panel. CTX-M resistance gene was detected in a total of 28 samples in the pneumonia panel. The OXA-48 carbapenem resistance gene was detected in 14 samples. In our study, it has been shown that Multiplex-PCR has an important place in detecting atypical bacterial and viral factors that do not grow in routine culture in lower respiratory tract infections. In addition, by identifying the resistance genes in the detected bacterial agents, it will contribute to the correct initiation of empirical treatment and to prevent unnecessary antibiotic use in lower espiratory tract diseases where viral factors play a role.