Per-and polyfluoroalkyl substances (PFAS) have been a global concern in relation to human exposure. Dust has been proven to be an important source of human exposure to many groups of organic pollutants, however, no study so far has systematically evaluated human exposure to PFASdepending on time spent in different indoor environments i.e., houses, offices and public transport vehicles. Archived dust samples were collected from residential houses (n=38), offices (n=15), and public transport vehicles (n=12) in Australia to assess human exposures to PFAS via dust ingestion and dermal contact. Amongst the 19 target analytes, 14 compounds were detected in >50% of the samples. Significantly higher PFAS concentrations were found in dust samples collected from offices with mean±SD of 400±810 ng/g than houses (170±350 ng/g) and public transport vehicles (39±33 ng/g). Composition patterns of PFAS varied among indoor environments with Perfluorohexanoic acid (PFHxA) (median 2.5 ng/g dust) and perfluorooctanoic acid (PFOA) (median 5.7 ng/g) were the most prevalent compounds detected (DF=100%). PFOA was the dominant PFAS compound in both offices (median 11 ng/g) and houses (median 5.8 ng/g). Perfluorododecanoic acid (PFDoA) (median 3.6 ng/g) was found to be the most abundant compounds in public transport vehicles. Considering the time spent in indoor environments, relatively higher estimated daily intakes (EDIs) were identified in offices than in houses and public transport vehicles. EDIs for PFOA and perfluorooctane sulfonic acid (PFOS) for adults and toddlers were well below the acceptable levels of 20 ng/kg/day proposed by U.S Environmental Protection Agency (USEPA) and Food Standard Australia and New Zealand (USEPA, 2016; FSANZ, 2016), suggesting low or negligible exposure pathways via dust ingestion and dermal contact.