With the rising incidence of drug-resistant pathogens, focus should be placed on biologically active compounds derived from plant species used in herbal medicine, as these compounds may provide a new source of antifungal and antibacterial activities. The aim of this study was to evaluate the antifungal and antibacterial activity of ethanol and aqueous extracts from different parts of Rosa rugosa. In order to evaluate the antimicrobial activity of the extracts of R. rugosa rose hips, flowers, petals, leaves, stems, and roots, a laboratory microbiological test was performed using the well diffusion method in agar. A rotary evaporator was used for extract concentration and extractant removal. Antimicrobial activity was tested against one fungus, four Gram-positive, and four Gram-negative bacteria. The leaf extracts exhibited the strongest antimicrobial activity, followed by the extracts from the petals and rose hips, while weaker activity was observed in the root extracts. The extracts from the stems and rose hips showed the weakest effect. Ethanol extracts were more effective than water extracts. Aqueous and ethanolic extracts of R. rugosa parts demonstrated antifungal activity against Candida albicans, with ethanol extracts proving to be more effective. Among all the R. rugosa parts analyzed, the petals exhibited the strongest antifungal activity.

Eye cosmetic products are widely used and applied in close proximity to the ocular surface, making their microbiological safety particularly important. The aim of this study was to assess bacterial contamination in used eye cosmetic products, characterize the antimicrobial resistance profiles of the isolated bacteria, and perform molecular genotypic analysis. A total of 71 samples, including mascara, eyeliner, and eyeshadow, were analyzed. Microbiological analysis revealed that Bacillus spp. and coagulase-negative staphylococci (CoNS) were the predominant microorganisms, while no major pathogens such as Staphylococcus aureus, Escherichia coli, or Pseudomonas aeruginosa were detected. Antimicrobial susceptibility testing demonstrated high susceptibility of isolates to gentamicin, vancomycin, and linezolid, whereas resistance to benzylpenicillin and clindamycin was observed among Staphylococcus spp. Molecular identification based on 16S rRNA gene sequencing confirmed the presence of Bacillus licheniformis, Bacillus subtilis, Staphylococcus epidermidis, and Staphylococcus warneri, with sequences showing high similarity to globally distributed strains. Although the detected microorganisms were predominantly opportunistic, their presence in products applied near the eyes suggests a potential risk of microbial transfer to the ocular surface. These findings highlight the importance of proper hygiene practices, regular product replacement, and effective quality control measures to minimize microbial contamination and associated health risks.