Wild ungulates represent valuable bioindicators of environmental quality due to their exposure to contaminants through diet and habitat. This study assessed concentrations of selected toxic and trace elements and evaluated biochemical parameters in red deer (Cervus elaphus, n = 37) and roe deer (Capreolus capreolus, n = 8) to determine their health status, characterize environmental conditions, and compare interspecific differences. Blood samples were analysed for elements (As, Cd, Cr, Cu, Mn, Hg, Tl, Zn) using ICP-MS and for biochemical parameters (TP, ABL, GLO, ALT, AST, ALP, GLU, CHOL, TG, CRE, BUN, Ca, P). Cadmium and zinc concentrations were significantly higher in roe deer (p = 0.012; p = 0.018), while other elements did not differ significantly (p > 0.05). Manganese showed high variability in both species. No statistically significant interspecific differences were found in biochemical parameters. Slight elevations in ALT, AST, and ALP were likely associated with capture-and handling-related stress rather than pathological conditions. The animals were kept in a game reserve and supplemented with feed during the winter months, which may contribute to a more stable nutritional status and reduced variability in biochemical profiles. Overall, the low levels of toxic elements and absence of major biochemical alterations suggest a relatively unpolluted environment.