Drought is one of the most common abiotic stresses, affecting the growth and productivity of crop plants globally, particularly in arid and semi-arid regions. Different strategies are used to mitigate the impact of drought among crop plants. Exogenous application of different substances are known to decrease the effects of various abiotic stresses, including drought stress. The aim of this study was to evaluate the effect of Ca²⁺ and H₂O₂ in developing drought stress tolerance in Brassica napus “Bulbul-98” seedlings. Brassica napus “Bulbul-98” seedlings were exposed to 5, 10 and 15 mM Ca²⁺ and 2, 5 and 10 μM H₂O₂ concentrations twice at an interval of two days for up to 20 days after germination. Drought stress decreased relative water content (RWC), chlorophyll content and increased proline, H₂O₂, soluble protein and electrolyte leakage in Brassica seedlings. Exogenous Ca²⁺ (5, 10,15 mM) and H₂O₂ (2, 5, 10 μM) supplementations, during drought stress induction, showed a significant increase in RWC by 5.4%, 18.06%, 26.2% and 6.87%, 13.9%, 18.3% respectively. Similarly, with the exogenous application of Ca²⁺ (5, 10, 15 mM) and H₂O₂ (2, 5, 10 μM), chlorophyll content was increased by 15.03%, 22.2%, and 28.4%, and 9.6%, 23.3%, and 27.5% respectively. It was confirmed that the seedlings under drought stress that were supplemented with Ca²⁺ and H₂O₂ recovered from water content reduction and chlorosis, and were able to grow normally.

Globally, drought and salinity stress critically constrain potato (Solanum tuberosum L.) production. Considering the impact of these stresses on crops and increasing food demand, insight into both tolerance and susceptibility is essential. The present study screens two potato cultivars, BARI-401 and Spunta, for their tolerance to simulated salinity and drought by in vitro LiCl and mannitol exposure. Plantlets treated with a range of LiCl (0, 10, 30, and 40 mM) and mannitol (0, 50, 100, 200, and 250 mM) concentrations were biochemically and physiologically characterized to assess their tolerance capacity. Shoot number, shoot length, root number, and root length were affected in both cultivars under higher LiCl and mannitol concentrations, even though Spunta was able to better maintain a higher shoot length under the 40 mM of LiCl and 250 mM of mannitol compared to BARI-401. The total phenol contents (TPC) in both cultivars were increased at the highest treatment concentration and the total flavonoids content (TFC) was decreased in BARI-401 as compared to Spunta. Higher free radical scavenging capacity (FRSC, low IC₅₀ value) was recorded in Spunta as compared to BARI-401 with increasing treatment concentrations, which supports the high antioxidant capacity of Spunta. An inverse correlation between polyphenol oxidase (PPO) and TPC was noted in both cultivars. Peroxidase dismutase (POD) activity was increased significantly in both cultivars for all treatments, but activity was highest overall in Spunta. These physiological and biochemical analyses of both cultivars suggest that cultivar Spunta is more tolerant to salinity and drought stress. Further open-field experiments are required to confirm these results.

The present study assesses the effect of drought stress on the Moringa biotype under magnetized water treatment (MWT). The Moringa biotype seedlings were subjected to drought stress with varying field capacities (FC) viz., control (100% FC), moderate drought stress (MS,50% FC), and severe drought stress (SS, 20% FC). Magnetized water (MW) significantly increased plant height, leaflet number, internode distances, leaf area, dry weight of the leaf, shoot, root of the seedlings and markedly improved the assimilation, transpiration, stomatal conductance, water use efficiency and vapor pressure deficit under drought stress conditions. The maximum quantum efficiency of PSII (Fv/Fm) and maximum chlorophyll fluorescence (Fm) were increased and minimum chlorophyll fluorescence (F₀) in the darkadapted state was decreased under drought stress with MWT. Photosynthetic pigments (Chl a, Chl b, carotenoids) significantly decreased under drought stress, but MW significantly increased them. The MW application in Moringa biotype resulted in a decrease in total phenolic content (TPC) by 19% and 26% under MS and SS conditions, respectively. Malondialdehyde(MDA), hydrogen peroxide(H₂O₂) and accumulation of proline in leaf were decreased with the prolongation of drought with MW. MW could be used for alleviating the drought stress in Moringa biotype seedlings and improve drought tolerance by modulating the physiological activities.

A total of 15 fungal isolates were obtained from oil-contaminated sites near the Red Sea in the Yanbu region. Based on the preliminary DCPIP (2,6-dichlorophenolindophenol) assay, three isolates showed promising oil degrading ability. The next-generation sequencing of the ITS-I and ITS-II internal transcribed spacer regions assigned the isolates to Aspergillus and Penicillium. Among these three strains, Y2 (Aspergillus oryzae) was the most efficient, degrading about 99% of the crude oil. The degradation rates were corroborated using spectrophotometric and gas chromatography–mass spectrometry analyses after two weeks of cultivation in Bushnell–Haas medium. All the three strains proved to be potent oil-degrading strains and, hence, can be utilized to degrade oil contaminants.

MnO₂ and MnO₂ blended with 1 and 2 weight percent of activated carbon (AC), MnO₂/AC1 and MnO₂/AC2 were synthesized through the sol–gel method. The pure chitosan (CS) films were cast in the form of films. Similarly, 5 weight% of each MnO₂, AC, MnO₂/AC1 and MnO₂/AC2 was intermingled with the CS to produce different films, such as CS-AC, CS-MnO₂, CS-MnO₂/AC1 and CS-MnO₂/AC2. Zero-valent Co NPs were then supported on these films through the chemical reduction method and expressed as CS@Co, CS-AC@Co, CS-MnO₂@Co, CS-MnO₂/AC1@Co and CS-MnO₂/AC2@Co NPs. All the catalysts were characterized by field emission scanning electron microscopy (FESEM), energy-dispersive spectroscopy (EDS) and X-ray diffraction (XRD) techniques. The synthesized catalysts were used as a dip catalyst against the hydrogenation of 4-nitrophenol (4NP), and for the degradation of methyl orange (MO) and Congo red (CR) dyes. The k_app and R² values were deduced from pseudo-first-order kinetics for 4NP and MO and zero-order kinetics for CR dye. The k_app values of CS-AC@Co and CS-MnO₂/AC1@Co NPs for 4NP hydrogenation were higher than those for any other member of the series, at 1.14 × 10⁻¹ and 1.56 × 10⁻¹ min⁻¹ respectively. Similarly, the rate of CR degradation was highest with CS-AC@Co. The R² values for 4NP, MO and CR dyes were above 0.9, which indicated that the application of pseudo-first- and zero-order models were appropriate for this study. Furthermore, the antibacterial activity of all the catalysts was evaluated against Pseudomonas aeruginosa and Escherichia coli. The CS-AC@Co NPs exhibited the highest zone of inhibition compared to other catalysts against P. aeruginosa, while all the catalysts were inactive against E. coli. This study reveals that the catalyst can be used for the degradation of other pollutants and for microbial inhibition.

The development of a solid substrate for the support and stabilization of zero-valent metal nanoparticles (NPs) is the heart of the catalyst system. In the current embodiment, we have prepared solid support comprise of alginate-coated cellulose filter paper (Alg/FP) for the synthesis and stabilization of Co nanoparticles (NPs) named as Alg/FP@Co NPs. Furthermore, Alginate polymer was blended with 1 and 2 weight percent of CoNi NPs to make Alg-CoNi1/FP and Alg-CoNi2/FP, respectively. All these stabilizing matrixes were used as dip-catalyst for the degradation of azo dyes and reduction of 4-nitrophenol (4NP). The effect of initial dye concentration, amount of NaBH₄, and catalyst dosage was assessed for the degradation of Congo red (CR) dye by using Alg-CoNi2/FP@Co NPs. Results indicated that the highest k_app value (3.63 × 10⁻¹ min⁻¹) was exhibited by Alg-CoNi2/FP@Co NPs and lowest by Alg/FP@Co NPs against the discoloration of CR dye. Furthermore, it was concluded that Alg-CoNi2/FP@Co NPs exhibited strong catalyst activity against CR, and methyl orange dye (MO) degradation as well as 4NP reduction. Antibacterial activity of the prepared composites was also investigated and the highest l activity was shown by Alg-CoNi2/FP@Co NPs, which inhibit 2.5 cm zone of bacteria compared to other catalysts.