Arbuscular mycorrhizal fungi (AMF) play an important role in grapevine production systems. However, little is known about how this relationship is achieved in the nursery and how soil management might modify it and its derived benefits. Here, we review the current knowledge on the establishment of grapevine–AMF relationships from the nursery to the field, the main factors that affect the effectiveness of the symbiosis, the potential role of AMF as biostimulants in grapevine production systems, and the future perspectives of their use in the current context of climate change. The process of establishing mycorrhizal symbiosis is complex, and the molecular dialogue between the plant roots and the fungus is still not yet fully understood. During vine plant production, rooting occurs in nurseries, where spontaneous symbiosis can be generated. The effectiveness of mycorrhizal symbiosis appears to depend not only on the identity of the fungus but also on the diversity of the vine material and soil management. Finally, the use of AMF as biostimulants might be an effective strategy with which to face the new climatic scenario, but further research dealing with the application of AMF inocula and the protection of native cohorts should be conducted.

Grafting has been fundamental in viticulture since the phylloxera crisis of the late 19th century; nevertheless, the functional consequences of vascular connection on the graft union remain poorly understood. The effects of grafting on Vitis vinifera cv. Tempranillo (Te) were evaluated using two complementary approaches: (1) cambial alignment, comparing completely aligned (CA) versus partially aligned (PA) unions; and (2) grafting and scion–rootstock interaction, comparing heterografts (Te/110R and Te/RG8), homografts (Te/Te), and ungrafted Te cuttings. These approaches were tested through three experiments: a vineyard trial and two pot trials under well‐watered (WW), moderate water stress (MWS), and recovery (R) regimes. In the vineyard, CA plants exhibited greater vegetative growth and gas exchange, particularly on 110R, whereas the vigorous RG8 rootstock mitigated the effects of misalignment. Under MWS conditions, CA adopted a drought‐avoidant strategy with earlier stomatal closure and higher root allocation, whereas PA maintained higher stomatal conductance, recovered photosynthesis faster after rewatering, and prioritised shoot and rootstock growth, especially on RG8. Finally, grafted plants were more sensitive to water stress than ungrafted plants, while homografts accumulated the greatest biomass and root investment, suggesting more efficient vascular connectivity compared with heterografts. Our study highlights that cambial alignment, grafting, and partner interactions influence plant development and physiological performance; however, long‐term studies are needed to clarify how vascular connectivity at the graft union affects transport processes, stress responses, and ultimately vine longevity under different scion–rootstock combinations.

Rootstock mother fields supply the cuttings used in the grafted grapevine propagation process, and their productivity is essential for the nursery production. In southern Europe, mother fields are usually managed with a ground-level pruning system, which provides high yields but increases sanitary and management risks. Dense canopies favour humid microclimates and large pruning wounds increase the incidence of trunk diseases. Trellis systems have been proposed as alternatives to mitigate these risks, but their productive performance remains unclear. The aim of this study was to evaluate the vegetative growth of three vertical trellis systems: elevated crown, alternate crown, and vertical axis, in two rootstock mother fields, and to compare their cutting productivity with that of the traditional ground-level system once the trellis vines were fully established. The trial was conducted from 2022 to 2024 in a commercial rootstock mother field in Soria, Spain, using two Vitis berlandieri × V. rupestris hybrids (110 Richter and 140 Ruggeri). The experimental design consisted of three trellis systems arranged in four replicates of 12 vines each, following a randomised block design. Pruning weight increased significantly with vine age but was unaffected by trellis system. Cutting yield differed between rootstocks, with 140 Ruggeri producing about twice as many cuttings as 110 Richter. The comparison between trellis and ground-level systems showed that the former increased labour requirements and reduced cutting yields. Although trellis systems challenge their adoption for commercial rootstock propagation under the dry-summer conditions of this study, their potential to reduce fungal disease incidence and improve canopy management may offer advantages in a transition towards a more sustainable nursery process.

This study addresses the early detection of water stress in grapevines (Vitis vinifera L. cv. Monastrell), a key challenge for precision irrigation. The main objective is to assess the feasibility of VIS–NIR hyperspectral imaging (400–1000 nm) to anticipate water stress, relating the spectral signal to stem water potential. This study was developed over two campaigns, in 2024 and 2025, using 18 potted plants. In 2024, eight vines were irrigated, and the remaining 10 were subjected to water-deprivation treatments, whilst in 2025, all plants were irrigated, but half at a control dose and the rest at a reduced dose equivalent to 33% of the control. Images were acquired over five dates in June 2024 and over seven in June 2025 using a Specim IQ camera; stem potential was also measured to provide a physiological reference. Individual time series were developed, calculating the Mahalanoubis distance in a PCA space. Results revealed a change window between 10 and 13 June, consistent with the divergence in water potential from 17 to 24 June. PCA highlighted spectral regions related to changes in pigments, nitrogen and water content as main indicators of water stress. We conclude that HSI is a promising tool for early water stress detection.