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Maternal exposure to environmental pollutants affects fetal development, which can result in hypertension in adulthood. Gut microbiota-derived metabolite trimethylamine (TMA), trimethylamine-N-oxide (TMAO), and short chain fatty acids (SCFAs) have been associated with hypertension. We tested a hypothesis that maternal 3,3-Dimethyl-1-butanol (DMB, a TMA inhibitor) therapy prevents 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) exposure-induced hypertension in adult offspring relevant to alterations of gut microbiota-derived metabolites, the mediation of aryl hydrocarbon receptor (AHR) signaling, and the renin-angiotensin system (RAS). Pregnant Sprague-Dawley rats were given weekly oral dose of TCDD 200 ng/kg for four doses (T), 1% DMB in drinking water (D), TCDD + DMB (TD), or vehicle (C) in pregnancy and lactation periods. Male progeny (n = 8/group) were sacrificed at the age of 12 weeks. Perinatal TCDD exposure caused hypertension in adult male offspring coinciding with reduced α-diversity, increased the Firmicutes to Bacteroidetes ratio, less abundant beneficial bacteria, impaired SCFA receptors’ expression, the activation of AHR signaling, and the aberrant activation of the RAS. Treatment with DMB during pregnancy and lactation rescued hypertension induced by perinatal TCDD exposure. This was accompanied by reshaping gut microbiota, mediating TMA-TMAO metabolic pathway, increasing acetic acid and its receptors, and restoring the AHR and RAS pathway. Our data provide new insights into the therapeutic potential of DMB, a microbiome-based metabolite treatment, for the prevention of hypertension of developmental origins.
Chien-Ning Hsu; Chih-Yao Hou; Chien-Te Lee; Guo-Ping Chang-Chien; Sufan Lin; You-Lin Tain. Maternal 3,3-Dimethyl-1-Butanol Therapy Protects Adult Male Rat Offspring against Hypertension Programmed by Perinatal TCDD Exposure. Nutrients 2021, 13, 3041 .
AMA StyleChien-Ning Hsu, Chih-Yao Hou, Chien-Te Lee, Guo-Ping Chang-Chien, Sufan Lin, You-Lin Tain. Maternal 3,3-Dimethyl-1-Butanol Therapy Protects Adult Male Rat Offspring against Hypertension Programmed by Perinatal TCDD Exposure. Nutrients. 2021; 13 (9):3041.
Chicago/Turabian StyleChien-Ning Hsu; Chih-Yao Hou; Chien-Te Lee; Guo-Ping Chang-Chien; Sufan Lin; You-Lin Tain. 2021. "Maternal 3,3-Dimethyl-1-Butanol Therapy Protects Adult Male Rat Offspring against Hypertension Programmed by Perinatal TCDD Exposure." Nutrients 13, no. 9: 3041.
Exposure to environmental chemicals during pregnancy and lactation is a contributing factor in gut microbiota dysbiosis and linked to programming of hypertension. 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), the most toxic dioxin, induces toxic effects by mediating aryl hydrocarbon receptor (AHR). Resveratrol, a potent antioxidant with prebiotic properties, can possess high affinity for AHR and protect against TCDD-activated AHR attack. We examined whether perinatal resveratrol therapy prevents offspring hypertension programmed by maternal TCDD exposure and whether its beneficial effects are related to reshaping gut microbiota and antagonizing AHR-mediated T helper 17 (TH17) cells responses using a maternal TCDD exposure rat model. Pregnant Sprague-Dawley rats were given a weekly oral dose of TCDD 200 ng/kg for four doses (T), 50 mg/L of resveratrol in drinking water (CR), TCDD + resveratrol (TR), or vehicle (C) in pregnancy and lactation periods. Male offspring (n = 7–8/group) were sacrificed at the age of 12 weeks. Perinatal TCDD exposure caused elevated blood pressure in adult male offspring, which resveratrol supplementation prevented. Additionally, the TCDD-induced programming of hypertension is coincided with the activation of AHR signaling, TH17-induced renal inflammation, and alterations of gut microbiota compositions. Conversely, TCDD-mediated induction of AHR signaling and TH17 responses were restored by maternal resveratrol supplementation. Furthermore, maternal resveratrol supplementation prevented the programming of hypertension and was related to increased genera Bacteroides, ASF356, and Lachnoclostridium. Taken together, these results suggest that the interplay between gut microbiota, AHR-mediated TH17 responses, and renal inflammation in the gut and kidneys may play an important role in the action of resveratrol against TCDD-induced programming of hypertension.
Chien-Ning Hsu; Chih-Hsing Hung; Chih-Yao Hou; Chi-I. Chang; You-Lin Tain. Perinatal Resveratrol Therapy to Dioxin-Exposed Dams Prevents the Programming of Hypertension in Adult Rat Offspring. Antioxidants 2021, 10, 1393 .
AMA StyleChien-Ning Hsu, Chih-Hsing Hung, Chih-Yao Hou, Chi-I. Chang, You-Lin Tain. Perinatal Resveratrol Therapy to Dioxin-Exposed Dams Prevents the Programming of Hypertension in Adult Rat Offspring. Antioxidants. 2021; 10 (9):1393.
Chicago/Turabian StyleChien-Ning Hsu; Chih-Hsing Hung; Chih-Yao Hou; Chi-I. Chang; You-Lin Tain. 2021. "Perinatal Resveratrol Therapy to Dioxin-Exposed Dams Prevents the Programming of Hypertension in Adult Rat Offspring." Antioxidants 10, no. 9: 1393.
Lemon (Citrus limon) has antioxidant, immunoregulatory, and blood lipid-lowering properties. This study aimed to determine the effect of the lemon fermented product (LFP) which is lemon fermented with Lactobacillus OPC1 to prevent obesity. The inhibition of lipid accumulation in 3T3-L1 adipocytes is examined using a Wistar rat model fed a high-fat diet to verify the anti-obesity efficacy and mechanism of LFP. Here, it was observed that LFP reduced cell proliferation and inhibited the lipid accumulation (8.3%) of 3T3-L1 adipocytes. Additionally, LFP reduced body weight (9.7%) and fat tissue weight (25.7%) of rats; reduced serum TG (17.0%), FFA (17.9%), glucose (29.3%) and ketone body (6.8%); and increased serum HDL-C (17.6%) and lipase activity (17.8%). LFP regulated the mRNA expression of genes related to lipid metabolism (PPARγ, C/EBPα, SREBP-1c, HSL, ATGL, FAS, and AMPK). Therefore, LFP reduces body weight and lipid accumulation by regulating the mRNA expression of genes related to lipid metabolism. Overall, our results implicate LFP as a potential dietary supplement for the prevention of obesity.
Chih-Chung Wu; Yu-Wen Huang; Chih-Yao Hou; Ya-Ting Chen; Cheng-Di Dong; Chiu-Wen Chen; Reeta-Rani Singhania; Jie-Yin Leang; Shu-Ling Hsieh. The Anti-Obesity Effects of Lemon Fermented Products in 3T3-L1 Preadipocytes and in a Rat Model with High-Calorie Diet-Induced Obesity. Nutrients 2021, 13, 2809 .
AMA StyleChih-Chung Wu, Yu-Wen Huang, Chih-Yao Hou, Ya-Ting Chen, Cheng-Di Dong, Chiu-Wen Chen, Reeta-Rani Singhania, Jie-Yin Leang, Shu-Ling Hsieh. The Anti-Obesity Effects of Lemon Fermented Products in 3T3-L1 Preadipocytes and in a Rat Model with High-Calorie Diet-Induced Obesity. Nutrients. 2021; 13 (8):2809.
Chicago/Turabian StyleChih-Chung Wu; Yu-Wen Huang; Chih-Yao Hou; Ya-Ting Chen; Cheng-Di Dong; Chiu-Wen Chen; Reeta-Rani Singhania; Jie-Yin Leang; Shu-Ling Hsieh. 2021. "The Anti-Obesity Effects of Lemon Fermented Products in 3T3-L1 Preadipocytes and in a Rat Model with High-Calorie Diet-Induced Obesity." Nutrients 13, no. 8: 2809.
Melatonin, a signaling hormone with pleiotropic biofunctions, has shown health benefits. Trimethylamine-N-oxide (TMAO) and asymmetric dimethylarginine (ADMA) are uremic toxins involved in the development of hypertension. TMAO originates from trimethylamine (TMA), a gut microbial product. ADMA is an endogenous nitric oxide (NO) synthase inhibitor. We examined whether melatonin therapy could prevent hypertension and kidney disease by mediating gut microbiota-derived metabolites and the NO pathway using an adenine-induced chronic kidney disease (CKD) young rat model. Six-week-old young Sprague Dawley rats of both sexes were fed a regular diet (C group), a diet supplemented with 0.5% adenine (CKD group), or adenine plus 0.01% melatonin in their drinking water (CKD + M group) for three weeks (N = 8/group). Adenine-fed rats developed renal dysfunction, hypertension, renal hypertrophy and increased uremic toxin levels of TMAO and ADMA. Melatonin therapy prevented hypertension in both sexes and attenuated kidney injury in males. Melatonin reversed the changes to the plasma TMAO-to-TMA ratio induced by CKD in both sexes. Besides, the protective effects of melatonin were associated with restoration of gut microbiota alterations, including increased α-diversity, and enhancement of the abundance of the phylum Proteobacteria and the genus Roseburia in male rats. Melatonin therapy also partially prevented the increases in ADMA in male CKD rats. Melatonin sex-specifically protected young rats against hypertension and kidney injury induced by CKD. The results of this study contribute toward a greater understanding of the interaction between melatonin, gut microbiota-derived metabolites, and the NO pathway that is behind CKD, which will help to prevent CKD-related disorders in children.
Chien-Ning Hsu; Hung-Wei Yang; Chih-Yao Hou; Guo-Ping Chang-Chien; Sufan Lin; You-Lin Tain. Melatonin Prevents Chronic Kidney Disease-Induced Hypertension in Young Rat Treated with Adenine: Implications of Gut Microbiota-Derived Metabolites. Antioxidants 2021, 10, 1211 .
AMA StyleChien-Ning Hsu, Hung-Wei Yang, Chih-Yao Hou, Guo-Ping Chang-Chien, Sufan Lin, You-Lin Tain. Melatonin Prevents Chronic Kidney Disease-Induced Hypertension in Young Rat Treated with Adenine: Implications of Gut Microbiota-Derived Metabolites. Antioxidants. 2021; 10 (8):1211.
Chicago/Turabian StyleChien-Ning Hsu; Hung-Wei Yang; Chih-Yao Hou; Guo-Ping Chang-Chien; Sufan Lin; You-Lin Tain. 2021. "Melatonin Prevents Chronic Kidney Disease-Induced Hypertension in Young Rat Treated with Adenine: Implications of Gut Microbiota-Derived Metabolites." Antioxidants 10, no. 8: 1211.
Consumer awareness and distaste towards both bacterial and chemical contaminations on food items have been increasing in recent years. Non-thermal plasma (NTP) is a cutting-edge technology which has been shown to effectively inactivate bacteria on the treated foods. Although the general NTP with a single plasma jet is appropriate for the continuous operation process, it suffers limitations due to its smaller scanning area. Here, a novel NTP device with a double rotary nozzle jet system was utilized, which could treat an area instead of a point. The shell eggs inoculated with Salmonella enterica serotype Enteritidis (SE) were placed on a moving platform under the double rotary nozzle jet system. The efficacy of the NTP treatment on microbial decontamination was evaluated by testing a total of 26 combinations of operating parameters consisting of various plasma power (150, 180, 210 W), argon flow rate (10, 15, 20 slm), repetition of the moving platform (4, 6, 8 times), and speed of the moving platform (5, 10 mm/s). Although significantly higher SE reduction (p 4 log CFU/egg of SE reduction and significantly better sensory properties than commercially washed eggs (p<0.05). Additionally, SEM analysis revealed that NTP treatment with combination T resulted in less damage to egg cuticles compared to commercially washed eggs. This novel NTP device offers an efficient antibacterial activity under shorter exposure time (30 s), smaller argon flow rate (15 slm), and lower power (180 W) without adversely affecting the overall quality of the treated eggs. Therefore, this NTP device equipped with the double rotary jet system possesses a potential solution for future industrial applications.
Chia-Min Lin; Samuel Herianto; Hsiu-Ling Chen; Yen-Chuan Chiu; Chih-Yao Hou. The application of a novel non-thermal plasma device with double rotary plasma jets for inactivation of Salmonella Enteritidis on shell eggs and its effects on sensory properties. International Journal of Food Microbiology 2021, 355, 109332 .
AMA StyleChia-Min Lin, Samuel Herianto, Hsiu-Ling Chen, Yen-Chuan Chiu, Chih-Yao Hou. The application of a novel non-thermal plasma device with double rotary plasma jets for inactivation of Salmonella Enteritidis on shell eggs and its effects on sensory properties. International Journal of Food Microbiology. 2021; 355 ():109332.
Chicago/Turabian StyleChia-Min Lin; Samuel Herianto; Hsiu-Ling Chen; Yen-Chuan Chiu; Chih-Yao Hou. 2021. "The application of a novel non-thermal plasma device with double rotary plasma jets for inactivation of Salmonella Enteritidis on shell eggs and its effects on sensory properties." International Journal of Food Microbiology 355, no. : 109332.
Clostridioides difficile infection (CDI) is a large intestine disease caused by toxins produced by the spore-forming bacterium C. difficile, which belongs to Gram-positive bacillus. Using antibiotics treatment disturbances in the gut microbiota and toxins produced by C. difficile disrupt the intestinal barrier. Some evidence indicates fecal microbiota transplantation and probiotics may decrease the risk of CDI recurrence. This study aimed to evaluate the efficacy of fermented mango by using the lactic acid bacteria Lactobacillus acidophilus and develop innovative products in the form of fermented mango jam. L. acidophilus-fermented mango products inhibited the growth of C. difficile while promoting the growth of next-generation probiotic Faecalibacterium prausnitzii. Both supernatant and precipitate of mango-fermented products prevented cell death in gut enterocyte-like Caco-2 cells against C. difficile infection. Mango-fermented products also protected gut barrier function by elevating the expression of tight junction proteins. Moreover, L. acidophilus-fermented mango jam with high hydrostatic pressure treatment had favorable textural characteristics and sensory quality.
Bao-Hong Lee; Wei-Hsuan Hsu; Hao-Yuan Chien; Chih-Yao Hou; Ya-Ting Hsu; You-Zuo Chen; She-Ching Wu. Applications of Lactobacillus acidophilus-Fermented Mango Protected Clostridioides difficile Infection and Developed as an Innovative Probiotic Jam. Foods 2021, 10, 1631 .
AMA StyleBao-Hong Lee, Wei-Hsuan Hsu, Hao-Yuan Chien, Chih-Yao Hou, Ya-Ting Hsu, You-Zuo Chen, She-Ching Wu. Applications of Lactobacillus acidophilus-Fermented Mango Protected Clostridioides difficile Infection and Developed as an Innovative Probiotic Jam. Foods. 2021; 10 (7):1631.
Chicago/Turabian StyleBao-Hong Lee; Wei-Hsuan Hsu; Hao-Yuan Chien; Chih-Yao Hou; Ya-Ting Hsu; You-Zuo Chen; She-Ching Wu. 2021. "Applications of Lactobacillus acidophilus-Fermented Mango Protected Clostridioides difficile Infection and Developed as an Innovative Probiotic Jam." Foods 10, no. 7: 1631.
Cardiovascular diseases (CVDs) can originate from early life. Accumulating evidence suggests that gut microbiota in early life is linked to CVDs in later life. Gut microbiota-targeted therapy has gained significant importance in recent decades for its health-promoting role in the prevention (rather than just treatment) of CVDs. Thus far, available gut microbiota-based treatment modalities used as reprogramming interventions include probiotics, prebiotics, and postbiotics. The purpose of this review is, first, to highlight current studies that link dysbiotic gut microbiota to the developmental origins of CVD. This is followed by a summary of the connections between the gut microbiota and CVD behind cardiovascular programming, such as short chain fatty acids (SCFAs) and their receptors, trimethylamine-N-oxide (TMAO), uremic toxins, and aryl hydrocarbon receptor (AhR), and the renin-angiotensin system (RAS). This review also presents an overview of how gut microbiota-targeted reprogramming interventions can prevent the developmental origins of CVD from animal studies. Overall, this review reveals that recent advances in gut microbiota-targeted therapy might provide the answers to reduce the global burden of CVDs. Still, additional studies will be needed to put research findings into practice.
Chien-Ning Hsu; Chih-Yao Hou; Wei-Hsuan Hsu; You-Lin Tain. Cardiovascular Diseases of Developmental Origins: Preventive Aspects of Gut Microbiota-Targeted Therapy. Nutrients 2021, 13, 2290 .
AMA StyleChien-Ning Hsu, Chih-Yao Hou, Wei-Hsuan Hsu, You-Lin Tain. Cardiovascular Diseases of Developmental Origins: Preventive Aspects of Gut Microbiota-Targeted Therapy. Nutrients. 2021; 13 (7):2290.
Chicago/Turabian StyleChien-Ning Hsu; Chih-Yao Hou; Wei-Hsuan Hsu; You-Lin Tain. 2021. "Cardiovascular Diseases of Developmental Origins: Preventive Aspects of Gut Microbiota-Targeted Therapy." Nutrients 13, no. 7: 2290.
Resveratrol butyrate esters (RBE) are derivatives of resveratrol (RSV) and butyric acid and exhibit biological activity similar to that of RSV but with higher bioavailability. The aim of this study was designed as an animal experiment to explore the effects of RBE on the serum biochemistry, and fat deposits in the offspring rats exposed to bisphenol A (BPA), along with the growth and decline of gut microbiota. We constructed an animal model of perinatal Bisphenol A (BPA) exposure to observe the effects of RBE supplementation on obesity, blood lipids, and intestinal microbiota in female offspring rats. Perinatal exposure to BPA led to weight gain, lipid accumulation, high levels of blood lipids, and deterioration of intestinal microbiota in female offspring rats. RBE supplementation reduced the weight gain and lipid accumulation caused by BPA, optimised the levels of blood lipids, significantly reduced the Firmicutes/Bacteroidetes (F/B) ratio, and increased and decreased the abundance of S24-7 and Lactobacillus, respectively. The analysis of faecal short-chain fatty acid (SCFA) levels revealed that BPA exposure increased the faecal concentration of acetate, which could be reduced via RBE supplementation. However, the faecal concentrations of propionate and butyrate were not only significantly lower than that of acetate, but also did not significantly change in response to BPA exposure or RBE supplementation. Hence, RBE can suppress BPA-induced obesity in female offspring rats, and it demonstrates excellent modulatory activity on intestinal microbiota, with potential applications in perinatological research.
Ming-Kuei Shih; You-Lin Tain; Yu-Wei Chen; Wei-Hsuan Hsu; Yao-Tsung Yeh; Sam Chang; Jin-Xian Liao; Chih-Yao Hou. Resveratrol Butyrate Esters Inhibit Obesity Caused by Perinatal Exposure to Bisphenol A in Female Offspring Rats. Molecules 2021, 26, 4010 .
AMA StyleMing-Kuei Shih, You-Lin Tain, Yu-Wei Chen, Wei-Hsuan Hsu, Yao-Tsung Yeh, Sam Chang, Jin-Xian Liao, Chih-Yao Hou. Resveratrol Butyrate Esters Inhibit Obesity Caused by Perinatal Exposure to Bisphenol A in Female Offspring Rats. Molecules. 2021; 26 (13):4010.
Chicago/Turabian StyleMing-Kuei Shih; You-Lin Tain; Yu-Wei Chen; Wei-Hsuan Hsu; Yao-Tsung Yeh; Sam Chang; Jin-Xian Liao; Chih-Yao Hou. 2021. "Resveratrol Butyrate Esters Inhibit Obesity Caused by Perinatal Exposure to Bisphenol A in Female Offspring Rats." Molecules 26, no. 13: 4010.
In this study, a preservation package that can extend the shelf life of Agaricus bisporus was developed using plasma modification combined with low-density polyethylene (LDPE), collagen (COL), and carboxymethyl cellulose (CMC). Out results showed that the selectivity of LDPE to gas can be controlled by plasma modification combined with coating of different concentrations of CMC and COL. Packaging test results applied to A. bisporus showed that 3% and 5% of CMC and COL did not significantly inhibit polyphenol oxidase and β-1,3-glucanase, indicating no significant effect on structural integrity and oxidative browning. The use of 0.5% and 1.0% CMC and COL can effectively inhibit the polyphenol oxidase and β-1,3-glucanase activity of A. bisporus, leading to improved effects in browning inhibition and structural integrity maintenance. P-1.0COL can effectively maintain gas composition in the package (carbon dioxide: 10–15% and oxygen: 8–15%) and catalase activity during storage, thereby reducing the oxidative damage caused by respiration of A. bisporus. The current study confirmed that the use of plasma modification technology combined with 1.0% COL can be used in preservation packaging by regulating the respiration of A. bisporus, thus extending its shelf life from 7 to 21 days.
Chao-Kai Chang; Kuan-Chen Cheng; Chih-Yao Hou; Yi-Shan Wu; Chang-Wei Hsieh. Development of Active Packaging to Extend the Shelf Life of Agaricus bisporus by Using Plasma Technology. Polymers 2021, 13, 2120 .
AMA StyleChao-Kai Chang, Kuan-Chen Cheng, Chih-Yao Hou, Yi-Shan Wu, Chang-Wei Hsieh. Development of Active Packaging to Extend the Shelf Life of Agaricus bisporus by Using Plasma Technology. Polymers. 2021; 13 (13):2120.
Chicago/Turabian StyleChao-Kai Chang; Kuan-Chen Cheng; Chih-Yao Hou; Yi-Shan Wu; Chang-Wei Hsieh. 2021. "Development of Active Packaging to Extend the Shelf Life of Agaricus bisporus by Using Plasma Technology." Polymers 13, no. 13: 2120.
A pasteurized liquid egg leads to protein denaturation and degradation of processing properties, whereas non-pasteurized eggs may have food safety risks. If the negative impact of the pasteurization process on liquid eggs can be reduced, for example, the loss of stability and foamability, companies will be willing to purchase pasteurized eggs, thereby reducing food safety risks. Therefore, in this study, specific hydrolyzation conditions were used to produce egg white hydrolysate (EWH) with a lower molecular mass of amino acid and peptide fragments, and the effects of various concentration of EWH refilling on pasteurized liquid egg properties were investigated. The results showed that up to 30.1% of EWH was hydrolyzed by protease A and papain. Adding 1% (w/w) EWH can improve the negative charge potential value, surface tension, viscosity, and weight loss analysis of the sample. In addition, the cake structure and the appearance was acceptable to consumers. Therefore, to ensure its efficient use in the baking industry and considering the cost and stability, 1% (w/w) EWH was chosen as the best concentration.
Hen-Yo Ho; Jhih-Ying Ciou; Yi-Ting Qiu; Shu-Ling Hsieh; Ming-Kuei Shih; Min-Hung Chen; Chao-Wen Tu; Chang-Wei Hsieh; Chih-Yao Hou. Improvement of Foaming Characteristics and Stability of Sterilized Liquid Egg with Egg White Hydrolysate (EWH). Foods 2021, 10, 1326 .
AMA StyleHen-Yo Ho, Jhih-Ying Ciou, Yi-Ting Qiu, Shu-Ling Hsieh, Ming-Kuei Shih, Min-Hung Chen, Chao-Wen Tu, Chang-Wei Hsieh, Chih-Yao Hou. Improvement of Foaming Characteristics and Stability of Sterilized Liquid Egg with Egg White Hydrolysate (EWH). Foods. 2021; 10 (6):1326.
Chicago/Turabian StyleHen-Yo Ho; Jhih-Ying Ciou; Yi-Ting Qiu; Shu-Ling Hsieh; Ming-Kuei Shih; Min-Hung Chen; Chao-Wen Tu; Chang-Wei Hsieh; Chih-Yao Hou. 2021. "Improvement of Foaming Characteristics and Stability of Sterilized Liquid Egg with Egg White Hydrolysate (EWH)." Foods 10, no. 6: 1326.
Mango peels are usually discarded as waste; however, they contain phytochemicals and could provide functional properties to food and promote human health. This study aimed to determine the optimal lactic acid bacteria for fermentation of mango peel and evaluate the effect of mango peel on neuronal protection in Neuron-2A cells against amyloid beta (Aβ) treatment (50 μM). Mango peel can be fermented by different lactic acid bacteria species. Lactobacillus acidophilus (BCRC14079)-fermented mango peel produced the highest concentration of lactic acid bacteria (exceeding 108 CFU/mL). Mango peel and fermented mango peel extracts upregulated brain-derived neurotrophic factor (BDNF) expression for 1.74-fold in Neuron-2A cells. Furthermore, mango peel fermented products attenuated oxidative stress in Aβ-treated neural cells by 27%. Extracts of L. acidophilus (BCRC14079)-fermented mango peel treatment decreased Aβ accumulation and attenuated the increase of subG1 caused by Aβ induction in Neuron-2A cells. In conclusion, L. acidophilus (BCRC14079)-fermented mango peel acts as a novel neuronal protective product by inhibiting oxidative stress and increasing BDNF expression in neural cells.
Bao-Hong Lee; Wei-Hsuan Hsu; Chih-Yao Hou; Hao-Yuan Chien; She-Ching Wu. The Protection of Lactic Acid Bacteria Fermented-Mango Peel against Neuronal Damage Induced by Amyloid-Beta. Molecules 2021, 26, 3503 .
AMA StyleBao-Hong Lee, Wei-Hsuan Hsu, Chih-Yao Hou, Hao-Yuan Chien, She-Ching Wu. The Protection of Lactic Acid Bacteria Fermented-Mango Peel against Neuronal Damage Induced by Amyloid-Beta. Molecules. 2021; 26 (12):3503.
Chicago/Turabian StyleBao-Hong Lee; Wei-Hsuan Hsu; Chih-Yao Hou; Hao-Yuan Chien; She-Ching Wu. 2021. "The Protection of Lactic Acid Bacteria Fermented-Mango Peel against Neuronal Damage Induced by Amyloid-Beta." Molecules 26, no. 12: 3503.
This study aims to determine the physicochemical quality of seagrape (Caulerpa lentillifera) as a freshness label for products cultivated in different seasons. The applied post-harvest storage experiments compared between, within and without seawater that led to oxidative stress conditions. Water content, malondialdehyde (MDA) compound, total phenolic content (TPC), and chlorophyll content were observed at 0, 3, 6, and 9 days of storage. The storage without seawater showed sharper quality reductions by reaching 20–40% of water loss, 70–90% of MDA production, 15–25% of TPC reduction, and 40–60% of total chlorophyll degradation. The storage within seawater showed lower quality reductions due to the specific growth rates still reaching 5–10%. This study found that the greater the physicochemical quality, the slower the decomposition rates of the stored seagrape during storage. Therefore, the seagrapes’ obvious discoloration occurred earlier in winter, followed by summer and spring. Kinetics of chlorophyll degradation on seagrape in different seasons meet different order-reactions during storage. Furthermore, alternating current electric field (ACEF) treatment with 125 kV/m of intensity for 60 min can lower the spring seagrapes’ physicochemical quality by reaching 10–30% of inhibition, resulting in the shelf-life extension for up to 12 days of post-harvest storage.
Andi Sulaimana; Chao-Kai Chang; Chih-Yao Hou; Bara Yudhistira; Fuangfah Punthi; Chun-Ta Lung; Kuan-Chen Cheng; Shella Santoso; Chang-Wei Hsieh. Effect of Oxidative Stress on Physicochemical Quality of Taiwanese Seagrape (Caulerpa lentillifera) with the Application of Alternating Current Electric Field (ACEF) during Post-Harvest Storage. Processes 2021, 9, 1011 .
AMA StyleAndi Sulaimana, Chao-Kai Chang, Chih-Yao Hou, Bara Yudhistira, Fuangfah Punthi, Chun-Ta Lung, Kuan-Chen Cheng, Shella Santoso, Chang-Wei Hsieh. Effect of Oxidative Stress on Physicochemical Quality of Taiwanese Seagrape (Caulerpa lentillifera) with the Application of Alternating Current Electric Field (ACEF) during Post-Harvest Storage. Processes. 2021; 9 (6):1011.
Chicago/Turabian StyleAndi Sulaimana; Chao-Kai Chang; Chih-Yao Hou; Bara Yudhistira; Fuangfah Punthi; Chun-Ta Lung; Kuan-Chen Cheng; Shella Santoso; Chang-Wei Hsieh. 2021. "Effect of Oxidative Stress on Physicochemical Quality of Taiwanese Seagrape (Caulerpa lentillifera) with the Application of Alternating Current Electric Field (ACEF) during Post-Harvest Storage." Processes 9, no. 6: 1011.
Toxic heavy metals accumulate in crops from the environment through different routes and may interfere with biochemical reactions in humans, causing serious health consequences. Plasma technology has been assessed for the promotion of seed germination and plant growth in several past studies. Therefore, the aim of the present study was to evaluate whether the growth rate of plants can be increased with the application of non-thermal plasma, as well as to reduce the accumulation of heavy metals in leafy vegetables (water spinach). In this study, several kinds of plasma treatments were applied, such as treatment on the seeds (PTS + NTW), irrigation water (NTS + PAW) or both (PTS + PAW). The results of the study showed that the heavy metals accumulated in water spinach were affected by the heavy metals available in the soil. The bioconcentration factor (BCF) of Cd in water spinach decreased from 0.864 to 0.543 after plasma treatment in seed or irrigating water, while the BCF of Pb was low and did not show any significant changes. Therefore, the results suggest that plasma treatment may suppress Cd absorption, but not for Pb. In this study, plasma treatment did not help to improve the product yield of water spinach planted in Cd-added soil. In the future, fertilizers can be used to supply nutrients that are not provided by plasma-activated water to support the growth of water spinach.
Chih-Yao Hou; Ting-Khai Kong; Chia-Min Lin; Hsiu-Ling Chen. The Effects of Plasma-Activated Water on Heavy Metals Accumulation in Water Spinach. Applied Sciences 2021, 11, 5304 .
AMA StyleChih-Yao Hou, Ting-Khai Kong, Chia-Min Lin, Hsiu-Ling Chen. The Effects of Plasma-Activated Water on Heavy Metals Accumulation in Water Spinach. Applied Sciences. 2021; 11 (11):5304.
Chicago/Turabian StyleChih-Yao Hou; Ting-Khai Kong; Chia-Min Lin; Hsiu-Ling Chen. 2021. "The Effects of Plasma-Activated Water on Heavy Metals Accumulation in Water Spinach." Applied Sciences 11, no. 11: 5304.
Resveratrol can affect the physiology or biochemistry of offspring in the maternal–fetal animal model. However, it exhibits low bioavailability in humans and animals. Fifteen-week SD pregnant female rats were orally administered bisphenol A (BPA) and/or resveratrol butyrate ester (RBE), and the male offspring rats (n = 4–8 per group) were evaluated. The results show that RBE treatment (BPA + R30) compared with the BPA group can reduce the damage caused by BPA (p< 0.05). RBE enhanced the expression of selected genes and induced extramedullary hematopoiesis and mononuclear cell infiltration. RBE increased the abundance of S24-7 and Adlercreutzia in the intestines of the male offspring rats, as well as the concentrations of short-chain fatty acids (SCFAs) in the feces. RBE also increased the antioxidant capacity of the liver by inducing Nrf2, promoting the expression of HO-1, SOD, and CAT. It also increased the concentration of intestinal SCFAs, enhancing the barrier formed by intestinal cells, thereby preventing BPA-induced metabolic disruption in the male offspring rats, and reduced liver inflammation. This study identified a potential mechanism underlying the protective effects of RBE against the liver damage caused by BPA exposure during the peri-pregnancy period, and the influence of the gut microbiota on the gut–liver axis in the offspring.
Jin-Xian Liao; Yu-Wei Chen; Ming-Kuei Shih; You-Lin Tain; Yao-Tsung Yeh; Min-Hsi Chiu; Sam Chang; Chih-Yao Hou. Resveratrol Butyrate Esters Inhibit BPA-Induced Liver Damage in Male Offspring Rats by Modulating Antioxidant Capacity and Gut Microbiota. International Journal of Molecular Sciences 2021, 22, 5273 .
AMA StyleJin-Xian Liao, Yu-Wei Chen, Ming-Kuei Shih, You-Lin Tain, Yao-Tsung Yeh, Min-Hsi Chiu, Sam Chang, Chih-Yao Hou. Resveratrol Butyrate Esters Inhibit BPA-Induced Liver Damage in Male Offspring Rats by Modulating Antioxidant Capacity and Gut Microbiota. International Journal of Molecular Sciences. 2021; 22 (10):5273.
Chicago/Turabian StyleJin-Xian Liao; Yu-Wei Chen; Ming-Kuei Shih; You-Lin Tain; Yao-Tsung Yeh; Min-Hsi Chiu; Sam Chang; Chih-Yao Hou. 2021. "Resveratrol Butyrate Esters Inhibit BPA-Induced Liver Damage in Male Offspring Rats by Modulating Antioxidant Capacity and Gut Microbiota." International Journal of Molecular Sciences 22, no. 10: 5273.
The increase in the incidence of cardiovascular diseases (CVDs) and kidney disease has stimulated research for strategies that could prevent, rather than just treat, both interconnected disorders. Resveratrol, a polyphenolic compound with pleiotropic biofunctions, has shown health benefits. Emerging epidemiological data supports that early life environmental insults are regarded as increased risks of developing CVDs and kidney disease in adulthood. Conversely, both disorders could be reversed or postponed by shifting interventions from adulthood to earlier stage by so-called reprogramming. The purpose of this review is first to highlight current epidemiological studies linking cardiovascular and renal programming to resulting CVD and kidney disease of developmental origins. This will be followed by a summary of how resveratrol could exert a positive influence on CVDs and kidney disease. This review also presents an overview of the evidence documenting resveratrol as a reprogramming agent to protect against CVD and kidney disease of developmental origins from animal studies and to outline the advances in understanding the underlying molecular mechanisms. Overall, this review reveals the need for future research to further clarify the reprogramming effects of resveratrol before clinical translation.
Chien-Ning Hsu; Chih-Yao Hou; You-Lin Tain. Preventive Aspects of Early Resveratrol Supplementation in Cardiovascular and Kidney Disease of Developmental Origins. International Journal of Molecular Sciences 2021, 22, 4210 .
AMA StyleChien-Ning Hsu, Chih-Yao Hou, You-Lin Tain. Preventive Aspects of Early Resveratrol Supplementation in Cardiovascular and Kidney Disease of Developmental Origins. International Journal of Molecular Sciences. 2021; 22 (8):4210.
Chicago/Turabian StyleChien-Ning Hsu; Chih-Yao Hou; You-Lin Tain. 2021. "Preventive Aspects of Early Resveratrol Supplementation in Cardiovascular and Kidney Disease of Developmental Origins." International Journal of Molecular Sciences 22, no. 8: 4210.
To expand the applications and enhance the stability and bioactivity of resveratrol (RE), and to simultaneously include the potential health benefits of short chain fatty acids (SCFA) esters of RE were prepared by Steglich reactions with acetic, propionic, and butyric acids, respectively. RE and the esterified RE-SCFA products (including RAE, RPE, and RBE) were analyzed using nuclear magnetic resonance (NMR), Fourier-transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), differential thermal analysis (DTA), and liquid chromatography–mass spectrometry (LC–MS). The FTIR and 13C NMR spectra of the esterified products included ester-characteristic peaks at 1751 cm−1 and 171 ppm, respectively. Moreover, the peaks in the range of 1700 to 1600 cm−1 in the FTIR spectra of the esterified products indicated that the esterification of RE-SCFA was successful. The TGA results revealed that the RE-SCFA esters decomposed at lower temperatures than RE. The peaks in the LC–MS profiles of the esterified products indicated the formation of mono- and diesters, and the calculated monoester synthesis rates ranged between 45.81 and 49.64%. The RE esters inhibited the Cu2+-induced low-density lipoprotein oxidation reaction, exhibited antioxidant activity in bulk oil, and effectively inhibited the hydroxyl radical-induced DNA scission. Moreover, the RE-SCFA esters had better hydrogen peroxide scavenging activity than RE. Our results are the first in the literature to successfully including short chain fatty acids in the esters of resveratrol, and the products could be used as a functional food ingredient in processed foods or can be used as dietary supplements to promote health.
You-Lin Tain; Sam Chang; Jin-Xian Liao; Yu-Wei Chen; Hung-Tse Huang; Yu-Lun Li; Chih-Yao Hou. Synthesis of Short-Chain-Fatty-Acid Resveratrol Esters and Their Antioxidant Properties. Antioxidants 2021, 10, 420 .
AMA StyleYou-Lin Tain, Sam Chang, Jin-Xian Liao, Yu-Wei Chen, Hung-Tse Huang, Yu-Lun Li, Chih-Yao Hou. Synthesis of Short-Chain-Fatty-Acid Resveratrol Esters and Their Antioxidant Properties. Antioxidants. 2021; 10 (3):420.
Chicago/Turabian StyleYou-Lin Tain; Sam Chang; Jin-Xian Liao; Yu-Wei Chen; Hung-Tse Huang; Yu-Lun Li; Chih-Yao Hou. 2021. "Synthesis of Short-Chain-Fatty-Acid Resveratrol Esters and Their Antioxidant Properties." Antioxidants 10, no. 3: 420.
Gut microbiota-derived metabolites, in particular short chain fatty acids (SCFAs) and their receptors, are linked to hypertension. Fructose and antibiotics are commonly used worldwide, and they have a negative impact on the gut microbiota. Our previous study revealed that maternal high-fructose (HF) diet-induced hypertension in adult offspring is relevant to altered gut microbiome and its metabolites. We, therefore, intended to examine whether minocycline administration during pregnancy and lactation may further affect blood pressure (BP) programmed by maternal HF intake via mediating gut microbiota and SCFAs. Pregnant Sprague-Dawley rats received a normal diet or diet containing 60% fructose throughout pregnancy and lactation periods. Additionally, pregnant dams received minocycline (50 mg/kg/day) via oral gavage or a vehicle during pregnancy and lactation periods. Four groups of male offspring were studied (n = 8 per group): normal diet (ND), high-fructose diet (HF), normal diet + minocycline (NDM), and HF + minocycline (HFM). Male offspring were killed at 12 weeks of age. We observed that the HF diet and minocycline administration, both individually and together, causes the elevation of BP in adult male offspring, while there is no synergistic effect between them. Four groups displayed distinct enterotypes. Minocycline treatment leads to an increase in the F/B ratio, but decreased abundance of genera Lactobacillus, Ruminococcus, and Odoribacter. Additionally, minocycline treatment decreases plasma acetic acid and butyric acid levels. Hypertension programmed by maternal HF diet plus minocycline exposure is related to the increased expression of several SCFA receptors. Moreover, minocycline- and HF-induced hypertension, individually or together, is associated with the aberrant activation of the renin–angiotensin system (RAS). Conclusively, our results provide a new insight into the support of gut microbiota and its metabolite SCAFs in the developmental programming of hypertension and cast new light on the role of RAS in this process, which will help prevent hypertension programmed by maternal high-fructose and antibiotic exposure.
Chien-Ning Hsu; Julie Chan; Kay Wu; Hong-Ren Yu; Wei-Chia Lee; Chih-Yao Hou; You-Lin Tain. Altered Gut Microbiota and Its Metabolites in Hypertension of Developmental Origins: Exploring Differences between Fructose and Antibiotics Exposure. International Journal of Molecular Sciences 2021, 22, 2674 .
AMA StyleChien-Ning Hsu, Julie Chan, Kay Wu, Hong-Ren Yu, Wei-Chia Lee, Chih-Yao Hou, You-Lin Tain. Altered Gut Microbiota and Its Metabolites in Hypertension of Developmental Origins: Exploring Differences between Fructose and Antibiotics Exposure. International Journal of Molecular Sciences. 2021; 22 (5):2674.
Chicago/Turabian StyleChien-Ning Hsu; Julie Chan; Kay Wu; Hong-Ren Yu; Wei-Chia Lee; Chih-Yao Hou; You-Lin Tain. 2021. "Altered Gut Microbiota and Its Metabolites in Hypertension of Developmental Origins: Exploring Differences between Fructose and Antibiotics Exposure." International Journal of Molecular Sciences 22, no. 5: 2674.
This study aimed to develop an antimicrobial active packaging using carboxymethyl cellulose (CMC) or collagen (COL) as carriers of cinnamaldehyde (CNMA) coating on plasma-treated low density polyethylene (LDPE) for application on tilapia fillet packaging. COL carriers exhibited stronger antimicrobial and antioxidant activities than no carrier and CMC. The films also showed good physical and mechanical properties after coating. The results on tilapia preservation analysis showed that the COL with 6% CNMA slowed the total plate count and growth of Vibrio parahaemolyticus by about 1.82 log colony-forming units (CFU)/g and 1.76 log CFU/g compared to the control at day 14. All active films were better than Control in delaying the production of volatile basic nitrogen and thiobarbituric acid during storage whilst simultaneously maintaining organoleptic properties. Based on results, the antimicrobial active films significantly prolonged the shelf life of tilapia fillets to 3 days compared with the Control group. The COL with 6% CNMA may potentially play an effective role in maintaining and preserving the microbial qualities of tilapia fillets.
Xin-Jie Loke; Chao-Kai Chang; Chih-Yao Hou; Kuan-Chen Cheng; Chang-Wei Hsieh. Plasma-treated polyethylene coated with polysaccharide and protein containing cinnamaldehyde for active packaging films and applications on tilapia (Orechromis niloticus) fillet preservation. Food Control 2021, 125, 108016 .
AMA StyleXin-Jie Loke, Chao-Kai Chang, Chih-Yao Hou, Kuan-Chen Cheng, Chang-Wei Hsieh. Plasma-treated polyethylene coated with polysaccharide and protein containing cinnamaldehyde for active packaging films and applications on tilapia (Orechromis niloticus) fillet preservation. Food Control. 2021; 125 ():108016.
Chicago/Turabian StyleXin-Jie Loke; Chao-Kai Chang; Chih-Yao Hou; Kuan-Chen Cheng; Chang-Wei Hsieh. 2021. "Plasma-treated polyethylene coated with polysaccharide and protein containing cinnamaldehyde for active packaging films and applications on tilapia (Orechromis niloticus) fillet preservation." Food Control 125, no. : 108016.
Concerns related to foodborne pathogens such as Salmonella enterica serovar Enteritidis (SE) necessitate the decontamination of eggs. Multiple studies have successfully demonstrated the use of non-thermal plasma (NTP) technology in inactivating SE on artificially inoculated shell eggs. However, the NTP devices abovementioned were operated in the batch mode and small scale. The present study is the first attempt to evaluate the effectiveness of a large-scale device using NTP for egg decontamination. The designed NTP was operated at 400 W (power), 30 slm (Ar flow rate), 5 and 10 eggs/min (speed of conveyor belt), and 4 cm (distance between the plasma jets and egg samples). Conducting a two-round treatment for the egg samples at 400 W-30 slm-5 eggs/min-4 cm achieved the highest reduction of SE populations (3.57 log CFU/egg). SEM observation revealed less damage on the surface of the plasma-treated eggs than the commercial-washed eggs. Overall, NTP treatment can maintain the freshness quality and sensory characteristics until 15-day storage. This study demonstrated a large-scale NTP system was able to effectively achieve a significant reduction of pathogenic SE without adversely affecting egg quality properties. These results suggested the readiness of this NTP device for industrial applications.
Chia-Min Lin; Samuel Herianto; Shih-Ming Syu; Cian-Huei Song; Hsiu-Ling Chen; Chih-Yao Hou. Applying a large-scale device using non-thermal plasma for microbial decontamination on shell eggs and its effects on the sensory characteristics. LWT 2021, 142, 111067 .
AMA StyleChia-Min Lin, Samuel Herianto, Shih-Ming Syu, Cian-Huei Song, Hsiu-Ling Chen, Chih-Yao Hou. Applying a large-scale device using non-thermal plasma for microbial decontamination on shell eggs and its effects on the sensory characteristics. LWT. 2021; 142 ():111067.
Chicago/Turabian StyleChia-Min Lin; Samuel Herianto; Shih-Ming Syu; Cian-Huei Song; Hsiu-Ling Chen; Chih-Yao Hou. 2021. "Applying a large-scale device using non-thermal plasma for microbial decontamination on shell eggs and its effects on the sensory characteristics." LWT 142, no. : 111067.
Sesame seed oil is a healthy food used to enhance food flavor. This research was to identify the influences of oil properties on the quality of powders produced with sesame oil through spray drying on variables of the blending ratio of the sesame oil and the concentration of the core material. The encapsulation efficiency levels of the sesame oil encapsulated with maltodextrin and hydroxypropyl methylcellulose through spray drying ranged from 23.59‐43.78%, with the encapsulation efficiency being significantly influenced by the oil concentration and blending ratio. The analysis further showed that the moisture content, solubility and bulk/tapped densities were notable influenced by the blending ratio of the sesame oil. The bulk density and powder solubility had an inverse relationship, with fat usually exhibiting hydrophobic behavior and thus hindering the water absorption capacity of powders. hygroscopicity of powders can strongly influence their incorporation into food system with diverse chemical and physical properties.
Jhih‐Ying Ciou; Kai‐Min Yang; Chih‐Yao Hou; Jia‐Yin You. The physicochemical properties of spray‐dried sesame powder with different blending ratios. Journal of Food Processing and Preservation 2021, 45, 1 .
AMA StyleJhih‐Ying Ciou, Kai‐Min Yang, Chih‐Yao Hou, Jia‐Yin You. The physicochemical properties of spray‐dried sesame powder with different blending ratios. Journal of Food Processing and Preservation. 2021; 45 (3):1.
Chicago/Turabian StyleJhih‐Ying Ciou; Kai‐Min Yang; Chih‐Yao Hou; Jia‐Yin You. 2021. "The physicochemical properties of spray‐dried sesame powder with different blending ratios." Journal of Food Processing and Preservation 45, no. 3: 1.