Bu çalışmada fibromiyalji sendromunda (FMS) Sistemik İmmün-İnflamasyon İndeksi (SII) ile kan parametreleri arasındaki ilişki değerlendirilerek hastalık aktivitesinin belirlenmesi amaçlanmıştır. Çalışmaya Ocak 2021 ve Ocak 2022 tarihleri arasında Fizik Tedavi ve Rehabilitasyon Kliniğine başvuran 109 FMS hastası ve 82 sağlıklı kontrol dahil edilmiştir. Yaş, cinsiyet, C-reaktif protein (CRP), eritrosit sedimentasyon hızı (ESR), beyaz kan hücresi (WBC), hemoglobin (HGB), ortalama korpusküler hacim (MCV), ortalama korpusküler hemoglobin (MCH), ortalama korpusküler hemoglobin konsantrasyonu (MCHC), kırmızı hücre dağılımı (RDW), trombosit dağılım genişliği (PDW), ortalama trombosit hacmi (MPV), trombosit (PLT), trombosit kriteri (PCT), lenfosit (LYM), monosit (MONO), nötrofil (NEU) düzeyleri hastane bilgi sisteminden retrospektif olarak taranmıştır. Trombosit-lenfosit oranı (PLR), nötrofil-lenfosit oranı (NLR), monosit-lenfosit oranı (MLR) ve sistemik immün-inflamasyon indeksi (SII) hesaplanmıştır. CRP ve ESR düzeyleri FMS hastalarında sağlıklı kontrollere göre daha yüksekti ancak değer aralığının dışında değildi (p<0,001). PDW (p<0,001), HGB (p<0,001), MCV (p<0,001), MCH (p<0,001) ve MCHC (p=0,02) düzeyleri hastalarda sağlıklı gruba göre daha düşüktü. Hasta ve kontrol grupları arasında yaş, WBC, NEU, LYM, MONO, RDW, MPV, PLT, PCT, SII, PLR, NLR ve MLR değerleri açısından istatistiksel olarak anlamlı fark yoktu. FMS hastalarında WBC düzeyleri ile SSS değerleri arasında (r=0,2; p=0,005) ve lenfosit düzeyleri ile WPI değerleri arasında (r=0,2; p=0,01) anlamlı pozitif korelasyon bulunmuştur. Çalışma sonuçlarına göre, SII'nin FMS'de belirleyici bir rolü yoktur, ancak hastalığın tanıda yararlı olabilecek bazı inflamatuvar bileşenleri vardır ve daha ayrıntılı çalışmalara ihtiyaç vardır.

Background and Objectives: The effects of nicotine on wound healing and flap survival remain controversial. While smoking is associated with increased flap complications, emerging evidence suggests that nicotine may exert dose- and duration-dependent effects. This study aimed to investigate how short-term, low-dose nicotine affects flap survival and to evaluate its potential role in oxidative stress and ferroptosis. Materials and Methods: Twelve adult male BALB/c mice were randomly assigned to either a control group or a nicotine-treated group, with six mice in each group. Nicotine, at a dose of 2 mg/kg, was given subcutaneously for fourteen days before flap surgery. This treatment continued for seven days after the surgery. A dorsal random-pattern skin flap model was established in all animals. The flap necrosis area was assessed on postoperative day 7 using planimetric analysis. Serum and tissue glutathione peroxidase 4 (GPX4) levels and serum malondialdehyde (MDA) levels were measured. Histopathological evaluation included epithelialization, microvascular density, neovascularization, fibroblast density, and inflammatory cell infiltration. Results: Nicotine treatment significantly reduced flap necrosis compared with the control group. Histopathological analysis demonstrated significantly increased epithelialization and microvascular density in the nicotine-treated group, whereas no significant differences were observed in neovascularization, fibroblast density, or inflammatory cell infiltration. Biochemical analyses revealed no significant differences in serum or tissue GPX4 levels or serum MDA levels between the groups. Conclusions: Short-term nicotine administration enhanced flap survival, correlating with increased epithelialization and microvascular density, while not significantly affecting markers of oxidative stress or ferroptosis. These results imply that the positive impact of nicotine on flap viability might be largely attributable to vascular processes rather than pathways involving oxidative stress.

Prediabetes is a condition characterized by insulin resistance, impaired glucose tolerance, and metabolic imbalance, occurring before the onset of diabetes. Angiopoietin-like protein 4 (ANGPTL4), known for its role in lipid metabolism and energy regulation, has been proposed as a potential biomarker in the progression of prediabetes due to its regulatory role in lipid metabolism and systemic metabolic homeostasis. However, data on ANGPTL4 levels in prediabetic individuals and their association with metabolic parameters remain limited. This study aimed to investigate ANGPTL4 levels in a rat model of prediabetes and assess its relationship with key metabolic indicators. Male Wistar rats (6–8 weeks old) were randomly assigned to two groups: prediabetic (n=24) and healthy controls (n=22). The prediabetic group was fed a high-fat diet for 12 weeks, while the control group received a standard diet. Blood samples were collected, and ANGPTL4 levels were measured using the ELISA method. Metabolic parameters were also evaluated, including glucose, insulin, HbA1c, lipid profile (total cholesterol, LDL, HDL, triglycerides), HOMA-IR, and HOMA-β. ANGPTL4 levels were significantly elevated in the prediabetic group compared to controls (p

Eyelid reconstruction may be necessary after eyelid burns, trauma, or tumor resections. The limited availability of tarsoconjunctival tissue has led to the use of various grafts, including hard palate mucosa, lip mucosa, cartilage grafts, and acellular dermal matrix. This study aimed to evaluate the experimental use of deep fascia grafts in eyelid reconstruction, hypothesizing that they can provide adequate structural and physiological support when covered by conjunctival epithelium. An experimental study was conducted on 16 rabbits, divided into two groups: a graft group (n=8) and a control group (n=8). A 1x1 cm defect was created in the posterior lamella of the lower eyelid in all animals. The defect in the graft group was repaired with a deep fascia graft from the anterior thigh, while defects in the control group were left to secondary healing. Macroscopic outcomes included hematoma, eye irritation, conjunctivitis, and scleral show. Microscopic outcomes included inflammation, fibrosis, vascularization, conjunctival epithelialization, and goblet cell presence. Macroscopic changes were monitored for one month. Microscopic evaluations of harvested tissues were performed by a pathologist, and statistical analyses determined significance. In the graft group, no scleral show was observed, while it was evident in the control group. Microscopic examination revealed statistically significant differences in inflammation and vascularization between groups. The deep fascia graft demonstrated sufficient support, compatibility with conjunctival epithelium, and absence of ocular irritation. These results suggest that deep fascia may serve as a viable alternative for posterior lamella reconstruction.