Bacillus licheniformis Mb1, a thermophilic strain isolated from the Yungas rainforest in northwestern Argentina, was analyzed through genomic and experimental approaches to explore its biotechnological potential. Phylogenomic analysis confirmed its close relationship with B. licheniformis reference strains. The genome revealed multiple genes associated with hydrolytic, oxidative, carbohydrate-active, and polyester-degrading activities, indicating a wide enzymatic capacity. Experimental assays demonstrated strong extracellular hydrolytic activities and efficient degradation of bis(2-hydroxyethyl) terephthalate (BHET), a key polyethylene terephthalate (PET) intermediate. In liquid cultures with 3 mg/mL BHET, B. licheniformis Mb1 achieved 99.9% depletion within four days, with transient BHET dimer accumulation and progressive terephthalic acid (TPA) production, reaching 1.17 mg/mL after 15 days. Mono (2-hydroxyethyl) terephthalate (MHET) and vanillic acid were not detected. Complete BHET and dimer degradation suggests the presence of versatile hydrolases acting on short-chain polyester intermediates. Sequence and molecular docking analyses identified a BHETase-like carboxylesterase as the main enzyme candidate, featuring a truncated lidC region that generates a more open catalytic cleft. This structural trait, not previously reported in bacterial BHETases, enables the accommodation of bulkier substrates such as BHET dimer. These findings highlight B. licheniformis Mb1 as a promising biocatalyst for polyester depolymerization and a valuable microbial resource for future enzyme discovery and plastic bioremediation strategies.

The accumulation of polyethylene terephthalate (PET) in the environment demands efficient microbial strategies for its degradation. This study evaluates the biodegradation potential of Schizophyllum commune BNT39 toward bis(2-hydroxyethyl) terephthalate (BHET), a major PET intermediate, and PET itself. Clear halos on BHET-agar plates indicated extracellular hydrolytic activity. In liquid culture, thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) analyses revealed a three-phase degradation profile characterized by rapid BHET hydrolysis, transient dimer accumulation, and subsequent conversion to terephthalic acid (TPA). BHET was reduced by approximately 96% within seven days, while TPA accumulation reached 0.8 mg/mL after 30 days of incubation. Although PET degradation was limited, TPA was consistently detected as the principal product, with no BHET or MHET intermediates. To explore strategies for enhancing enzymatic activity, apple-derived cutin, PET, BHET, and polycaprolactone (PCL) were tested as inducers. Cutin markedly stimulated extracellular enzyme production, suggesting activation of cutinase-like enzymes. Overall, S. commune BNT39 demonstrates the ability to transform PET-related substrates, with cutin emerging as a promising natural stimulant to enhance enzymatic depolymerization. Future studies should focus on enzyme purification, activity profiling, and reaction optimization near PET’s glass transition temperature, where the polymer becomes more accessible for enzymatic attack.

Glyphosate [N-(phosphonomethyl)glycine] is the most widely used herbicide worldwide, and its environmental persistence has prompted increasing interest in microbial processes that may contribute to its dissipation. This study evaluated a collection of 15 soil-derived actinobacterial strains for plant growth-promoting traits, extracellular enzymatic activities, glyphosate tolerance, and glyphosate removal under nutrient-sufficient and phosphate-starved conditions. Herbicide tolerance evaluated on agar plates was widespread across the collection, with all strains sustaining growth at 10 and 50 g L⁻¹ of glyphosate. Under nutrient-sufficient conditions glyphosate removal remained limited, with maximum values of 16.15 ± 2.08% (Streptomyces sp. Con7.16) and 15.34 ± 2.89% (Streptomyces sp. Z38). In contrast, prior phosphate starvation markedly enhanced removal efficiency, reaching 42.21 ± 3.59% in Streptomyces sp. Z38 and 39.46 ± 1.94% in Streptomyces sp. Con7.16. Transmission electron microscopy coupled with X-ray microanalysis in the selected Streptomyces sp. Z38 revealed starvation-associated depletion of intracellular polyphosphate granules, followed by partial replenishment when glyphosate was supplied as the sole phosphorus source, consistent with indirect evidence of glyphosate-derived phosphorus acquisition. Genome mining of Streptomyces sp. Z38 identified candidate genes potentially consistent with a non-canonical, C-P lyase-independent phosphonate utilization route; however, these assignments are based exclusively on bioinformatic evidence and require experimental validation. Collectively, these findings indicate that phosphate limitation enhances glyphosate removal in the selected actinobacteria, and the physiological and genomic data are consistent with a starvation-triggered shift toward alternative phosphorus scavenging strategies. Because this strain is intended for future phytoremediation applications in glyphosate-contaminated agricultural soils, elucidating the underlying phosphorus dynamics is essential for anticipating its functional behavior and environmental relevance.