The interface between the conductive polymer, polyaniline (PAn-Cl), and gold, platinum, or an interceding layer of electrodeposited platinum on gold or platinum, markedly influences the apparent electrical properties and the electronic to ionic transition in physiological buffers. Polyester-supported, sputter-deposited gold and platinum thin films were laser patterned to yield co-planar Thin Film Electrodes (TFEs) suitable for platinization and deposition of PAn-Cl nanofibers. Electrodeposition of platinum from chloroplatinic acid (50 mC cm⁻²) onto gold produced larger feature sizes and larger surface roughness (23.5 nm) when compared to platinization of platinum (15.2 nm) and both similarly reduced interfacial impedance in water and physiologically relevant buffers, PBS and HEPES. UV-Vis characterization produced absorption edges (DI water 2.36 eV, PBS 2.64 eV, and HEPES 2.66 eV) reflective of the ionic strength of the medium. Thin films (23 ± 2 μm) of PAn-Cl nanofibers were deposited onto Au, Pt, Au|Pt, Pt|Pt TFEs and each characterized by Electrical Impedance Spectroscopy (EIS) over the range 10⁶–10⁻¹ Hz at RT in air, DI water, PBS, and HEPES buffers and by multiple scan rate cyclic voltammetry (MSRCV) in PBS. Platinized gold and platinized platinum decorated with PAn-Cl behaved quite differently in these test environments confirming a role for the contacting surface roughness/nano-topography in influencing apparent electrical properties. Equivalent circuit modeling of EIS data revealed a modified Randles circuit (R(QR)) of low chi-square values (<0.05) that rationalized the capacitance and membrane resistance and confirmed that platinization of gold served to increase the PAn-Cl apparent resistance while platinization of platinum served to decrease the PAn-Cl apparent resistance.

The formation of hybrid bioactive and inherently conductive constructs of composites formed from polyaniline-polyacrylamidomethylpropane sulfonic acid (PAn-PAAMPSA) nanomaterials (0.00–10.0 wt%) within poly(2-hydroxy ethyl methacrylate-co-N-{Tris(hydroxymethyl)methyl} acrylamide)-co-polyethyleneglycol methacrylate) p(HEMA-co-HMMA-co-PEGMA) hydrogels was made possible using microlithographic fabrication and 3-D printing. Hybrid constructs formed by combining a non-conductive base (0.00 wt% PAn-PAAMPSA) and electroconductive (ECH) (varying wt% PAn-PAAMPSA) hydrogels using these two production techniques were directly compared. Hydrogels were electrically characterized using two-point probe resistivity and electrochemical impedance spectroscopy. Results show that incorporation of >0.10 wt% PAn-PAAMPSA within the base hydrogel matrices was enough to achieve percolation and high conductivity with a membrane resistance (R_M) of 2140 Ω and 87.9 Ω for base (0.00 wt%) and ECH (10.0 wt%), respectively. UV-vis spectroscopy of electroconductive hydrogels indicated a bandgap of 2.8 eV that was measurable at concentrations of >0.10 wt% PAn-PAAMPSA. Both base and electroconductive hydrogels supported the attachment and growth of NIH/3T3 fibroblast cells. When the base hydrogel was rendered bioactive by the inclusion of collagen (>200 µg/mL), it also supported the attachment, but not the differentiation, of PC-12 neural progenitor cells.