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Prof. Bożena Cukrowska
The Children's Memorial Health Institute

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0 Allergy
0 Celiac Disease
0 Mucosal Immunology
0 Probiotics
0 microbiome

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Celiac Disease
Allergy
microbiome
Probiotics

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Journal article
Published: 18 May 2021 in Cancers
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Continuous activation of the immune system inside a tissue can lead to remodelling of the tissue structure and creation of a specific microenvironment, such as during the tumour development. Chronic inflammation is a central player in stimulating changes that alter the tissue stroma and can lead to fibrotic evolution. In the colon mucosa, regulatory mechanisms, including TGF-β1, avoid damaging inflammation in front of the continuous challenge by the intestinal microbiome. Inducing either DSS colitis or AOM colorectal carcinogenesis in AVN-Wistar rats, we evaluated at one month after the end of each treatment whether immunological changes and remodelling of the collagen scaffold were already in development. At this time point, we found in both models a general downregulation of pro-inflammatory cytokines and even of TGF-β1, but not of IL-6. Moreover, we demonstrated by multi-photon microscopy the simultaneously presence of pro-fibrotic remodelling of the collagen scaffold, with measurable changes in comparison to the control mucosa. The scaffold was significantly modified depending on the type of induced stimulation. These results suggest that at one month after the end of the DSS or AOM inductions, a smouldering inflammation is present in both induced conditions, since the pro-inflammatory cytokines still exceed, in proportion, the local homeostatic regulation of which TGF-β1 is a part (inflammatory threshold). Such an inflammation appears sufficient to sustain remodelling of the collagen scaffold that may be taken as a possible pathological marker for revealing pre-neoplastic inflammation.

ACS Style

Fabián Čaja; Dmitry Stakheev; Oleksandr Chernyavskiy; Lucie Kubinová; Jiří Křížan; Jiří Dvořák; Pavel Rossmann; Renata Štěpánková; Peter Makovický; Pavol Makovický; Veronika Vymetalková; Pavel Souček; Pavel Vodička; L’Udmila Vodičková; Miroslav Levý; Luca Vannucci. Local Immune Changes in Early Stages of Inflammation and Carcinogenesis Correlate with the Collagen Scaffold Changes of the Colon Mucosa. Cancers 2021, 13, 2463 .

AMA Style

Fabián Čaja, Dmitry Stakheev, Oleksandr Chernyavskiy, Lucie Kubinová, Jiří Křížan, Jiří Dvořák, Pavel Rossmann, Renata Štěpánková, Peter Makovický, Pavol Makovický, Veronika Vymetalková, Pavel Souček, Pavel Vodička, L’Udmila Vodičková, Miroslav Levý, Luca Vannucci. Local Immune Changes in Early Stages of Inflammation and Carcinogenesis Correlate with the Collagen Scaffold Changes of the Colon Mucosa. Cancers. 2021; 13 (10):2463.

Chicago/Turabian Style

Fabián Čaja; Dmitry Stakheev; Oleksandr Chernyavskiy; Lucie Kubinová; Jiří Křížan; Jiří Dvořák; Pavel Rossmann; Renata Štěpánková; Peter Makovický; Pavol Makovický; Veronika Vymetalková; Pavel Souček; Pavel Vodička; L’Udmila Vodičková; Miroslav Levý; Luca Vannucci. 2021. "Local Immune Changes in Early Stages of Inflammation and Carcinogenesis Correlate with the Collagen Scaffold Changes of the Colon Mucosa." Cancers 13, no. 10: 2463.

Journal article
Published: 01 April 2021 in Nutrients
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Probiotics seem to have promising effects in the prevention and treatment of allergic conditions including atopic dermatitis (AD) and food allergy. The purpose of this multicenter randomized placebo-controlled trial was to evaluate the effectiveness of a probiotic preparation comprising Lactobacillus rhamnosus ŁOCK 0900, Lactobacillus rhamnosus ŁOCK 0908, and Lactobacillus casei ŁOCK 0918 in children under 2 years of age with AD and a cow’s milk protein (CMP) allergy. The study enrolled 151 children, who—apart from being treated with a CMP elimination diet—were randomized to receive the probiotic preparation at a daily dose of 109 bacteria or a placebo for three months, with a subsequent nine-month follow-up. The primary outcomes included changes in AD symptom severity assessed with the scoring AD (SCORAD) index and in the proportion of children with symptom improvement (a SCORAD score decreased by at least 30% in comparison with that at baseline). After the three-month intervention, both the probiotic and placebo groups showed a significant (p < 0.0001) decrease in SCORAD scores, which was maintained nine months later. The percentage of children who showed improvement was significantly higher in the probiotic than in the placebo group (odds ratio (OR) 2.56; 95% confidence interval (CI) 1.13–5.8; p = 0.012) after three months. Probiotics induced SCORAD improvement mainly in allergen sensitized patients (OR 6.03; 95% CI 1.85–19.67, p = 0.001), but this positive effect was not observed after nine months. The results showed that the mixture of probiotic ŁOCK strains offers benefits for children with AD and CMP allergy. Further research is necessary to assess the effect of probiotic supplementation on the development of immune tolerance (NCT04738565).

ACS Style

Bożena Cukrowska; Aldona Ceregra; Elżbieta Maciorkowska; Barbara Surowska; Maria Zegadło-Mylik; Ewa Konopka; Ilona Trojanowska; Magdalena Zakrzewska; Joanna Bierła; Mateusz Zakrzewski; Ewelina Kanarek; Ilona Motyl. The Effectiveness of Probiotic Lactobacillus rhamnosus and Lactobacillus casei Strains in Children with Atopic Dermatitis and Cow’s Milk Protein Allergy: A Multicenter, Randomized, Double Blind, Placebo Controlled Study. Nutrients 2021, 13, 1169 .

AMA Style

Bożena Cukrowska, Aldona Ceregra, Elżbieta Maciorkowska, Barbara Surowska, Maria Zegadło-Mylik, Ewa Konopka, Ilona Trojanowska, Magdalena Zakrzewska, Joanna Bierła, Mateusz Zakrzewski, Ewelina Kanarek, Ilona Motyl. The Effectiveness of Probiotic Lactobacillus rhamnosus and Lactobacillus casei Strains in Children with Atopic Dermatitis and Cow’s Milk Protein Allergy: A Multicenter, Randomized, Double Blind, Placebo Controlled Study. Nutrients. 2021; 13 (4):1169.

Chicago/Turabian Style

Bożena Cukrowska; Aldona Ceregra; Elżbieta Maciorkowska; Barbara Surowska; Maria Zegadło-Mylik; Ewa Konopka; Ilona Trojanowska; Magdalena Zakrzewska; Joanna Bierła; Mateusz Zakrzewski; Ewelina Kanarek; Ilona Motyl. 2021. "The Effectiveness of Probiotic Lactobacillus rhamnosus and Lactobacillus casei Strains in Children with Atopic Dermatitis and Cow’s Milk Protein Allergy: A Multicenter, Randomized, Double Blind, Placebo Controlled Study." Nutrients 13, no. 4: 1169.

Review
Published: 11 March 2021 in Medicina
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Cancer, bacteria, and immunity relationships are much-debated topics in the last decade. Microbiome’s importance for metabolic and immunologic modulation of the organism adaptation and responses has become progressively evident, and models to study these relationships, especially about carcinogenesis, have acquired primary importance. The availability of germ-free (GF) animals, i.e., animals born and maintained under completely sterile conditions avoiding the microbiome development offers a unique tool to investigate the role that bacteria can have in carcinogenesis and tumor development. The comparison between GF animals with the conventional (CV) counterpart with microbiome can help to evidence conditions and mechanisms directly involving bacterial activities in the modulation of carcinogenesis processes. Here, we review the literature about spontaneous cancer and cancer modeling in GF animals since the early studies, trying to offer a practical overview on the argument.

ACS Style

Rajbardhan Mishra; Lenka Rajsiglová; Pavol Lukáč; Paolo Tenti; Peter Šima; Fabián Čaja; Luca Vannucci. Spontaneous and Induced Tumors in Germ-Free Animals: A General Review. Medicina 2021, 57, 260 .

AMA Style

Rajbardhan Mishra, Lenka Rajsiglová, Pavol Lukáč, Paolo Tenti, Peter Šima, Fabián Čaja, Luca Vannucci. Spontaneous and Induced Tumors in Germ-Free Animals: A General Review. Medicina. 2021; 57 (3):260.

Chicago/Turabian Style

Rajbardhan Mishra; Lenka Rajsiglová; Pavol Lukáč; Paolo Tenti; Peter Šima; Fabián Čaja; Luca Vannucci. 2021. "Spontaneous and Induced Tumors in Germ-Free Animals: A General Review." Medicina 57, no. 3: 260.

Journal article
Published: 26 February 2021 in Nutrients
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The aim of this randomized double-blind placebo-controlled study was to evaluate the effectiveness and safety of multi-strain probiotic in adults with diarrhea-predominant irritable bowel syndrome (IBS-D). The patients were randomized to receive a mixture of Lactobacillus, Bifidobacterium, and Streptococcus thermophilus strains or placebo for eight weeks. Primary endpoints included changes in symptom severity and improvement assessed with the IBS Severity Scoring System (IBS-SSS) and Global Improvement Scale (IBS-GIS). The probiotic in comparison with placebo significantly improved the IBS symptom severity (the change of total IBS-SSS score from baseline −165.8 ± 78.9 in the probiotic group and −105.6 ± 60.2 in the placebo group, p = 0.005) and in the specific scores related to the severity of pain (p = 0.015) and the quality of life (p = 0.016) after eight weeks of intervention. The probiotic group indicated an improvement in symptoms with the use of the IBS-GIS compared with the placebo group after four (p = 0.04) and eight weeks (p = 0.003). The occurrence of adverse events did not differ between study groups. In conclusion, the multi-strain probiotic intervention resulted in a significant improvement in IBS symptoms evaluated with the use of both IBS-SSS and IBS-GIS scales. The results suggest that the studied probiotic preparation is well tolerated and safe and can offer benefits for patients with IBS-D. (registration number in Clinicaltrials.gov NCT 04662957).

ACS Style

Barbara Skrzydło-Radomańska; Beata Prozorow-Król; Halina Cichoż-Lach; Emilia Majsiak; Joanna Bierła; Ewelina Kanarek; Agnieszka Sowińska; Bożena Cukrowska. The Effectiveness and Safety of Multi-Strain Probiotic Preparation in Patients with Diarrhea-Predominant Irritable Bowel Syndrome: A Randomized Controlled Study. Nutrients 2021, 13, 756 .

AMA Style

Barbara Skrzydło-Radomańska, Beata Prozorow-Król, Halina Cichoż-Lach, Emilia Majsiak, Joanna Bierła, Ewelina Kanarek, Agnieszka Sowińska, Bożena Cukrowska. The Effectiveness and Safety of Multi-Strain Probiotic Preparation in Patients with Diarrhea-Predominant Irritable Bowel Syndrome: A Randomized Controlled Study. Nutrients. 2021; 13 (3):756.

Chicago/Turabian Style

Barbara Skrzydło-Radomańska; Beata Prozorow-Król; Halina Cichoż-Lach; Emilia Majsiak; Joanna Bierła; Ewelina Kanarek; Agnieszka Sowińska; Bożena Cukrowska. 2021. "The Effectiveness and Safety of Multi-Strain Probiotic Preparation in Patients with Diarrhea-Predominant Irritable Bowel Syndrome: A Randomized Controlled Study." Nutrients 13, no. 3: 756.

Journal article
Published: 05 July 2020 in Nutrients
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The purpose of the randomized double-blind placebo-controlled trial was to assess the effectiveness of synbiotic preparation containing probiotic Lactobacillus rhamnosus FloraActive™ 19070-2, Lactobacillus acidophilus DSMZ 32418, Bifidobacterium lactis DSMZ 32269, Bifidobacterium longum DSMZ 32946, Bifidobacterium bifidum DSMZ 32403 and fructooligosaccharides in adult patients with diarrhea-dominant IBS (IBS-D). The study included eighty patients with moderate and severe IBS-D who were randomized to receive synbiotics or placebo for eight weeks. Finally, a total of sixty-eight patients finished the study. The primary endpoints included the assessment of the symptoms’ severity with IBS symptom severity scale (IBS-SSS), an improvement of IBS global symptoms with Global Improvement Scale (IBS-GIS) and adequate relief of symptoms after four and eight weeks of therapy. Secondary endpoints, which were collected by telephone interviewers three times a week included the assessment of individual IBS symptoms and adverse events. Synbiotic treatment in comparison to placebo significantly improved IBS-GIS (p = 0.043), and IBS-SSS score inducing a decrease in the total IBS-SSS (p = 0.042) and in domain-specific scores related to flatulence (p = 0.028) and bowel habit (p = 0.028) after four and eight weeks. Patients treated with synbiotics reported in weekly observations a significant amelioration in a feeling of incomplete bowel movements, flatulence, pain, stool pressure and diarrheal stools compared to those receiving placebo. There were no differences in adverse events between both groups. Concluding, the multi-strain synbiotic preparation was associated with a significant improvement in symptoms in IBS-D patients and was well-tolerated. These results suggest that the use of synbiotics offers a benefit for IBS-D patients. [Clinicaltrials.gov NCT04206410 registered 20 December 2019].

ACS Style

Barbara Skrzydło-Radomańska; Beata Prozorow-Król; Halina Cichoż-Lach; Emilia Majsiak; Joanna B. Bierła; Wojciech Kosikowski; Mariusz Szczerbiński; Jesper Gantzel; Bożena Cukrowska. The Effectiveness of Synbiotic Preparation Containing Lactobacillus and Bifidobacterium Probiotic Strains and Short Chain Fructooligosaccharides in Patients with Diarrhea Predominant Irritable Bowel Syndrome—A Randomized Double-Blind, Placebo-Controlled Study. Nutrients 2020, 12, 1999 .

AMA Style

Barbara Skrzydło-Radomańska, Beata Prozorow-Król, Halina Cichoż-Lach, Emilia Majsiak, Joanna B. Bierła, Wojciech Kosikowski, Mariusz Szczerbiński, Jesper Gantzel, Bożena Cukrowska. The Effectiveness of Synbiotic Preparation Containing Lactobacillus and Bifidobacterium Probiotic Strains and Short Chain Fructooligosaccharides in Patients with Diarrhea Predominant Irritable Bowel Syndrome—A Randomized Double-Blind, Placebo-Controlled Study. Nutrients. 2020; 12 (7):1999.

Chicago/Turabian Style

Barbara Skrzydło-Radomańska; Beata Prozorow-Król; Halina Cichoż-Lach; Emilia Majsiak; Joanna B. Bierła; Wojciech Kosikowski; Mariusz Szczerbiński; Jesper Gantzel; Bożena Cukrowska. 2020. "The Effectiveness of Synbiotic Preparation Containing Lactobacillus and Bifidobacterium Probiotic Strains and Short Chain Fructooligosaccharides in Patients with Diarrhea Predominant Irritable Bowel Syndrome—A Randomized Double-Blind, Placebo-Controlled Study." Nutrients 12, no. 7: 1999.

Review
Published: 29 March 2020 in Nutrients
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The increase in allergy prevalence observed in recent decades may be a consequence of early intestinal dysbiosis. The intestinal microbiota is formed in the first 1000 days of life, when it is particularly sensitive to various factors, such as the composition of the mother’s microbiota, type of delivery, infant’s diet, number of siblings, contact with animals, and antibiotic therapy. Breastfeeding and vaginal birth favorably affect the formation of an infant’s intestinal microbiota and protect against allergy development. The intestinal microbiota of these infants is characterized by an early dominance of Bifidobacterium, which may have a significant impact on the development of immune tolerance. Bifidobacterium breve is a species commonly isolated from the intestines of healthy breastfed infants and from human milk. This review outlines the most important environmental factors affecting microbiota formation and the importance of Bifidobacterium species (with a particular emphasis on Bifidobacterium breve) in microbiota modulation towards anti-allergic processes. In addition, we present the concept, which assumes that infant formulas containing specific probiotic Bifidobacterium breve strains and prebiotic oligosaccharides may be useful in allergy management in non-breastfed infants.

ACS Style

Bożena Cukrowska; Joanna B. Bierła; Magdalena Zakrzewska; Mark Klukowski; Elżbieta Maciorkowska. The Relationship between the Infant Gut Microbiota and Allergy. The Role of Bifidobacterium breve and Prebiotic Oligosaccharides in the Activation of Anti-Allergic Mechanisms in Early Life. Nutrients 2020, 12, 946 .

AMA Style

Bożena Cukrowska, Joanna B. Bierła, Magdalena Zakrzewska, Mark Klukowski, Elżbieta Maciorkowska. The Relationship between the Infant Gut Microbiota and Allergy. The Role of Bifidobacterium breve and Prebiotic Oligosaccharides in the Activation of Anti-Allergic Mechanisms in Early Life. Nutrients. 2020; 12 (4):946.

Chicago/Turabian Style

Bożena Cukrowska; Joanna B. Bierła; Magdalena Zakrzewska; Mark Klukowski; Elżbieta Maciorkowska. 2020. "The Relationship between the Infant Gut Microbiota and Allergy. The Role of Bifidobacterium breve and Prebiotic Oligosaccharides in the Activation of Anti-Allergic Mechanisms in Early Life." Nutrients 12, no. 4: 946.

Research article
Published: 27 March 2020 in Gastroenterology Research and Practice
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Introduction and Objectives. Celiac disease (CD) is a complex autoimmune disorder occurring in genetically susceptible individuals. There is limited data on the impact of gluten-free diet (GFD) on the risk of developing cardiovascular diseases. Hereby, we present our study regarding the impact of treatment with GFD on the biochemical risk factors of atherosclerosis. Material and Methods. 277 patients with CD from 7 Polish clinics were enrolled in the study (210 children treated for at least 5 years and 67 children included in the study on the day of CD diagnosis and observed for 1 year on a GFD). We obtained selected clinical data, and we assessed lipid profile, apolipoproteins (A1, B, and E), lipoprotein (a), homocysteine, as well as antioxidants (folic and uric acid), and high-sensitivity CRP (hsCRP) for all patients. The compliance to GFD was verified using anti-transglutaminase antibodies and deamidated gliadin peptide antibodies. As a reference group, the data of 95 healthy children recruited for another project was used, for which we had the results of selected parameters. Results. We found significantly lower concentrations of total cholesterol, lipoprotein LDL-C, apolipoproteins A1 and B, as well as hCRP in all children with CD. We showed decreased level (5 years. Moreover, we showed significant decrease of folic acid level already after 1 year of a GFD (12 vs. 5.6 ng/mL; p < 0.001 ). We also found significant negative correlation of z -score body mass index (BMI) with HDL and APOA1 level ( r = − 0.33 ; p = 0.015 and r = − 0.28 ; p = 0.038 , respectively) and modest positive correlation of z -score BMI with atherogenic factor of total cholesterol-HDL ratio and LDL-HDL ratio ( r = 0.40 ; p = 0.002 and r = 0.36 ; p = 0.006 , respectively). Analysis of physical activity showed an increase in the insulin levels with inactivity ( r = 0.36 ; p = 0.0025 ). We also found positive correlation of the sleep duration with the adiponectin level ( r = 0.41 ; p = 0.011 ). Conclusions. In children with CD treated with a GFD, decreased level of folic acid together with increased BMI, sedentary behavior, and an improper lipid profile may predispose them to atherosclerosis in the long run. This data suggests the need of further studies to determine the need for metabolic cardiovascular risk screening in children with CD.

ACS Style

Anna Rybak; Aldona Wierzbicka; Piotr Socha; Anna Stolarczyk; Bożena Cukrowska; Łukasz Obrycki; Zbigniew Wawer; Roman Janas; Beata Oralewska; Anna Szaflarska-Popławska; Barbara Iwańczak; Elżbieta Cyrta-Jarocka; Urszula Grzybowska-Chlebowczyk; Wojciech Cichy; Grażyna Czaja-Bulsa; Jerzy Socha. Atherosclerotic Risk Factors in Children with Celiac Disease. Gastroenterology Research and Practice 2020, 2020, 1 -9.

AMA Style

Anna Rybak, Aldona Wierzbicka, Piotr Socha, Anna Stolarczyk, Bożena Cukrowska, Łukasz Obrycki, Zbigniew Wawer, Roman Janas, Beata Oralewska, Anna Szaflarska-Popławska, Barbara Iwańczak, Elżbieta Cyrta-Jarocka, Urszula Grzybowska-Chlebowczyk, Wojciech Cichy, Grażyna Czaja-Bulsa, Jerzy Socha. Atherosclerotic Risk Factors in Children with Celiac Disease. Gastroenterology Research and Practice. 2020; 2020 ():1-9.

Chicago/Turabian Style

Anna Rybak; Aldona Wierzbicka; Piotr Socha; Anna Stolarczyk; Bożena Cukrowska; Łukasz Obrycki; Zbigniew Wawer; Roman Janas; Beata Oralewska; Anna Szaflarska-Popławska; Barbara Iwańczak; Elżbieta Cyrta-Jarocka; Urszula Grzybowska-Chlebowczyk; Wojciech Cichy; Grażyna Czaja-Bulsa; Jerzy Socha. 2020. "Atherosclerotic Risk Factors in Children with Celiac Disease." Gastroenterology Research and Practice 2020, no. : 1-9.

Journal article
Published: 24 February 2020 in Cells
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Disruption of epithelial junctional complex (EJC), especially tight junctions (TJ), resulting in increased intestinal permeability, is supposed to activate the enhanced immune response to gluten and to induce the development of celiac disease (CD). This study is aimed to present the role of EJC in CD pathogenesis. To analyze differentially expressed genes the next-generation mRNA sequencing data from CD326+ epithelial cells isolated from non-celiac and celiac patients were involved. Ultrastructural studies with morphometry of EJC were done in potential CD, newly recognized active CD, and non-celiac controls. The transcriptional analysis suggested disturbances of epithelium and the most significant gene ontology enriched terms in epithelial cells from CD patients related to the plasma membrane, extracellular exome, extracellular region, and extracellular space. Ultrastructural analyses showed significantly tighter TJ, anomalies in desmosomes, dilatations of intercellular space, and shorter microvilli in potential and active CD compared to controls. Enterocytes of fetal-like type and significantly wider adherence junctions were observed only in active CD. In conclusion, the results do not support the hypothesis that an increased passage of gluten peptides by unsealing TJ precedes CD development. However, increased intestinal permeability due to abnormality of epithelium might play a role in CD onset.

ACS Style

Agnieszka Sowińska; Yasser Morsy; Elżbieta Czarnowska; Beata Oralewska; Ewa Konopka; Marek Woynarowski; Sylwia Szymańska; Maria Ejmont; Michael Scharl; Joanna B. Bierła; Marcin Wawrzyniak; Bożena Cukrowska. Transcriptional and Ultrastructural Analyses Suggest Novel Insights into Epithelial Barrier Impairment in Celiac Disease. Cells 2020, 9, 516 .

AMA Style

Agnieszka Sowińska, Yasser Morsy, Elżbieta Czarnowska, Beata Oralewska, Ewa Konopka, Marek Woynarowski, Sylwia Szymańska, Maria Ejmont, Michael Scharl, Joanna B. Bierła, Marcin Wawrzyniak, Bożena Cukrowska. Transcriptional and Ultrastructural Analyses Suggest Novel Insights into Epithelial Barrier Impairment in Celiac Disease. Cells. 2020; 9 (2):516.

Chicago/Turabian Style

Agnieszka Sowińska; Yasser Morsy; Elżbieta Czarnowska; Beata Oralewska; Ewa Konopka; Marek Woynarowski; Sylwia Szymańska; Maria Ejmont; Michael Scharl; Joanna B. Bierła; Marcin Wawrzyniak; Bożena Cukrowska. 2020. "Transcriptional and Ultrastructural Analyses Suggest Novel Insights into Epithelial Barrier Impairment in Celiac Disease." Cells 9, no. 2: 516.

Journal article
Published: 13 February 2019 in Toxins
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The aim of the study was to investigate the toxic effects of aflatoxin B1 (AFB1) and efficacy of a probiotic preparation containing L. reuteri, L. plantarum, L. pentosus, L. rhamnosus and L. paracasei and Saccharomyces cerevisiae yeasts to ameliorate their effects in broiler chickens. A total of 168 one-day-old female Ross 308 broilers were randomly allocated to six groups. Three wheat and soybean meal-based diets were prepared: Control diet and diets contaminated with 1 or 5 mg/kg AFB1 supplied in moldy wheat. All diets were unsupplemented or supplemented with probiotic, cold pelleted and fed from 1 to 35 day of life. Feeding diet with 1 mg AFB1/kg did not affect performance, but a diet with 5 mg AFB1 resulted in a significant reduction of feed intake and BWG, both diets induced liver and kidneys enlargement. The probiotic supplementation of the diets partially ameliorated those negative effects and resulted in a significant increase of AFB1 excretion. It was accompanied by the reduced level of AFB1 residues in the liver from 8.9 to 3.7 and from 11.8 to 5.9 µg/kg, in kidneys from 7.9 to 2.5 and from 13.7 to 4.1 µg/kg in birds fed the less and more contaminated diets, respectively. AFB1 exposure caused many severe histopathological changes in the liver and kidneys of broilers, probiotic supplementation significantly reduced the changes of these organs. It may be concluded that the probiotic supplementation can be used to alleviate the negative effects of contamination of broiler feed with AFB1 on bird health and product security.

ACS Style

Katarzyna Śliżewska; Bożena Cukrowska; Stefania Smulikowska; Joanna Cielecka-Kuszyk. The Effect of Probiotic Supplementation on Performance and the Histopathological Changes in Liver and Kidneys in Broiler Chickens Fed Diets with Aflatoxin B1. Toxins 2019, 11, 112 .

AMA Style

Katarzyna Śliżewska, Bożena Cukrowska, Stefania Smulikowska, Joanna Cielecka-Kuszyk. The Effect of Probiotic Supplementation on Performance and the Histopathological Changes in Liver and Kidneys in Broiler Chickens Fed Diets with Aflatoxin B1. Toxins. 2019; 11 (2):112.

Chicago/Turabian Style

Katarzyna Śliżewska; Bożena Cukrowska; Stefania Smulikowska; Joanna Cielecka-Kuszyk. 2019. "The Effect of Probiotic Supplementation on Performance and the Histopathological Changes in Liver and Kidneys in Broiler Chickens Fed Diets with Aflatoxin B1." Toxins 11, no. 2: 112.

Review
Published: 18 October 2018 in Nutrients
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The “microbiota hypothesis” ties the increase in allergy rates observed in highly developed countries over the last decades to disturbances in the gut microbiota. Gut microbiota formation depends on a number of factors and occurs over approximately 1000 days of life, including the prenatal period. During this period the microbiota helps establish the functional immune phenotype, including immune tolerance. The development of immune tolerance depends also on early exposure to potential food allergens, a process referred to as nutritional programming. This article elaborates on the concepts of microbial and nutritional programming and their role in the primary prevention of allergy.

ACS Style

Bożena Cukrowska. Microbial and Nutritional Programming—The Importance of the Microbiome and Early Exposure to Potential Food Allergens in the Development of Allergies. Nutrients 2018, 10, 1541 .

AMA Style

Bożena Cukrowska. Microbial and Nutritional Programming—The Importance of the Microbiome and Early Exposure to Potential Food Allergens in the Development of Allergies. Nutrients. 2018; 10 (10):1541.

Chicago/Turabian Style

Bożena Cukrowska. 2018. "Microbial and Nutritional Programming—The Importance of the Microbiome and Early Exposure to Potential Food Allergens in the Development of Allergies." Nutrients 10, no. 10: 1541.

Journal article
Published: 01 October 2018 in Postępy Higieny i Medycyny Doświadczalnej
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Introduction: Hypersensitivity to ionising radiation is most often observed in the course of primary immunodeficiency diseases, which are associated with dysfunctional DNA repair, especially with the repair of double-strand breaks. Due to phenotypic similarities between primary immunodeficiency diseases, radiosensitivity testing can prove useful in early differential diagnosis, when attempting to identify patients with increased toxic reactivity to radio- and chemotherapy, and can have an impact on the process of their preparation for stem cell transplantation. Aim: The aim of the study was to assess the radiosensitivity in vitro of patients with ataxia-telangiectasia (A-T) syndrome, and their parents, carriers of one copy of the mutated ATM gene. Material/Methods: Lymphoblastoid cell lines (LCLs) from 15 A-T patients (remaining under the care of the Immunology Clinic and Immunology Outpatient Clinic of the Children’s Memorial Health Institute) and 11 mothers and 11 fathers of A-T patients, were used for radiosensitivity assessment. A standard colony survival assay (CSA) was applied in the tests. Results: A markedly decreased survival fraction (SF) of LCLs after in vitro exposure to X-rays was observed in all A-T patients when compared to control cells. A clear diversification of radiosensitivity to ionising radiation was observed among obligate heterozygotes. SF for heterozygotes was between 1% and 53%, i.e. varied from the values in healthy individuals to the extreme values observed in A-T patients. Conclusion: The assessment of cell radiosensitivity in A-T patients using CSA may be a useful additional test for confirming a clinically suspected disease. In heterozygous carriers, it can be an indicator of increased risk of carcinogenesis, and in both A-T patients and their parents can be helpful in making decisions with regard to radio- and/or chemotherapy.

ACS Style

Barbara Pietrucha; Hanna Gregorek; Edyta Heropolitańska-Pliszka; Bożena Cukrowska; Ewa Konopka; Ewa Bernatowska. The assessment of radiosensitivity in patients with ataxia-telangiectasia syndrome and in carriers of the mutated ATM gene using lymphoblastoid cell lines. Postępy Higieny i Medycyny Doświadczalnej 2018, 72, 846 -852.

AMA Style

Barbara Pietrucha, Hanna Gregorek, Edyta Heropolitańska-Pliszka, Bożena Cukrowska, Ewa Konopka, Ewa Bernatowska. The assessment of radiosensitivity in patients with ataxia-telangiectasia syndrome and in carriers of the mutated ATM gene using lymphoblastoid cell lines. Postępy Higieny i Medycyny Doświadczalnej. 2018; 72 ():846-852.

Chicago/Turabian Style

Barbara Pietrucha; Hanna Gregorek; Edyta Heropolitańska-Pliszka; Bożena Cukrowska; Ewa Konopka; Ewa Bernatowska. 2018. "The assessment of radiosensitivity in patients with ataxia-telangiectasia syndrome and in carriers of the mutated ATM gene using lymphoblastoid cell lines." Postępy Higieny i Medycyny Doświadczalnej 72, no. : 846-852.

Research article
Published: 11 June 2018 in Mediators of Inflammation
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Oxidative stress (OS) has been recently implicated in the disease pathogenesis in inflammatory bowel disease (IBD). The aim of the study was to evaluate oxidative and antioxidative stress status and the risk of the atherosclerotic process in children with IBD and functional gastrointestinal disorders (FGID). The prospective study included a group of 71 children during a period of 2 years. In all children, laboratory tests were performed and intima-media complex in the carotid artery was measured (IMC). Low values of OS were more frequent in children with IBD than in the FGID group. The average concentration of oxidized lipoprotein with average density (oxLDL) was lower in patients with IBD. Among patients with IBD, higher concentrations of oxLDL were recorded in patients with longer-duration disease and with higher concentrations of total cholesterol. In the IBD group, more often, higher concentrations of anti-oxLDL were recorded among patients with longer-duration disease. The obtained results did not support the hypothesis of total antioxidant capacity depletion and greater overall OS in patients with IBD. Patients with IBD with a longer duration of the disease have higher concentrations of oxLDL and anti-oxLDL.

ACS Style

Urszula Grzybowska-Chlebowczyk; Paulina Wysocka-Wojakiewicz; Martyna Jasielska; Bożena Cukrowska; Sabina Więcek; Maria Kniażewska; Jerzy Chudek. Oxidative and Antioxidative Stress Status in Children with Inflammatory Bowel Disease as a Result of a Chronic Inflammatory Process. Mediators of Inflammation 2018, 2018, 1 -7.

AMA Style

Urszula Grzybowska-Chlebowczyk, Paulina Wysocka-Wojakiewicz, Martyna Jasielska, Bożena Cukrowska, Sabina Więcek, Maria Kniażewska, Jerzy Chudek. Oxidative and Antioxidative Stress Status in Children with Inflammatory Bowel Disease as a Result of a Chronic Inflammatory Process. Mediators of Inflammation. 2018; 2018 ():1-7.

Chicago/Turabian Style

Urszula Grzybowska-Chlebowczyk; Paulina Wysocka-Wojakiewicz; Martyna Jasielska; Bożena Cukrowska; Sabina Więcek; Maria Kniażewska; Jerzy Chudek. 2018. "Oxidative and Antioxidative Stress Status in Children with Inflammatory Bowel Disease as a Result of a Chronic Inflammatory Process." Mediators of Inflammation 2018, no. : 1-7.

Laboratory investigation
Published: 09 February 2018 in Journal of Neuro-Oncology
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Medulloblastoma, the most common malignant pediatric brain tumor, is a heterogeneous disease, with the existence of at least four molecular types: Wingless (WNT), Sonic Hedgehog (SHH), Group 3 and Group 4 tumors. The latter two groups, which can be identified by an application of multi-gene expression or methylation profiling, show sometimes ambiguous categorization and are still classified for diagnostic reason as non-SHH/non-WNT medulloblastomas in updated WHO 2016 classification. In order to better characterize non-SHH/non-WNT tumors, we applied the method based on the Nanostring nCounter Technology, using the 26 genes codeset in 68 uniformly treated medulloblastoma patients. This allowed for identification of tumors, which shared common Group 3 and Group 4 gene signatures. We recognized three transcriptional groups within non-WNT/non-SHH tumors: Group 3, Group 4 and the Intermediate 3/4 Group. Group 3, in line with previously published results, showed poor prognosis with survival rate < 40%, frequent metastases, large cell/anaplastic pathology and presence of tumors with MYCC amplification. This is in contrast to patients from the Intermediate 3/4 Group who showed the best survival rate (100%). Overall and progression free survival were better for this group than for Group 3 (p = 0.001, for both) and Group 4 (p = 0.064 and p = 0.066, respectively). Our work supports the view that within the non-WNT/non-SHH tumors different risk groups exist and that the current two groups classifier may be not sufficient for proper clinical categorization of individual patients.

ACS Style

Maria Łastowska; Joanna Trubicka; Magdalena Niemira; Magdalena Paczkowska-Abdulsalam; Agnieszka Karkucińska-Więckowska; Magdalena Kaleta; Monika Drogosiewicz; Marta Perek-Polnik; Adam Kretowski; Bożena Cukrowska; Wiesława Grajkowska; Bozenna Dembowska-Bagińska; Ewa Matyja. Medulloblastoma with transitional features between Group 3 and Group 4 is associated with good prognosis. Journal of Neuro-Oncology 2018, 138, 231 -240.

AMA Style

Maria Łastowska, Joanna Trubicka, Magdalena Niemira, Magdalena Paczkowska-Abdulsalam, Agnieszka Karkucińska-Więckowska, Magdalena Kaleta, Monika Drogosiewicz, Marta Perek-Polnik, Adam Kretowski, Bożena Cukrowska, Wiesława Grajkowska, Bozenna Dembowska-Bagińska, Ewa Matyja. Medulloblastoma with transitional features between Group 3 and Group 4 is associated with good prognosis. Journal of Neuro-Oncology. 2018; 138 (2):231-240.

Chicago/Turabian Style

Maria Łastowska; Joanna Trubicka; Magdalena Niemira; Magdalena Paczkowska-Abdulsalam; Agnieszka Karkucińska-Więckowska; Magdalena Kaleta; Monika Drogosiewicz; Marta Perek-Polnik; Adam Kretowski; Bożena Cukrowska; Wiesława Grajkowska; Bozenna Dembowska-Bagińska; Ewa Matyja. 2018. "Medulloblastoma with transitional features between Group 3 and Group 4 is associated with good prognosis." Journal of Neuro-Oncology 138, no. 2: 231-240.

Journal article
Published: 01 February 2018 in Journal of Medicinal Food
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There is evidence that children with autism spectrum disorders (ASDs) display an increased immune reactivity against gluten, which is supposed to be the effect of intestinal barrier abnormalities. The aim of study was to evaluate the relation of antibody induced by gluten to zonulin and intestinal fatty acid binding proteins (I-FABP), that is, serological markers of an impaired gut barrier. The study included 77 patients with ASDs. Zonulin, I-FABP, celiac-specific antibodies, anti-gliadin antibodies (AGA), and antibodies against neural transglutaminase 6 (TG6) of immunoglobulin (Ig) A and IgG classes were detected in sera. Celiac-specific antibodies were negative in all ASD children, four children (5.2%) had positive anti-TG6 antibodies, and increased AGA-IgG production was found in 21 patients (27.3%). Mean levels of zonulin and I-FABP in ASD patients were similar to those found in healthy controls and revealed a negative correlation with age, whereas regression analysis revealed a significant positive relationship between antibody production and the age. Serum concentrations of zonulin and I-FABP showed no statistically significant association with antibody positivity. An increased production of antibodies related to gliadin and neural TG6 in ASD children is not related to serological markers of an impaired intestinal barrier.

ACS Style

Jan Józefczuk; Ewa Konopka; Joanna Bierla; Ilona Trojanowska; Agnieszka Sowińska; Rafał Czarnecki; Lucjan Sobol; Paweł Józefczuk; Weronika Surdy; Bozena Cukrowska. The Occurrence of Antibodies Against Gluten in Children with Autism Spectrum Disorders Does Not Correlate with Serological Markers of Impaired Intestinal Permeability. Journal of Medicinal Food 2018, 21, 181 -187.

AMA Style

Jan Józefczuk, Ewa Konopka, Joanna Bierla, Ilona Trojanowska, Agnieszka Sowińska, Rafał Czarnecki, Lucjan Sobol, Paweł Józefczuk, Weronika Surdy, Bozena Cukrowska. The Occurrence of Antibodies Against Gluten in Children with Autism Spectrum Disorders Does Not Correlate with Serological Markers of Impaired Intestinal Permeability. Journal of Medicinal Food. 2018; 21 (2):181-187.

Chicago/Turabian Style

Jan Józefczuk; Ewa Konopka; Joanna Bierla; Ilona Trojanowska; Agnieszka Sowińska; Rafał Czarnecki; Lucjan Sobol; Paweł Józefczuk; Weronika Surdy; Bozena Cukrowska. 2018. "The Occurrence of Antibodies Against Gluten in Children with Autism Spectrum Disorders Does Not Correlate with Serological Markers of Impaired Intestinal Permeability." Journal of Medicinal Food 21, no. 2: 181-187.

Review
Published: 14 November 2017 in World Journal of Gastroenterology
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Celiac disease (CD) is a chronic immune-mediated disorder triggered by the ingestion of gluten in genetically predisposed individuals. Before activating the immune system, gluten peptides are transferred by the epithelial barrier to the mucosal lamina propria, where they are deamidated by intestinal tissue transglutaminase 2. As a result, they strongly bind to human leucocyte antigens (HLAs), especially HLA-DQ2 and HLA-DQ8, expressed on antigen-presenting cells. This induces an inflammatory response, which results in small bowel enteropathy. Although gluten is the main external trigger activating both innate and adaptive (specific) immunity, its presence in the intestinal lumen does not fully explain CD pathogenesis. It has been hypothesized that an early disruption of the gut barrier in genetically susceptible individuals, which would result in an increased intestinal permeability, could precede the onset of gluten-induced immune events. The intestinal barrier is a complex functional structure, whose functioning is dependent on intestinal microbiota homeostasis, epithelial layer integrity, and the gut-associated lymphoid tissue with its intraepithelial lymphocytes (IELs). The aim of this paper was to review the current literature and summarize the role of the gut microbiota, epithelial cells and their intercellular junctions, and IELs in CD development.

ACS Style

Bożena Cukrowska; Agnieszka Sowińska; Joanna Beata Bierła; Elżbieta Czarnowska; Anna Rybak; Urszula Grzybowska-Chlebowczyk. Intestinal epithelium, intraepithelial lymphocytes and the gut microbiota - Key players in the pathogenesis of celiac disease. World Journal of Gastroenterology 2017, 23, 7505 -7518.

AMA Style

Bożena Cukrowska, Agnieszka Sowińska, Joanna Beata Bierła, Elżbieta Czarnowska, Anna Rybak, Urszula Grzybowska-Chlebowczyk. Intestinal epithelium, intraepithelial lymphocytes and the gut microbiota - Key players in the pathogenesis of celiac disease. World Journal of Gastroenterology. 2017; 23 (42):7505-7518.

Chicago/Turabian Style

Bożena Cukrowska; Agnieszka Sowińska; Joanna Beata Bierła; Elżbieta Czarnowska; Anna Rybak; Urszula Grzybowska-Chlebowczyk. 2017. "Intestinal epithelium, intraepithelial lymphocytes and the gut microbiota - Key players in the pathogenesis of celiac disease." World Journal of Gastroenterology 23, no. 42: 7505-7518.

Multicenter study
Published: 01 October 2017 in Gastroenterology
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The guidelines of the European Society of Pediatric Gastroenterology, Hepatology, and Nutrition allow for diagnosis of celiac disease without biopsies in children with symptoms and levels of immunoglobulin A against tissue-transglutaminase (TGA-IgA) 10-fold or more the upper limit of normal (ULN), confirmed by detection of endomysium antibodies (EMA) and positivity for HLA-DQ2/DQ8. We performed a large, international prospective study to validate this approach. We collected data from consecutive pediatric patients (18 years or younger) on a gluten-containing diet who tested positive for TGA-IgA from November 2011 through May 2014, seen at 33 pediatric gastroenterology units in 21 countries. Local centers recorded symptoms; measurements of total IgA, TGA, and EMA; and histopathology findings from duodenal biopsies. Children were considered to have malabsorption if they had chronic diarrhea, weight loss (or insufficient gain), growth failure, or anemia. We directly compared central findings from 16 antibody tests (8 for TGA-IgA, 1 for TGA-IgG, 6 for IgG against deamidated gliadin peptides, and 1 for EMA, from 5 different manufacturers), 2 HLA-DQ2/DQ8 tests from 2 manufacturers, and histopathology findings from the reference pathologist. Final diagnoses were based on local and central results. If all local and central results were concordant for celiac disease, cases were classified as proven celiac disease. Patients with only a low level of TGA-IgA (threefold or less the ULN) but no other results indicating celiac disease were classified as no celiac disease. Central histo-morphometry analyses were performed on all other biopsies and cases were carefully reviewed in a blinded manner. Inconclusive cases were regarded as not having celiac disease for calculation of diagnostic accuracy. The primary aim was to determine whether the nonbiopsy approach identifies children with celiac disease with a positive predictive value (PPV) above 99% in clinical practice. Secondary aims included comparing performance of different serological tests and to determine whether the suggested criteria can be simplified. Of 803 children recruited for the study, 96 were excluded due to incomplete data, low level of IgA, or poor-quality biopsies. In the remaining 707 children (65.1% girls; median age, 6.2 years), 645 were diagnosed with celiac disease, 46 were found not to have celiac disease, and 16 had inconclusive results. Findings from local laboratories of TGA-IgA 10-fold or more the ULN, a positive result from the test for EMA, and any symptom identified children with celiac disease (n = 399) with a PPV of 99.75 (95% confidence interval [CI], 98.61–99.99); the PPV was 100.00 (95% CI, 98.68–100.00) when only malabsorption symptoms were used instead of any symptom (n = 278). Inclusion of HLA analyses did not increase accuracy. Findings from central laboratories differed greatly for patients with lower levels of antibodies, but when levels of TGA-IgA were 10-fold or more the ULN, PPVs ranged from 99.63 (95% CI, 98.67–99.96) to 100.00 (95% CI, 99.23–100.00). Children can be accurately diagnosed with celiac disease without biopsy analysis. Diagnosis based on level of TGA-IgA 10-fold or more the ULN, a positive result from the EMA tests in a second blood sample, and the presence of at least 1 symptom could avoid risks and costs of endoscopy for more than half the children with celiac disease worldwide. HLA analysis is not required for accurate diagnosis. Clinical Trial Registration no: DRKS00003555.

ACS Style

Katharina Julia Werkstetter; Ilma Rita Korponay-Szabó; Alina Popp; Vincenzo Villanacci; Marianna Salemme; Gabriele Heilig; Søren Thue Lillevang; Maria Luisa Mearin; Carmen Ribes-Koninckx; Adrian Thomas; Riccardo Troncone; Birgit Filipiak; Markku Mäki; Judit Gyimesi; Mehri Najafi; Jernej Dolinšek; Stine Dydensborg Sander; Renata Auricchio; Alexandra Papadopoulou; Andreas Vécsei; Peter Szitanyi; Ester Donat; Rafaella Nenna; Philippe Alliet; Francesca Penagini; Hélène Garnier-Lengliné; Gemma Castillejo; Kalle Kurppa; Raanan Shamir; Almuthe Christine Hauer; Françoise Smets; Susana Corujeira; Myriam van Winckel; Stefan Buderus; Sonny Chong; Steffen Husby; Sibylle Koletzko; Piotr Socha; Bozena Cukrowska; Hania Szajewska; Jan Wyhowski; Nailah Brown; Gauri Batra; Zrinjka Misak; Sven Seiwerth; Yulia Dmitrieva; Dmitry Abramov; Yvan Vandenplas; Annieta Goossens; Maaike W. Schaart; V.T.H.B.M. Smit; Nicolas Kalach; Pierre Gosset; Judit B. Kovács; Anikó Nagy; Ilona Lellei; Rita Kőbányai; Katayoun Khatami; Maryam Monajemzadeh; Konstantina Dimakou; Amalia Patereli; Tine Plato Hansen; Rajko Kavalar; Miguel Bolonio; David Ramos; Hubert Kogler; Gabriele Amann; Roberta Kosova; Mariantonia Maglio; Elke Janssens; Ruth Achten; Pavel Frűhauf; Helena Skálová; Thomas Kirchner; Laura Petrarca; Fabio Massimo Magliocca; Francesc Martínez; Vanesa Morente; Sonja Thanner-Lechner; Manfred Ratschek; Marco Gasparetto; Liz Hook; Danielle Canioni; Catherine Wanty; Anne Mourin; Kaija Laurila; Martine Vornane; Vered Nachmias Friedler; Sara L. Morgenstern; Jorge Amil Dias; Fátima Carneiro; Hospital S. João; Stephanie Van Biervliet; Saskia Vande Velde; Hany Banoub; Steve Sampson; Annette M. Müller; Adina Ene; Mandana Rafeey; Amir Taher Eftekhar Sadat. Accuracy in Diagnosis of Celiac Disease Without Biopsies in Clinical Practice. Gastroenterology 2017, 153, 924 -935.

AMA Style

Katharina Julia Werkstetter, Ilma Rita Korponay-Szabó, Alina Popp, Vincenzo Villanacci, Marianna Salemme, Gabriele Heilig, Søren Thue Lillevang, Maria Luisa Mearin, Carmen Ribes-Koninckx, Adrian Thomas, Riccardo Troncone, Birgit Filipiak, Markku Mäki, Judit Gyimesi, Mehri Najafi, Jernej Dolinšek, Stine Dydensborg Sander, Renata Auricchio, Alexandra Papadopoulou, Andreas Vécsei, Peter Szitanyi, Ester Donat, Rafaella Nenna, Philippe Alliet, Francesca Penagini, Hélène Garnier-Lengliné, Gemma Castillejo, Kalle Kurppa, Raanan Shamir, Almuthe Christine Hauer, Françoise Smets, Susana Corujeira, Myriam van Winckel, Stefan Buderus, Sonny Chong, Steffen Husby, Sibylle Koletzko, Piotr Socha, Bozena Cukrowska, Hania Szajewska, Jan Wyhowski, Nailah Brown, Gauri Batra, Zrinjka Misak, Sven Seiwerth, Yulia Dmitrieva, Dmitry Abramov, Yvan Vandenplas, Annieta Goossens, Maaike W. Schaart, V.T.H.B.M. Smit, Nicolas Kalach, Pierre Gosset, Judit B. Kovács, Anikó Nagy, Ilona Lellei, Rita Kőbányai, Katayoun Khatami, Maryam Monajemzadeh, Konstantina Dimakou, Amalia Patereli, Tine Plato Hansen, Rajko Kavalar, Miguel Bolonio, David Ramos, Hubert Kogler, Gabriele Amann, Roberta Kosova, Mariantonia Maglio, Elke Janssens, Ruth Achten, Pavel Frűhauf, Helena Skálová, Thomas Kirchner, Laura Petrarca, Fabio Massimo Magliocca, Francesc Martínez, Vanesa Morente, Sonja Thanner-Lechner, Manfred Ratschek, Marco Gasparetto, Liz Hook, Danielle Canioni, Catherine Wanty, Anne Mourin, Kaija Laurila, Martine Vornane, Vered Nachmias Friedler, Sara L. Morgenstern, Jorge Amil Dias, Fátima Carneiro, Hospital S. João, Stephanie Van Biervliet, Saskia Vande Velde, Hany Banoub, Steve Sampson, Annette M. Müller, Adina Ene, Mandana Rafeey, Amir Taher Eftekhar Sadat. Accuracy in Diagnosis of Celiac Disease Without Biopsies in Clinical Practice. Gastroenterology. 2017; 153 (4):924-935.

Chicago/Turabian Style

Katharina Julia Werkstetter; Ilma Rita Korponay-Szabó; Alina Popp; Vincenzo Villanacci; Marianna Salemme; Gabriele Heilig; Søren Thue Lillevang; Maria Luisa Mearin; Carmen Ribes-Koninckx; Adrian Thomas; Riccardo Troncone; Birgit Filipiak; Markku Mäki; Judit Gyimesi; Mehri Najafi; Jernej Dolinšek; Stine Dydensborg Sander; Renata Auricchio; Alexandra Papadopoulou; Andreas Vécsei; Peter Szitanyi; Ester Donat; Rafaella Nenna; Philippe Alliet; Francesca Penagini; Hélène Garnier-Lengliné; Gemma Castillejo; Kalle Kurppa; Raanan Shamir; Almuthe Christine Hauer; Françoise Smets; Susana Corujeira; Myriam van Winckel; Stefan Buderus; Sonny Chong; Steffen Husby; Sibylle Koletzko; Piotr Socha; Bozena Cukrowska; Hania Szajewska; Jan Wyhowski; Nailah Brown; Gauri Batra; Zrinjka Misak; Sven Seiwerth; Yulia Dmitrieva; Dmitry Abramov; Yvan Vandenplas; Annieta Goossens; Maaike W. Schaart; V.T.H.B.M. Smit; Nicolas Kalach; Pierre Gosset; Judit B. Kovács; Anikó Nagy; Ilona Lellei; Rita Kőbányai; Katayoun Khatami; Maryam Monajemzadeh; Konstantina Dimakou; Amalia Patereli; Tine Plato Hansen; Rajko Kavalar; Miguel Bolonio; David Ramos; Hubert Kogler; Gabriele Amann; Roberta Kosova; Mariantonia Maglio; Elke Janssens; Ruth Achten; Pavel Frűhauf; Helena Skálová; Thomas Kirchner; Laura Petrarca; Fabio Massimo Magliocca; Francesc Martínez; Vanesa Morente; Sonja Thanner-Lechner; Manfred Ratschek; Marco Gasparetto; Liz Hook; Danielle Canioni; Catherine Wanty; Anne Mourin; Kaija Laurila; Martine Vornane; Vered Nachmias Friedler; Sara L. Morgenstern; Jorge Amil Dias; Fátima Carneiro; Hospital S. João; Stephanie Van Biervliet; Saskia Vande Velde; Hany Banoub; Steve Sampson; Annette M. Müller; Adina Ene; Mandana Rafeey; Amir Taher Eftekhar Sadat. 2017. "Accuracy in Diagnosis of Celiac Disease Without Biopsies in Clinical Practice." Gastroenterology 153, no. 4: 924-935.

Article
Published: 01 June 2017 in Journal of Pediatric Gastroenterology & Nutrition
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It is unclear whether a distinct activity of pathways removing the antitrypsin (AT) protein in Alpha-1-Antitrypsin Deficiency (α1ATD) are associated with an unfavorable predisposition to liver disease in the future. The aim of this study was to determine whether liverspecific activity of AT protein disposal occurs at infancy in α1ATD with PiZZ phenotype (ATZ). Liver samples of 17 infants with unfavorable ATZ outcome (Group I, n = 8, median age = 0.35 year) and good outcome (Group II, n = 9, 0.17 year), and 9 with biliary atresia (BA, median age = 0.17 year) as control, were enrolled. For each subject were investigated autophagy activity by mRNA, protein expression (Calnexin, Beclin-1, p62, and Parkin), and hepatocyte ultrastructure with morphometric analyses. No significant differences in gene expression in the liver of infants were found between the 2 ATZ groups. Although a correlation between patients’ age and protein expression was observed, the ATZ groups differed Parkin immunohistochemical expression. Moreover, the hepatocytes in ATZ infants with unfavorable outcome were characterized by low Parkin expression and the presence of isolated mitophagosoms and numerous enlarged mitochondria. The mentioned findings differed in patients with BA. Thus, mentioned specific features occurring at infancy may suggest association with poor liver outcome. Parkin low expression could have a potential for disease prognosis and treatment; however, further studies in a greater number of patients are needed.

ACS Style

Elzbieta Czarnowska; Agnieszka Bakula; Joanna Bierla; Justyna Niderla-Bielińska; Agnieszka Sowińska; Bozena Cukrowska; Joanna Cielecka-Kuszyk; Piotr Socha. Autophagy in Hepatocytes in Infants With Alpha-1 ATD and Different Liver Disease Outcomes: A Retrospective Analysis. Journal of Pediatric Gastroenterology & Nutrition 2017, 64, 876 -882.

AMA Style

Elzbieta Czarnowska, Agnieszka Bakula, Joanna Bierla, Justyna Niderla-Bielińska, Agnieszka Sowińska, Bozena Cukrowska, Joanna Cielecka-Kuszyk, Piotr Socha. Autophagy in Hepatocytes in Infants With Alpha-1 ATD and Different Liver Disease Outcomes: A Retrospective Analysis. Journal of Pediatric Gastroenterology & Nutrition. 2017; 64 (6):876-882.

Chicago/Turabian Style

Elzbieta Czarnowska; Agnieszka Bakula; Joanna Bierla; Justyna Niderla-Bielińska; Agnieszka Sowińska; Bozena Cukrowska; Joanna Cielecka-Kuszyk; Piotr Socha. 2017. "Autophagy in Hepatocytes in Infants With Alpha-1 ATD and Different Liver Disease Outcomes: A Retrospective Analysis." Journal of Pediatric Gastroenterology & Nutrition 64, no. 6: 876-882.

Comparative study
Published: 26 February 2016 in PLOS ONE
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Recent advances in culture-independent approaches have enabled insights into the diversity, complexity, and individual variability of gut microbial communities. To examine the effect of oral administration of Saccharomyces (S.) boulardii and mode of delivery on the intestinal microbial community in preterm infants. Stool samples were collected from preterm newborns randomly divided into two groups: a probiotic-receiving group (n = 18) or a placebo group (n = 21). Samples were collected before probiotic intake (day 0), and after 2 and 6 weeks of supplementation. The composition of colonizing bacteria was assessed by 16S ribosomal RNA (rRNA) gene sequencing of fecal samples using the Ion 16S Metagenomics Kit and the Ion Torrent Personal Genome Machine platform. A total of 11932257 reads were generated, and were clustered into 459, 187, and 176 operational taxonomic units at 0 days, 2 weeks, and 6 weeks, respectively. Of the 17 identified phyla, Firmicutes Actinobacteria, Proteobacteria, and Bacteroidetes were universal. The microbial community differed at day 0 compared with at 2 weeks and 6 weeks. There was a tendency for increased bacterial diversity at 2 weeks and 6 weeks compared with day 0, and infants with a gestational age of 31 weeks or higher presented increased bacterial diversity prior to S. boulardii administration. Firmicutes and Proteobacteria remained stable during the observation period, whereas Actinobacteria and Bacteroidetes increased in abundance, the latter particularly more sharply in vaginally delivered infants. While the mode of delivery may influence the development of a microbial community, this study had not enough power to detect statistical differences between cohorts supplemented with probiotics, and in a consequence, to speculate on S. boulardii effect on gut microbiome composition in preterm newborns.

ACS Style

Natalia Żeber-Lubecka; Maria Kulecka; Filip Ambrożkiewicz; Agnieszka Paziewska; Milosz Lechowicz; Ewa Konopka; Urszula Majewska; Maria Borszewska-Kornacka; Michal Mikula; Bozena Cukrowska; Jerzy Ostrowski. Effect of Saccharomyces boulardii and Mode of Delivery on the Early Development of the Gut Microbial Community in Preterm Infants. PLOS ONE 2016, 11, e0150306 .

AMA Style

Natalia Żeber-Lubecka, Maria Kulecka, Filip Ambrożkiewicz, Agnieszka Paziewska, Milosz Lechowicz, Ewa Konopka, Urszula Majewska, Maria Borszewska-Kornacka, Michal Mikula, Bozena Cukrowska, Jerzy Ostrowski. Effect of Saccharomyces boulardii and Mode of Delivery on the Early Development of the Gut Microbial Community in Preterm Infants. PLOS ONE. 2016; 11 (2):e0150306.

Chicago/Turabian Style

Natalia Żeber-Lubecka; Maria Kulecka; Filip Ambrożkiewicz; Agnieszka Paziewska; Milosz Lechowicz; Ewa Konopka; Urszula Majewska; Maria Borszewska-Kornacka; Michal Mikula; Bozena Cukrowska; Jerzy Ostrowski. 2016. "Effect of Saccharomyces boulardii and Mode of Delivery on the Early Development of the Gut Microbial Community in Preterm Infants." PLOS ONE 11, no. 2: e0150306.

Journal article
Published: 01 January 2016 in World Journal of Gastrointestinal Pharmacology and Therapeutics
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AIM: To evaluate the clinical utility of multi-antibody strategies in the diagnosis of coeliac disease (CD), the new quantitative Polycheck immunoassays were analysed.

ACS Style

Ewa Konopka; Maciej Grzywnowicz; Beata Oralewska; Joanna Cielecka-Kuszyk; Ilona Trojanowska; Bożena Cukrowska. Clinical utility of quantitative multi-antibody Polycheck immunoassays in the diagnosis of coeliac disease. World Journal of Gastrointestinal Pharmacology and Therapeutics 2016, 7, 254 -260.

AMA Style

Ewa Konopka, Maciej Grzywnowicz, Beata Oralewska, Joanna Cielecka-Kuszyk, Ilona Trojanowska, Bożena Cukrowska. Clinical utility of quantitative multi-antibody Polycheck immunoassays in the diagnosis of coeliac disease. World Journal of Gastrointestinal Pharmacology and Therapeutics. 2016; 7 (2):254-260.

Chicago/Turabian Style

Ewa Konopka; Maciej Grzywnowicz; Beata Oralewska; Joanna Cielecka-Kuszyk; Ilona Trojanowska; Bożena Cukrowska. 2016. "Clinical utility of quantitative multi-antibody Polycheck immunoassays in the diagnosis of coeliac disease." World Journal of Gastrointestinal Pharmacology and Therapeutics 7, no. 2: 254-260.

Journal article
Published: 01 January 2016 in Clinical Laboratory
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ACS Style

Ewa Konopka; Aldona Ceregra; Elżbieta Maciorkowska; Barbara Surowska; Ilona Trojanowska; Izabela Roszko-Kirpsza; Bozena Cukrowska. Specific IgE Antibodies in Young Children with Atopic Dermatitis - Correlation of Multiple Allergen Simultaneous Immunoblot Test and ImmunoCap System. Clinical Laboratory 2016, 62, 1 .

AMA Style

Ewa Konopka, Aldona Ceregra, Elżbieta Maciorkowska, Barbara Surowska, Ilona Trojanowska, Izabela Roszko-Kirpsza, Bozena Cukrowska. Specific IgE Antibodies in Young Children with Atopic Dermatitis - Correlation of Multiple Allergen Simultaneous Immunoblot Test and ImmunoCap System. Clinical Laboratory. 2016; 62 (05/2016):1.

Chicago/Turabian Style

Ewa Konopka; Aldona Ceregra; Elżbieta Maciorkowska; Barbara Surowska; Ilona Trojanowska; Izabela Roszko-Kirpsza; Bozena Cukrowska. 2016. "Specific IgE Antibodies in Young Children with Atopic Dermatitis - Correlation of Multiple Allergen Simultaneous Immunoblot Test and ImmunoCap System." Clinical Laboratory 62, no. 05/2016: 1.