Wild terrestrial carnivores play a crucial role as reservoir, maintenance, and spillover hosts for a wide parasite variety. They may harbor, shed, and transmit zoonotic parasites and parasites of veterinary importance for domestic hosts. Although wild carnivores are globally distributed and comprise many different species, some living in close proximity to human settlements, only a few studies have investigated parasites of wild terrestrial carnivores using non-specific techniques. Access to samples of wild carnivores may be challenging as some species are protected, and others are secretive, possibly explaining the data paucity. Considering the importance of wild carnivores’ health and ecological role, combined with the lack of specific diagnostic methodologies, this review aims to offer an overview of the diagnostic methods for parasite investigation in wild terrestrial carnivores, providing the precise techniques for collection and analysis of fecal, blood, and tissue samples, the environmental impact on said samples, and the limitations researchers currently face in analyzing samples of wild terrestrial carnivores. In addition, this paper offers some crucial information on how different environmental factors affect parasite detection postmortem and how insects can be used to estimate the time of death with a specific highlight on insect larvae. The paper contains a literature review of available procedures and emphasizes the need for diagnostic method standardization in wild terrestrial carnivores.Graphical Abstract

Background: Borrelia persica causes tick-borne relapsing fever in Israel, the eastern Mediterranean basin, and Asia. Relapsing fever is associated with severe illness and potentially death in humans and animals. Since B. persica infection has rarely been described in wild animals, the aim of this study was to evaluate the prevalence of infection with B. persica in wild carnivores in Israel. Methods: Spleen and blood clot samples from wild carnivores, which underwent necropsy, were tested for the presence of Borrelia DNA by real-time polymerase chain reaction (PCR). PCR products were sequenced, and the spirochete loads were quantified using a specific quantitative PCR (qPCR). Results: A total of 140 samples from 74 wild carnivores were analyzed for the presence of Borrelia DNA. Six out of the 74 (8.1%) animals were found positive for B. persica by PCR and sequencing of the flagellin B gene, of which 4/74 (5.4%) were also positive by PCR for the glycerophosphodiester phosphodiesterase (glpQ) gene. Positive samples were obtained from three European badgers, and one striped hyena, golden jackal, and red fox each. All B. persica-positive animals were young males (P < 0.0001). Quantifiable results were obtained from 3/5 spleen and 4/5 blood samples. The spirochete loads in the blood were significantly higher than those found in the spleen (P = 0.034). Conclusions: The prevalence of B. persica infection found in wild carnivores brought for necropsy was unexpectedly high, suggesting that this infection is widespread in some wild animal species in Israel. This is the first report of B. persica infection in the European badger and striped hyena. These carnivores have a wide geographical range of activity, and the results of this survey raise the possibility that they may serve as reservoir hosts for B. persica. Graphical

A 4-y-old male Labrador Retriever was submitted for autopsy following radiographic examination by the owner, a veterinarian, which had revealed 3 embedded projectiles. Autopsy revealed a single entrance wound on the left flank. Using flap-by-flap dissection, the trajectory of the projectile was traced through the skin and lumbar musculature to its location where it had perforated the abdominal aorta, causing fatal acute hemoperitoneum. Remarkably, the copper-coated pellet (Diabolo) was found in the lumen of the right femoral artery, consistent with projectile embolism. No external trauma was present at that site. Embolization can occur when a projectile enters the vasculature and travels to a distal location, typically requiring low residual kinetic energy and a vessel of sufficient caliber. Although well documented in human forensic medicine, projectile embolism is exceedingly rare in veterinary cases. To our knowledge, embolization of a projectile in the femoral artery in a dog has not been reported previously. Our case highlights the importance of comprehensive radiographic imaging before autopsy and illustrates the diagnostic value of correlating radiologic findings with meticulous gross examination in veterinary forensic pathology cases.

Brucella canis is a recognized zoonotic pathogen associated with human infection in a variety of epidemiological settings; however, the magnitude of its impact on human health remains uncertain because of its nonspecific clinical presentation, limited availability of species-specific diagnostic methods, and insufficient awareness among physicians, veterinarians, microbiologists, and public health professionals. Unlike classical human brucellosis caused by smooth Brucella species, B. canis infection may be overlooked, potentially leading to delayed diagnosis and incomplete case detection. This narrative review summarizes current evidence on the epidemiology, transmission pathways, occupational and non-occupational risk factors, clinical manifestations, diagnostic approaches, treatment, and public health implications of human B. canis infection. Particular emphasis is placed on published human cases, laboratory-acquired infections, household clusters, cases reported in different age and immune-status groups, and the biological and epidemiological characteristics of canine infection that facilitate zoonotic transmission. The review further examines the limitations of currently available microbiological, serological, and molecular diagnostic methods, the lack of harmonized surveillance systems, and the consequences of inadequate recognition from a One Health perspective. Collectively, the available evidence confirms the zoonotic potential of B. canis but remains insufficient to define the true burden of human infection. Improving clinical awareness, strengthening collaboration between veterinary and human health sectors, expanding access to standardized diagnostic tools, and implementing integrated One Health surveillance may improve recognition and reduce zoonotic risk.