Existing deep Thermal InfraRed (TIR) trackers only use semantic features to represent the TIR object, which lack the sufficient discriminative capacity for handling distractors. This becomes worse when the feature extraction network is only trained on RGB images. To address this issue, we propose a multi-level similarity model under a Siamese framework for robust TIR object tracking. Specifically, we compute different pattern similarities using the proposed multi-level similarity network. One of them focuses on the global semantic similarity and the other computes the local structural similarity of the TIR object. These two similarities complement each other and hence enhance the discriminative capacity of the network for handling distractors. In addition, we design a simple while effective relative entropy based ensemble subnetwork to integrate the semantic and structural similarities. This subnetwork can adaptive learn the weights of the semantic and structural similarities at the training stage. To further enhance the discriminative capacity of the tracker, we propose a large-scale TIR video sequence dataset for training the proposed model. To the best of our knowledge, this is the first and the largest TIR object tracking training dataset to date. The proposed TIR dataset not only benefits the training for TIR object tracking but also can be applied to numerous TIR visual tasks. Extensive experimental results on three benchmarks demonstrate that the proposed algorithm performs favorably against the state-of-the-art methods.

DNA-binding proteins play a pivotal role in various intra- and extra-cellular activities ranging from DNA replication to gene expression control. Identification of DNA-binding proteins is one of the major challenges in the field of genome annotation. There have been several computational methods proposed in the literature to deal with the DNA-binding protein identification. However, most of them can't provide an invaluable knowledge base for our understanding of DNA-protein interactions.

Protein secondary structure is the three dimensional form of local segments of proteins and its prediction is an important problem in protein tertiary structure prediction. Developing computational approaches for protein secondary structure prediction is becoming increasingly urgent. We present a novel deep learning based model, referred to as CNNH_PSS, by using multi-scale CNN with highway. In CNNH_PSS, any two neighbor convolutional layers have a highway to deliver information from current layer to the output of the next one to keep local contexts. As lower layers extract local context while higher layers extract long-range interdependencies, the highways between neighbor layers allow CNNH_PSS to have ability to extract both local contexts and long-range interdependencies. We evaluate CNNH_PSS on two commonly used datasets: CB6133 and CB513. CNNH_PSS outperforms the multi-scale CNN without highway by at least 0.010 Q8 accuracy and also performs better than CNF, DeepCNF and SSpro8, which cannot extract long-range interdependencies, by at least 0.020 Q8 accuracy, demonstrating that both local contexts and long-range interdependencies are indeed useful for prediction. Furthermore, CNNH_PSS also performs better than GSM and DCRNN which need extra complex model to extract long-range interdependencies. It demonstrates that CNNH_PSS not only cost less computer resource, but also achieves better predicting performance. CNNH_PSS have ability to extracts both local contexts and long-range interdependencies by combing multi-scale CNN and highway network. The evaluations on common datasets and comparisons with state-of-the-art methods indicate that CNNH_PSS is an useful and efficient tool for protein secondary structure prediction.

Temporal and spatial contexts, characterizing target appearance variations and target-background differences, respectively, are crucial for improving the online adaptive ability and instance-level discriminative ability of object tracking. However, most existing trackers focus on either the temporal context or the spatial context during tracking and have not exploited these contexts simultaneously and effectively. In this paper, we propose a Spatial-TEmporal Memory (STEM) network to exploit these contexts jointly for object tracking. Specifically, we develop a key-value structured memory model equipped with a key-value index-based memory reading mechanism to model the spatial and temporal contexts simultaneously. To update the memory with new target states and ensure the diversity of the memory, we introduce a similarity-aware memory update scheme. In addition, we construct an entropy-guided ensemble strategy to fuse the prediction models based on these two contexts, such that these two contexts can be exploited to estimate the target state jointly. Extensive experimental results on eight challenging datasets, including OTB2015, TC128, UAV123, VOT2018, LaSOT, TrackingNet, GOT-10k, and OxUvA, demonstrate that the proposed method performs favorably against state-of-the-art trackers.

Prediction of DNA-binding residue is important for understanding the protein-DNA recognition mechanism. Many computational methods have been proposed for the prediction, but most of them do not consider the relationships of evolutionary information between residues. In this paper, we first propose a novel residue encoding method, referred to as the Position Specific Score Matrix (PSSM) Relation Transformation (PSSM-RT), to encode residues by utilizing the relationships of evolutionary information between residues. PDNA-62 and PDNA-224 are used to evaluate PSSM-RT and two existing PSSM encoding methods by five-fold cross-validation. Performance evaluations indicate that PSSM-RT is more effective than previous methods. This validates the point that the relationship of evolutionary information between residues is indeed useful in DNA-binding residue prediction. An ensemble learning classifier (EL_PSSM-RT) is also proposed by combining ensemble learning model and PSSM-RT to better handle the imbalance between binding and non-binding residues in datasets. EL_PSSM-RT is evaluated by five-fold cross-validation using PDNA-62 and PDNA-224 as well as two independent datasets TS-72 and TS-61. Performance comparisons with existing predictors on the four datasets demonstrate that EL_PSSM-RT is the best-performing method among all the predicting methods with improvement between 0.02–0.07 for MCC, 4.18–21.47% for ST and 0.013–0.131 for AUC. Furthermore, we analyze the importance of the pair-relationships extracted by PSSM-RT and the results validates the usefulness of PSSM-RT for encoding DNA-binding residues. We propose a novel prediction method for the prediction of DNA-binding residue with the inclusion of relationship of evolutionary information and ensemble learning. Performance evaluation shows that the relationship of evolutionary information between residues is indeed useful in DNA-binding residue prediction and ensemble learning can be used to address the data imbalance issue between binding and non-binding residues. A web service of EL_PSSM-RT ( http://hlt.hitsz.edu.cn:8080/PSSM-RT_SVM/ ) is provided for free access to the biological research community.