Dengue-suppressing Wolbachia strains are promising tools for arbovirus control, particularly as they have the potential to self-spread following local introductions. To test this, we followed the frequency of the transinfected Wolbachia strain wMel through Ae. aegypti in Cairns, Australia, following releases at 3 nonisolated locations within the city in early 2013. Spatial spread was analysed graphically using interpolation and by fitting a statistical model describing the position and width of the wave. For the larger 2 of the 3 releases (covering 0.97 km² and 0.52 km²), we observed slow but steady spatial spread, at about 100–200 m per year, roughly consistent with theoretical predictions. In contrast, the smallest release (0.11 km²) produced erratic temporal and spatial dynamics, with little evidence of spread after 2 years. This is consistent with the prediction concerning fitness-decreasing Wolbachia transinfections that a minimum release area is needed to achieve stable local establishment and spread in continuous habitats. Our graphical and likelihood analyses produced broadly consistent estimates of wave speed and wave width. Spread at all sites was spatially heterogeneous, suggesting that environmental heterogeneity will affect large-scale Wolbachia transformations of urban mosquito populations. The persistence and spread of Wolbachia in release areas meeting minimum area requirements indicates the promise of successful large-scale population transformation. Wolbachia are bacteria that live inside insect cells. In insects that act as viral vectors, Wolbachia can suppress virus transmission to new hosts. Wolbachia have been experimentally introduced into Aedes aegypti mosquito populations to reduce the transmission of dengue, Zika, and other arboviruses that cause human disease. Wolbachia invade populations by causing cytoplasmic incompatibility, a phenomenon whereby embryos from crosses between infected males and uninfected females fail to hatch. While Wolbachia have been shown to successfully invade and remain established in isolated Ae. aegypti populations, outward spread from urban release zones has not been previously documented. This is an important step in demonstrating that Wolbachia can be used to combat mosquito-borne infectious disease in cities. Here we describe Wolbachia spread from 2 introduction areas within Cairns in northeastern Australia at a rate of about 100–200 meters per year. Spread occurs only when introduction areas are sufficiently large. The slow rates of observed spread are broadly consistent with mathematical predictions based on estimated Ae. aegypti dispersal distances, Wolbachia dynamics, and effects seen in isolated populations. Spread is uneven and likely depends on local characteristics (e.g., barriers) that affect mosquito density and dispersal. Our data indicate that Wolbachia can be introduced locally in large cities, remain established where released, and slowly spread from release areas. These dynamics indicate that high Wolbachia infection frequencies can be established gradually across large urban areas through local releases.

Wolbachiaare endosymbionts found in many insects with the potential to suppress vectorborne diseases, particularly through interfering with pathogen transmission.Wolbachiastrains are highly variable in their effects on hosts, raising the issue of which attributes should be selected to ensure that the best strains are developed for disease control. This depends on their ability to suppress viral transmission, invade host populations, persist without loss of viral suppression and not interfere with other control strategies. The potential to achieve these objectives is likely to involve evolutionary constraints; viral suppression may be limited by the ability of infections to spread due to deleterious host fitness effects. However, there are exceptions to these patterns in both natural infections and in novel associations generated following interspecific transfer, suggesting that pathogen blockage, deleterious fitness effects and changes to reproductive biology might be at least partly decoupled to achieve ideal infection attributes. The stability of introducedWolbachiaand its effects on viral transmission remain unclear, but rapid evolutionary changes seem unlikely. Although deliberate transfers ofWolbachiaacross species remain particularly challenging, the availability of strains with desirable attributes should be expanded, taking advantage of the diversity available across thousands of strains in natural populations.

Genetic markers are widely used to understand the biology and population dynamics of disease vectors, but often markers are limited in the resolution they provide. In particular, the delineation of population structure, fine scale movement and patterns of relatedness are often obscured unless numerous markers are available. To address this issue in the major arbovirus vector, the yellow fever mosquito (Aedes aegypti), we used double digest Restriction-site Associated DNA (ddRAD) sequencing for the discovery of genome-wide single nucleotide polymorphisms (SNPs). We aimed to characterize the new SNP set and to test the resolution against previously described microsatellite markers in detecting broad and fine-scale genetic patterns in Ae. aegypti.

Significance: With over 40% of humans at risk from mosquito-borne diseases such as dengue, yellow fever, chikungunya, and Zika, the development of environmentally friendly mosquito-control tools is critical. The release of reproductively incompatible male mosquitoes carrying a Wolbachia bacterium can drive mating events that kill the eggs. Through replicated treatment and control experiments in northern Australia, regular releases of Aedes aegypti males infected with a Wolbachia from Aedes albopictus was shown to drive strong population suppression in mosaic populations of wild-type (no Wolbachia ) and w Mel- Wolbachia –carrying Ae. aegypti . In a demonstration of bidirectional incompatibility between different Wolbachia strains in the field, we also demonstrate that one season’s suppression experiment can also show an ongoing effect into the following season.

Domesticating Zika virus: Why hasn't Zika virus (ZIKV) disease caused as much devastation in Africa, its continent of origin, as it has in the Americas? Outside of Africa, this flavivirus is transmitted by a ubiquitous mosquito subspecies, Aedes aegypti aegypti , which emerged from the African forerunner subspecies A. aegypti formosus and acquired a preference for human blood and a peridomestic lifestyle. Now, this subspecies colonizes many intertropical cities, aided by climate change and human trash. Aubry et al. tested 14 laboratory mosquito colonies for their relative susceptibility to ZIKV. Quantitative trait locus mapping showed differences on chromosome 2 between mosquitoes from Gabon and Guadeloupe. Mouse infection experiments revealed that African mosquitoes transmitted a smaller virus inoculum than the South American insects. Increased susceptibility coupled with the ability of A. aegypti aegypti to breed in any discarded object containing water has amplified the problematic nature of this virus as it has circumnavigated the world. Science , this issue p. 991