Agricultural microbiology seeks to replace the use of agrochemicals with microbe-based products. Plant growth-promoting bacteria (PGPB) are often selected based on their functions in vitro , and then, their effect on plant development is tested. However, this approach neglects the study of their survival in soil, root-colonization, and the monitoring of beneficial functions in the rhizosphere. This could explain the recurrent lack of success in the transition from lab tests to field applications of natural isolates from novel habitats. In our recent studies, we found that native maize seeds from traditional agroecosystems carry endophytic bacterial communities that are dominated by members of the class Bacilli. As an approach to grasp their PGP potential, we developed protocols to test maize root-colonization of these natural isolates in 1) a short-term hydroponics assay in vitro and 2) a long-term assay in non-sterile soil pots. Our results show that in vitro biofilm formation was only partially associated to short-term colonization in vitro ; furthermore, long-term root-colonization in soil pots was not correlated to the in vitro assays. This work highlights the necessity to incorporate root-colonization assays as part of the research strategies in the search for PGPB natural isolates from unexplored habitats, towards the generation of inoculants with increased success in the field.

Floating wetlands have emerged as a sustainable alternative for improving water quality, and although some studies have investigated their performance, there is still much to be understood regarding their integration with energy-generating technologies. This study evaluated a combined system of floating wetlands and microbial fuel cells (MFCs) for treating real wastewater and generating bioelectricity. Experiments were conducted in batch mode to simulate application in natural water bodies, using real wastewater collected on different dates. As a result of the natural variability of the influent, initial chemical oxygen demand (COD) concentrations of 405 and 289 mg/L were observed. Performance was assessed in terms of organic matter and nitrogen removal, as well as voltage generation. COD removal efficiencies reached 50% and 69% for the higher and lower organic loads, respectively, indicating improved treatment at reduced concentrations. Maximum removals of 56% for ammoniacal nitrogen (NH₃-N) and 40% for total nitrogen (TN) were achieved, reflecting moderate nutrient removal capacity. Voltage generation was sustained for approximately 21 days, confirming stable bioelectrochemical activity, and power output was found to depend on the organic load serving as substrate for electrogenic microorganisms. Overall, the system represents a viable approach for wastewater treatment with the added benefit of energy recovery, although its performance is influenced by influent characteristics and operation conditions.

Performing a risk analysis by activity allows you to identify and control the risks associated with tasks in the work environment, reduce workplace hazards associated with the risks, as well as prevent or reduce injuries and illnesses in the worker. This article presents a standardized tool to perform a risk analysis by activity, each of the proposed stages is detailed, such as the description of the activity, identification of the risks present, description of the risks, identification of damage or potential effect, risk control hierarchy, identification of current controls, risk assessment and implementation of actions. Each of the stages takes you by the hand to systematically carry out a risk analysis by activity. Finally, the effectiveness of the tool implemented in carrying out a risk analysis is shown.