Background: Infectious pancreatic necrosis virus (IPNV) is a birnavirus that causes a lethal disease in both hatchery-reared juvenile salmonids and other non-salmonid fishes. IPNV has been classified into seven genogroups based on the analysis of the VP2/NS junction region of the viral A RNA segment. Methods: Ten organisms from two trout-rearing farms were used for viral isolation in RTG-2 cells. Cells were inoculated with samples from spleen, kidneys and pyloric caeca. The viral isolate was initially identified by electron microscopy, and confirmed by a commercial ELISA, RT-PCR and sequencing. A phylogenetic tree was constructed based on the deduced amino acids sequence of VP2. Results: An IPNV genogroup 1, labeled as EdoMex07, was isolated from a pool of renal tissues of five asymptomatic trout. The amino acid sequence analysis of VP2 showed that this IPNV presented the putative VP2 residue (221) already described in asymptomatic trout carriers. Conclusions: The EdoMex07 IPNV isolate belongs to genogroup 1, and has a VP2 phenotype which has been suggested to be involved in the establishment of the carrier state. This EdoMex07 IPNV is currently used as the standard positive control for detection of IPNV in rainbow trout farms in Mexico.

Botryosphaeria dieback, caused by various pathogens, including Lasiodiplodia spp., is a significant threat to grapevines. Lasiodiplodia brasiliensis has been frequently isolated from grapevines in Baja California and Sonora, Mexico, mainly from plants showing perennial cankers. While chemical and biological control strategies have been explored, alternatives such as organic amendments (OAs) have yet to be studied. This research aimed to evaluate the effect of OAs on the co‐occurrence of latent L . brasiliensis infection and the grapevine endophytic community using qPCR and metabarcoding analysis. Two OAs, compost cow manure and grapevine wood (CMW) and sludge kitchen waste and grapevine wood (KWW), were tested. Extracts were prepared by mixing 140 g of each OA with 210 mL of autoclaved water, shaking for 2 h, and filtering. Grapevine cuttings were soaked in the extracts for 10 days, then inoculated with a L . brasiliensis MXBCL28 conidiospore suspension (1 × 10 ⁵ spores mL ⁻¹ ) and incubated for 30 days. Among treatments, cuttings treated with 10% KWW extract exhibited the highest plant growth parameters, including the number and length of lateral shoots, inflorescences, and roots, with a 70% suppressive effect on L . brasiliensis . The KWW treatment also enhanced the abundance of beneficial endophytic genera, including Acinetobacter , Hymenobacter , Pseudomonas , and Flavobacterium , which were significantly associated with antagonistic activity against the pathogen. Meanwhile, genera such as Sphingomonas , Ochrobactrum , Azospirillum , and Rheinheimer appeared to be involved in plant‐induced systemic resistance. Furthermore, the dominant bacterial genus DMER64 and fungal genus Orbilia , both present in KWW, were recruited to colonize grapevine tissues, potentially promoting plant growth and physiological processes. Overall, the KWW sludge demonstrated multiple suppressive actions, such as the release of humic substances and biochemical compounds, which facilitate the recruitment of beneficial microorganisms in response to latent L . brasiliensis infection. These findings suggest that KWW is a promising alternative for managing grapevine pruning waste, effectively reducing GTD pathogen transmission while enhancing beneficial endophytic communities.

Organic amendments like compost are used as an alternative to diminish soil damages caused by chemical fertilization. Compost contains nutrients and microbial communities with some beneficial bacteria and fungi for the plant. A study was carried out in order to assess the effectiveness of compost on sorghum (Sorghum bicolor L. Moench). Compost and fermented organic amendments were produced for 60 days with domestic wastes and humus of plant debris from agriculture and gardening in polyethylene bioreactors of 200 L. A fertilization test was applied under randomized block design with one factor (fertilization treatment) to one-month-age sorghum plants in a land plot. Plant height and fresh weight variables were recorded and analyzed by ANOVA and Tukey test (p ≤ 0.05) at the end of three-months period. Results showed that plant height and fresh weight were higher in plants fertilized with compost than those fertilized with control (chemical fertilizer) and fermented organic amendments (p £ 0.05). Vegetable domestic wastes and humus are a good option as substrate to produce compost due to their high availability and low cost.