Background: Coxiella burnetii is a common zoonotic pathogen that can lead not only to acute or chronic Q fever but also to post-infectious syndromes, where autonomic nervous system (ANS) dysfunction has been suggested as a contributing mechanism. This study aimed to assess autonomic function in patients presenting with polymorphic symptoms, dysautonomia, or ME/CFS who had serological evidence of acute infection with Coxiella burnetii. Methods: A total of 156 participants were evaluated, including 100 seropositive patients and 56 matched controls. All subjects underwent standardized cardiovascular reflex tests (CART), beat-to-beat analysis of heart rate and blood pressure with baroreflex indices, 24 h Holter ECG with HRV assessment, and, in the Coxiella group, head-up tilt testing (HUTT). Results: A significantly higher prevalence of autonomic dysfunction was observed in the Coxiella group, predominantly affecting parasympathetic regulation, with abnormal CART scores, reduced LF power and baroreflex effectiveness, and a high rate of positive HUTT findings characterized by extreme blood pressure variability. Although long-term HRV measures did not differ significantly between groups, short-term indices consistently indicated ANS impairment. Conclusions: These findings suggest that Coxiella burnetii infection may trigger persistent autonomic dysfunction, potentially contributing to the development of ME/CFS and syncope in affected individuals. Further longitudinal studies are needed to clarify pathophysiological mechanisms and clinical implications.

Introduction/Objective. Bacterial vaginosis (BV) is the most common vaginal dysbiosis that enlarge a possibility of getting sexually transmitted infections (STI). The aims of this research is to examine association between BV and the nine most common causes of STIs (Chlamydia trachomatis, Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma spp, Trichomonas vaginalis, Neisseria gonorrhoeae, high-risk Human papilloma viruses and Herpes simplex virus type 1 and 2) and to determine if the presence of BV increase the probability of coinfection with any of the STIs microorganisms. Methods. This study involved 235 patients of reproductive age. One sample swab was collected each for vaginal and cervical testing. The vaginal swabs were used for detection of BV by RT-PCR test. The cervical swabs were used for the detection of the most common STIs which were tested by four different multiplex RT-PCR tests. Pearson’s χ2 test and Fisher’s probability test were used for statistical analysis of the results. Results. Comparison of the total number of STIs and the condition of the vaginal flora has shown that STIs are the most common in patients with BV 80 (89.9%). Women with BV have a higher frequency of infections with Ureaplasma spp. and M. Hominis, 71 (78.9%) and 50 (44.4%), respectively. The presence of detected STI pathogens and relation with the state of vaginal flora indicate that mono infections are present most often in patients with normal flora 51 (42.1%), while coinfections are mostly present in BV patients 50 (55.6%). Conclusion. This study has confirmed the association of M. hominis and Ureaplasma spp with BV as well as an association of coinfections with this dysbiosis. Better understanding of the association between various STIs and the status of vaginal flora is necessary to enable better diagnosis, prevention of diseases and women’s health protection.

Background: Bartonella henselae infection has been associated with a broad spectrum of neurological and autonomic manifestations, although its impact on autonomic nervous system function remains insufficiently characterized, and the aim of this study was to evaluate autonomic function in patients with polymorphic symptoms and Bartonella henselae IgM seroreactivity. Methods: In this cross-sectional study, 75 patients were compared with 75 age- and sex-matched healthy controls, and all participants underwent cardiovascular autonomic reflex testing, short-term (5 min) and long-term (24 h) heart rate variability analysis, and 24 h ambulatory blood pressure monitoring, while head-up tilt testing was performed in the Bartonella group. Results: Abnormal autonomic reflex tests were significantly more frequent in the Bartonella IgM-seroreactive group, particularly those reflecting parasympathetic function, while heart rate variability analysis demonstrated reduced high-frequency components and lower long-term variability indices, and head-up tilt testing revealed heterogeneous hemodynamic responses including orthostatic hypotension and pronounced blood pressure variability, with ambulatory monitoring additionally showing higher nighttime blood pressure values and reduced nocturnal dipping. Conclusions: These findings indicate a consistent pattern of autonomic imbalance characterized predominantly by parasympathetic impairment and altered blood pressure regulation in patients with Bartonella henselae IgM seroreactivity, although further studies are required to clarify underlying mechanisms and clinical implications.

Background/Aim. Bacterial vaginosis (BV) is one of the most common microbial dysbiosis, characterized by a decrease of Lactobacillus spp. with an increase of other anaerobic bacteria species (Gardnerella vaginalis, Atopobium vaginae, Prevotella spp, Mobiluncus spp etc.) causing serious gynecological and obstetric complications. Therefore, it is particularly important to have accurate and reliable diagnostic standards. The aim of this study is to compare the results of various diagnostic methods for detection of BV such as Amsel, Nugent, Ison and Hay criteria as well as multiplex quantitative RT-PCR. Methods. This study involved vaginal swabs from 235 patients of reproductive age. Nugent criteria were used as the ‘gold standard’ which was compared with Amsel and Ison/Hay criteria as well as multiplex quantitative RT-PCR based on the detection and quantification of Gardnerella vaginalis, Atopobium vaginae, Lactobacillus spp. and total concentration of bacterial DNA. Kappa coefficient was employed to measure agreement between tests. Results. Our analysis demonstrated excellent agreement between Ison/Hay criteria and Nugent scores (kappa = 0.95), good agreement between Amsel and Nugent criteria (kappa = 0.78), while between Nugent and RT-PCR criteria agreement was moderate (kappa = 0.59). Total determined agreements of Ison/Hay, Amsel and RT PCR against Nugent scores were 94.9%, 90.2% and 74%, respectively. Nugent methods classified the highest number of intermediate patients - 60 (25.2%). The largest number of BV patients was detected by the RT-PCR method, while the largest number of healthy patients was detected by Amsel criteria. Conclusion. We concluded that RT-PCR is the best choice for BV diagnosis because it is more efficient at differentiating patients with intermediate results. Compared to Amsel and Nugent methods that group patients in 2 or 3 categories, the RT-PCR method recognizes other conditions of vaginal flora that are important for correct diagnoses and application of better therapeutic approach, as well as preventing possible clinical consequences of this dysbiosis.