This study demonstrates that the generation of excess heat and isotopic water were replicated via a nuclear-like reaction or Low-Energy Nuclear Reactions (LENRs) in 11 reactors. Cross-verification using mass spectrometry (MS), Nuclear Magnetic Resonance (NMR), and cavity ring-down spectroscopy (CRDS) confirmed that the reacted water (byproduct of LENR) does indeed contain the ¹⁷O isotope. For mass production of ¹⁷O-enriched water, a batch-type internal circulation system integrating a storage tank and a circulation pump was developed. This setup allows continuous manufacturing of ¹⁷O-enriched water, with concentrations controlled via circulation time. Specifically, a 32 L system could yield an estimated concentration of 30 mol% ¹⁷O-enriched water within 290 h. To measure the high concentration of ¹⁷O-enriched water (H₂¹⁷O), we have developed a dilution method, which analyzes ¹⁷O NMR data of water produced by the reactor at different dilution ratios. Moreover, it was found that the LENR process produces stable, 200 nm nanobubbles (density 9.33 × 10⁶ particles/mL for the sample #185r12), which possibly consists of non-condensable gases (O₂ and CO₂) containing ¹⁷O. These persistent nanobubbles could enhance the long-term storage stability of isotopic water. A preliminary in vitro cell assay (MTT) using ¹⁷O-enriched water (0.35 mol%) revealed alterations in cancer cell metabolic activity, though further studies are required to evaluate potential biological mechanisms. Overall, this cost-effective production technology may provide ¹⁷O-enriched water for physical and biological research.

This study demonstrates the generation of excess energy and isotopes via Low-Energy Nuclear Reactions (LENR) of water in 11 reactors. Cross-verification using Mass Spectrometry (MS), Nuclear Magnetic Resonance (NMR), and Cavity Ring-Down Spectroscopy (CRDS) confirmed that 17O production concurrently occurs with excess energy release, establishing the reproduci-bility of the LENR process. For mass production of 17O-enriched water, a batch-type internal circulation system integrating a storage tank and a circulation pump was developed. This setup allows continuous manufacturing of 17O-enriched water, with concentrations controlled via cir-culation time. Specifically, a 32 L system successfully yielded 30 mol% 17O-enriched water within 290 h. For high-concentration measurement, a 17O NMR-verified dilution method was es-tablished. Moreover, the LENR process induces the formation of stable, 200 nm nanobubbles (density: 9.33 x 106 particles/mL for reactor #185r12), predominantly composed of 17O com-pounds of non-condensable gases (O2 and CO2). These persistent nanobubbles prevent gas loss, significantly enhancing the long-term storage stability and practical utility of the product. Uti-lizing this low-cost, mass-produced 17O-enriched water, a preliminary MTT assay revealed that 17O disrupts cancer cell metabolism, offering promising insights for future oncology research. Overall, this cost-effective mass-production technology facilitates the deployment of 17O in healthcare and biomedical sectors, promoting further academic and clinical exploration.

It has been observed in prior research that high thermal impact—resulting from a large temperature difference between hot water vapor and cold liquid water—can enhance the thermal performance of cavitation-induced low-energy nuclear reactions (LENRs) in water, with an estimated increase in the coefficient of performance (COP) of approximately 50% for every 100 °C temperature rise. The temperature of the hot water vapor is primarily determined by the boiler output, which typically represents the highest temperature source and plays a dominant role in reactor performance. In this study, a flow oscillator was designed as an thermal conditioning component for these potential LENR reactor systems using linear flow network analysis (LFNA) to generate flow resonance that elevates the hot vapor temperature, thereby increasing thermal impact and improving LENR performance. LFNA is based on the linearization of the fluid flow equations governing mass and momentum transport and utilizes a fluid-electric circuit analogy. For a fluid flow system, various components can be modeled using analogs of electrical resistance, capacitance, and inductance (R, C, and L), allowing the system behavior to be analyzed similarly to an RLC circuit. Through this analogy, flow resonance phenomena can be predicted, potentially enabling the generation of high-temperature and high-pressure responses that are beneficial to LENR processes. The analytical model was experimentally validated and subsequently applied in the LENR reactor design. The analytical result shows that an output temperature difference exceeding 350 °C can be achieved using a 0.5 m pulse tube at a 46 Hz triggering frequency with 20 kPa perturbation, which indicates a potential COP enhancement of 175% based on prior studies. The result provides a potential mechanism to significantly enhance the thermal impact conditions and promote LENR performance in water-based reactor systems.