Background/Objectives: Polysorbate 80 (PS80), a complex surfactant mixture, is widely recognized for its ability to enhance drug permeation across the blood–brain barrier (BBB). While this effect is generally attributed to the combined actions of its components, the specific contribution and potential selectivity of individual minor components remain poorly understood. This study therefore aimed to isolate and compare the primary minor components of PS80 to determine whether they uniformly enhance BBB permeation or exhibit drug-specific functions. Methods: In this research, four primary minor components of PS80—polyoxyethylene sorbitan monooleate (PSM), polyoxyethylene isosorbide monooleate (PIM), polyoxyethylene sorbitan dioleate (PSD), and a polyethylene glycol/polyoxyethylene sorbitan/polyoxyethylene isosorbide mixture (PEG/PS/PI mixture)—were isolated using preparative liquid-phase chromatography. Drug-loaded formulations were then prepared using the solvent evaporation method incorporating five model drugs: 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide (DiR, MW = 1013.39 Da), donepezil (MW = 379.49 Da), nimodipine (MW = 418.44 Da), chlorogenic acid (MW = 354.31 Da), and paclitaxel (MW = 853.92 Da). The permeability of these formulations across the BBB was evaluated in BALB/c mice after intravenous administration. Brain distribution of the lipophilic dye DiR was assessed using fluorescence imaging, whereas brain homogenate concentrations of therapeutic drugs were quantified by UPLC-MS/MS. Results: Results revealed that the enhancement of brain delivery was dependent on both the specific minor component and the drug. The PEG/PS/PI mixture specially enhanced the brain homogenate concentration of donepezil to 11.8 ± 1.2 ng/mL, representing a 6.9-fold enhancement, while PIM micelles increased the delivery of DiR, donepezil, and nimodipine. In contrast, PSM and PSD micelles improved transport of only DiR and donepezil. The broad performance of PIM suggests a more flexible formulation—a hypothesis that warrants further validation. Conversely, none of the different minor components enhanced the delivery of chlorogenic acid or paclitaxel, underscoring the critical role of specific drug–component interactions. Conclusions: This component-resolved insight challenges the conventional perception of PS80 and provides a rational framework for engineering precision brain-targeted delivery systems by selecting functional minor components.

Objective: To design and implement a proficiency testing (PT) scheme for evaluating the competence of inspection agencies in measuring temperature and relative humidity (RH) in clean environments. The study assesses laboratories’ mastery of testing standards and their operational proficiency with thermohygrometers. Methods: The PT combined a written examination (evaluating theoretical knowledge of standards) and a field assessment (assessing instrument operation and field testing accuracy). Homogeneity of temperature and RH samples was verified using relative standard deviation (RSD). Participant performance was scored using weighted criteria: the written examination (40 points) and field test (z-score evaluation, 60 points). Results: Sample homogeneity met PT requirements. All 54 participating laboratories achieved satisfactory results (100% satisfaction rate). Field test z-scores indicated high consistency: 98.1% of temperature results (|z|≤ 2) and 94.4% of RH results (|z|≤ 2) fell within satisfactory ranges. Conclusion: Laboratories demonstrated robust testing capabilities, though improvements are recommended in standard interpretation and instrument calibration practices. This PT framework establishes a novel quality assessment method for pharmaceutical clean environments and serves as a reference for similar evaluations.