Feline Panleukopenia (FPL) infection is caused by feline panleukopenia virus (FPV), and it is considered one of the most severe cat’s infectious diseases. Since there is no specific antiviral treatment for FPL, the therapeutic protocol usually is focused on fluid therapy and supportive care. However, filgrastim, a granulocyte colony-stimulating factor (G-CSF) used in human medicine to treat neutropenia and leukopenia, has been lately used in treating FPV disease, providing promising results. During January 2022 and September 2024, twenty-two cats diagnosed with feline panleukopenia virus were subjected to filgrastim (Zarzio^®, Sandoz, Kundl, Austria) administration at a dose of 6 µg/kg for 3 consecutive days. The 4th day was a break day, and in the 5th day, the complete blood count was repeated. White blood cells, lymphocytes, monocytes, neutrophils and eosinophils parameters improved after Zarzio^® administration, with a significant statistical difference (p < 0.01) when their values between day 1 (pre Zarzio^® administration) and day 5 (post Zarzio^® administration) were analysed. However, red blood cells, haemoglobin, haematocrit and platelets parameters registered a considerable reduction from day 1 to day 5 with a significant statistical difference (p < 0.01), considered as post-administration side effects. In our study, the survival rate following Zarzio^® administration was 100%, suggesting that the protocol involving three doses is effective in restoring the leukopenia and neutropenia.

Oral and cutaneous lesions are often diagnosed in cats and are associated with various pathogens. The objective of this study is the histological characterization of the cutaneous and oral lesions in cats. During 2023-2024, 11 cutaneous and oral lesions were identified, collected and analyzed at the Infectious Diseases and Pathological Anatomy Clinic of the Faculty of Veterinary Medicine of Iasi. The samples were collected from European breed cats, 6 female and 5 male cases. Lesions were more frequent in cats older than two years (n=7), followed by cats under 6 months of age (n=3) and cats aged between 6 months and 1,5 years (n= 1). At the same time, concurrent viral infections with Felin Parvovirus (n=5), Feline Calicivirus (n=3), Feline Herpesvirus (n=3),Feline Leukemia Virus (n=2) and one suspicion of Feline Perionitis Virus were associated prior the histopathological evaluation. Histopathologically, the inflammatory lesions were consisting in Gingivostomatits (necrotic and lymphoplasmacytic) (n=6) and in one case of cutaneous mastocytosis. Among neoplastic lesions, oral squamous cell carcinoma was the most common (n=2), followed by cutaneous cell carcinoma (n=1) and hemangiopericytoma (n=1). These results contribute to the understanding of feline cutaneous and oral pathology and show the importance of histopathological examination in their accurate diagnosis.

A five years’ Alpine goat breed was presented to the Faculty of Veterinary Medicine, Infectious Diseases Clinic with a history of recurrent mastitis, treated at home by the owner and a reduced appetite. During the physical examination, an acute inflammation of the mammary gland (right half) with oedema, congestion, sensitivity and pain at the palpation were noticed. The milk secretion was affected, with both halves presenting diminished secretion more pronounced on the right one, with serous appearance, separated in two layers: the upper part yellowish-green, while the inferior part with a jelly-like appearance and clots. The diagnosis was confirmed based on the clinical signs, culture and antibiogram assay. Additionally, a complete blood count was performed, revealing a mild lymphopenia. The treatment consisted in administration of bacteriophages through the papillary duct, accompanied by supportive care, represented by anti-inflammatory drug, vitamin therapy and fluid therapy. After 2 weeks of hospitalization, the goat totally recovered.

African swine fever (ASF) is a highly lethal viral disease affecting domestic pigs, while remaining asymptomatic in its natural reservoir hosts. The disease poses a substantial threat to the swine industry globally, with high mortality rates and significant economic implications, exacerbated by the lack of an effective vaccine. ASF is caused by the African swine fever virus (ASFV), a DNA virus with notable resistance to environmental factors, contributing to its persistence and complex transmission pathways. Transmission occurs both directly, through contact between infected and healthy animals, and indirectly, via contaminated carcasses, feed, or surfaces. This review highlights key aspects of ASF epidemiology, including the virus's environmental resilience and its ability to survive in feed and animal products under certain conditions. Research emphasizes the effectiveness of industrial processing methods such as pelletization, extrusion, and spray-drying in inactivating ASFV in feed materials. However, potential risks remain from direct contamination in areas inhabited by wild boars or due to improper waste management.

Mastitis, a significant concern in the dairy industry, poses severe economic and health challenges due to its impact on milk quality, yield, and herd productivity. This study investigated the microbiota of the mammary gland in cows diagnosed with clinical and subclinical mastitis, focusing on the identification and diversity of microbial pathogens. Milk samples (n=80) were collected from two dairy herds in Iasi County, Romania. Using advanced diagnostic techniques, including the California Mastitis Test (CMT), somatic cell count (SCC) analysis, and bacteriological isolation, a total of 49 microbial strains representing 19 genera were identified. The most frequently isolated pathogens included Streptococcus uberis (20.40%), Escherichia coli, Enterococcus faecium, Enterococcus cecorum (each 8.20%), and Staphylococcus spp., including S. aureus (2.04%), S. chromogenes, and S. epidermidis (each 4.08%). Future research will focus on resistance profiling and assessing the efficacy of novel antimicrobial agents to mitigate the impact of this multifaceted disease on dairy operations.

Alzheimer’s disease (AD) is recognized as a neuroglial disorder in which dysregulated glial responses contribute to cognitive decline. We investigated whether intrahippocampal transplantation of primary glial cells modifies cognitive trajectories in an amyloid-induced rat model of AD. Sprague–Dawley rats received bilateral hippocampal injections of pre-aggregated Aβ_1–42, followed by transplantation of primary astrocytes (AA) or microglia (AM). Memory was assessed using the Y-maze and novel object recognition (NOR) tests. Body weight and serum IL-6 and TNF-α were monitored for adverse effects, and GFAP, S100, Iba1 and TREM2 immunolabeling was quantified with QuPath. Mixed-effects models showed significant session and group x session effects on Y–maze spatial novelty recognition. Both treated groups improved compared with AD animals, with a stronger late effect in the AM group and a significant group × session × sex interaction. In the NOR test, both treated groups outperformed AD animals at the final session, without differences between them. GFAP (glial fibrillary acidic protein) immunoreactivity was reduced in the AM group but remained elevated in the AA group, while TREM2 increased after microglial and decreased after astrocyte transplantation. No adverse effects were detected in the monitored parameters. Intrahippocampal glial transplantation, particularly with microglia, may influence cognitive trajectories in established amyloid pathology.