Global agricultural production faces unprecedented challenges due to climate crisis, biodiversity loss, and increasing population pressure, while there is a growing demand for sustainable and eco-conscious food production systems. Traditional methods of crop protection like the use of synthetic chemical pesticides are becoming less effective due to the high resistance development in major insect pests. Moreover, their overuse has raised numerous environmental concerns. In this context, RNA interference (RNAi) has emerged as a promising and environmentally friendly alternative to traditional pesticides, with a more sustainable way of managing pests. This review systematically identifies promising RNAi target gene families for insect pest control, particularly key developmental genes. The selected genes were chosen based on demonstrated RNAi efficacy in at least three different insect species, emphasizing their broad applicability and potential impact. It also discusses the translation of RNAi technologies from laboratory research to field applications. It underscores the importance of moving beyond functional gene characterization to improving the efficiency and scalability of RNAi in real-world agricultural systems. This review systematically lists RNAi target genes and delivery methods in insect pests, identifies research gaps, and supports the development of RNAi-based biopesticides.

Food security faces significant challenges due to the growing threat of insect pests and global climate change, making it essential to adopt sustainable agricultural strategies. Plants possess intrinsic defense systems that are activated in response to herbivore attack, including the emission of specialized volatile organic compounds (VOC) known as herbivore-induced plant volatiles (HIPVs). These volatiles aid the foraging of natural enemies (predators and parasitoids), thereby contributing to biological control of pest populations. Harnessing these chemical defense mechanisms offers a promising avenue for pest management, although natural enemy attraction does not necessarily result in pest suppression or increased crop yield. This review synthesizes plant and insect-derived chemical signaling mechanisms with direct relevance to biological control in crop agroecosystems, including field crops, orchards, vegetable systems, and protected cultivation. We emphasize recent applied research while retaining selected foundational studies that established the principal concepts and mechanisms underlying chemically mediated pest management. Considering the importance of chemical signaling in sustainable agriculture, we propose four key research directions: (1) optimizing plant defense induction mechanisms; (2) integrating HIPVs with natural enemy pheromone-based attractants; (3) combining habitat management with the provision of plant-derived food resources; and (4) harnessing plant defenses through breeding and infochemical-based genetically modified crops. Additionally, we examine the potential impacts of environmental stressors on plant–herbivore chemical communication, underscoring their importance for resilient and environmentally sustainable agricultural systems.

The melon fly, Zeugodacus cucurbitae (Coquillett), is a destructive tephritid pest of cucurbit crops, and neonicotinoids remain important tools for its management. Although neonicotinoids primarily target nicotinic acetylcholine receptors (nAChRs), their possible effects on acetylcholinesterase (AChE)-mediated cholinergic homeostasis in Z. cucurbitae remain poorly understood. In this study, we molecularly characterized the Z. cucurbitae Ache gene and evaluated its expression and functional contribution under dinotefuran, acetamiprid, and thiamethoxam stress. Bioinformatics analyses confirmed conserved cholinesterase features, including the FGESAG catalytic motif, catalytic triad, choline-binding site, oxyanion hole, and conserved disulfide-bond residues. Temporal and tissue-specific expression profiling showed high Ache transcript abundance in eggs, third-instar larvae, and adult heads. Larval exposure to LC₅, LC₂₀, and LC₅₀ concentrations of the three neonicotinoids caused dose- and time-dependent mortality and significantly induced Ache expression, with dinotefuran producing the strongest transcriptional response. Parental exposure also impaired F₁ fecundity, hatchability, larval survival, and adult emergence, with acetamiprid and thiamethoxam causing stronger transgenerational effects than dinotefuran. Oral RNAi significantly increased larval susceptibility upon re-exposure to neonicotinoids after 72 h. These findings indicate that Ache contributes to cholinergic stress response and susceptibility modulation in Z. cucurbitae, supporting its possible potential as an RNAi target for integrated pest management (IPM). However, practical utility at the field level requires further evaluation.

The Cap ‘n’ collar isoform C (CncC) transcription factor, the insect ortholog of mammalian nuclear factor erythroid 2-related factor 2 (Nrf2), serves as a central regulator of redox homeostasis and xenobiotic detoxification. Increasing evidence demonstrates that the functions of CncC extend beyond stress responses to include metamorphosis, immunity, reproduction, aging, and the development of metabolic insecticide resistance. In this review, we synthesize recent advances in insect CncC biology, with a focus on the conserved CncC/Keap1-ARE signaling pathway and its role in the detoxification of phytochemicals and insecticides. We further discuss insect-specific regulatory interactions between CncC and ecdysone signaling, as well as the gut microbiota, and compare insect CncC with mammalian Nrf2. From an applied perspective, we discuss CncC as a target for resistance management, including strategies involving RNAi and small-molecule inhibitors, its potential application in inducible bioreactors, and its value as a biomarker in environmental risk assessment. We conclude by identifying key open questions, including tissue-specific regulation, fitness costs of constitutive activation, and field relevance of CncC-mediated resistance, and highlight how addressing these gaps can support the development of sustainable pest control strategies.